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Thermosensitive bFGF-Modified Hydrogel with Dental Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury

[Image: see text] Acute spinal cord injury (SCI) induces severe neuroinflammation, which increases intermediary filaments and neurodegeneration. Previous studies have shown that a basic fibroblast growth factor (bFGF) and dental pulp stem cells (DPSCs) contribute to a protective effect on injured ne...

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Autores principales: Albashari, Abdullkhaleg, He, Yan, Zhang, Yanni, Ali, Jihea, Lin, Feiou, Zheng, Zengming, Zhang, Keke, Cao, Yanfan, Xu, Chun, Luo, Lihua, Wang, Jianming, Ye, Qingsong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346236/
https://www.ncbi.nlm.nih.gov/pubmed/32656428
http://dx.doi.org/10.1021/acsomega.0c01379
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author Albashari, Abdullkhaleg
He, Yan
Zhang, Yanni
Ali, Jihea
Lin, Feiou
Zheng, Zengming
Zhang, Keke
Cao, Yanfan
Xu, Chun
Luo, Lihua
Wang, Jianming
Ye, Qingsong
author_facet Albashari, Abdullkhaleg
He, Yan
Zhang, Yanni
Ali, Jihea
Lin, Feiou
Zheng, Zengming
Zhang, Keke
Cao, Yanfan
Xu, Chun
Luo, Lihua
Wang, Jianming
Ye, Qingsong
author_sort Albashari, Abdullkhaleg
collection PubMed
description [Image: see text] Acute spinal cord injury (SCI) induces severe neuroinflammation, which increases intermediary filaments and neurodegeneration. Previous studies have shown that a basic fibroblast growth factor (bFGF) and dental pulp stem cells (DPSCs) contribute to a protective effect on injured neuronal cells, but the mechanism of SCI repair is still unclear. In this study, in situ heparin (HeP) hydrogel injection containing bFGF and DPSCs (HeP-bFGF-DPSCs), as well as in vitro studies of bFGF and DPSCs, proved an effective control over inflammation. The in vivo application of HeP-bFGF-DPSCs regulated inflammatory reactions and accelerated the nerve regeneration through microtubule stabilization and tissue vasculature. Our mechanistic investigation also showed that bFGF-DPSCs treatment inhibited microglia/macrophage proliferation and activation. Furthermore, HeP-bFGF-DPSCs prevented microglia/macrophage activation and reduced proinflammatory cytokine release. In this paper, we discovered that bFGF and DPSCs worked together to attenuate tissue inflammation of the injured spinal cord, resulting in a superior nerve repair. Our results indicated that a thermosensitive hydrogel delivering bFGF and DPSCs could serve as a promising treatment option for spinal cord injuries.
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spelling pubmed-73462362020-07-10 Thermosensitive bFGF-Modified Hydrogel with Dental Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury Albashari, Abdullkhaleg He, Yan Zhang, Yanni Ali, Jihea Lin, Feiou Zheng, Zengming Zhang, Keke Cao, Yanfan Xu, Chun Luo, Lihua Wang, Jianming Ye, Qingsong ACS Omega [Image: see text] Acute spinal cord injury (SCI) induces severe neuroinflammation, which increases intermediary filaments and neurodegeneration. Previous studies have shown that a basic fibroblast growth factor (bFGF) and dental pulp stem cells (DPSCs) contribute to a protective effect on injured neuronal cells, but the mechanism of SCI repair is still unclear. In this study, in situ heparin (HeP) hydrogel injection containing bFGF and DPSCs (HeP-bFGF-DPSCs), as well as in vitro studies of bFGF and DPSCs, proved an effective control over inflammation. The in vivo application of HeP-bFGF-DPSCs regulated inflammatory reactions and accelerated the nerve regeneration through microtubule stabilization and tissue vasculature. Our mechanistic investigation also showed that bFGF-DPSCs treatment inhibited microglia/macrophage proliferation and activation. Furthermore, HeP-bFGF-DPSCs prevented microglia/macrophage activation and reduced proinflammatory cytokine release. In this paper, we discovered that bFGF and DPSCs worked together to attenuate tissue inflammation of the injured spinal cord, resulting in a superior nerve repair. Our results indicated that a thermosensitive hydrogel delivering bFGF and DPSCs could serve as a promising treatment option for spinal cord injuries. American Chemical Society 2020-06-25 /pmc/articles/PMC7346236/ /pubmed/32656428 http://dx.doi.org/10.1021/acsomega.0c01379 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Albashari, Abdullkhaleg
He, Yan
Zhang, Yanni
Ali, Jihea
Lin, Feiou
Zheng, Zengming
Zhang, Keke
Cao, Yanfan
Xu, Chun
Luo, Lihua
Wang, Jianming
Ye, Qingsong
Thermosensitive bFGF-Modified Hydrogel with Dental Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury
title Thermosensitive bFGF-Modified Hydrogel with Dental Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury
title_full Thermosensitive bFGF-Modified Hydrogel with Dental Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury
title_fullStr Thermosensitive bFGF-Modified Hydrogel with Dental Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury
title_full_unstemmed Thermosensitive bFGF-Modified Hydrogel with Dental Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury
title_short Thermosensitive bFGF-Modified Hydrogel with Dental Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury
title_sort thermosensitive bfgf-modified hydrogel with dental pulp stem cells on neuroinflammation of spinal cord injury
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346236/
https://www.ncbi.nlm.nih.gov/pubmed/32656428
http://dx.doi.org/10.1021/acsomega.0c01379
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