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Thermosensitive bFGF-Modified Hydrogel with Dental Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury
[Image: see text] Acute spinal cord injury (SCI) induces severe neuroinflammation, which increases intermediary filaments and neurodegeneration. Previous studies have shown that a basic fibroblast growth factor (bFGF) and dental pulp stem cells (DPSCs) contribute to a protective effect on injured ne...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346236/ https://www.ncbi.nlm.nih.gov/pubmed/32656428 http://dx.doi.org/10.1021/acsomega.0c01379 |
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author | Albashari, Abdullkhaleg He, Yan Zhang, Yanni Ali, Jihea Lin, Feiou Zheng, Zengming Zhang, Keke Cao, Yanfan Xu, Chun Luo, Lihua Wang, Jianming Ye, Qingsong |
author_facet | Albashari, Abdullkhaleg He, Yan Zhang, Yanni Ali, Jihea Lin, Feiou Zheng, Zengming Zhang, Keke Cao, Yanfan Xu, Chun Luo, Lihua Wang, Jianming Ye, Qingsong |
author_sort | Albashari, Abdullkhaleg |
collection | PubMed |
description | [Image: see text] Acute spinal cord injury (SCI) induces severe neuroinflammation, which increases intermediary filaments and neurodegeneration. Previous studies have shown that a basic fibroblast growth factor (bFGF) and dental pulp stem cells (DPSCs) contribute to a protective effect on injured neuronal cells, but the mechanism of SCI repair is still unclear. In this study, in situ heparin (HeP) hydrogel injection containing bFGF and DPSCs (HeP-bFGF-DPSCs), as well as in vitro studies of bFGF and DPSCs, proved an effective control over inflammation. The in vivo application of HeP-bFGF-DPSCs regulated inflammatory reactions and accelerated the nerve regeneration through microtubule stabilization and tissue vasculature. Our mechanistic investigation also showed that bFGF-DPSCs treatment inhibited microglia/macrophage proliferation and activation. Furthermore, HeP-bFGF-DPSCs prevented microglia/macrophage activation and reduced proinflammatory cytokine release. In this paper, we discovered that bFGF and DPSCs worked together to attenuate tissue inflammation of the injured spinal cord, resulting in a superior nerve repair. Our results indicated that a thermosensitive hydrogel delivering bFGF and DPSCs could serve as a promising treatment option for spinal cord injuries. |
format | Online Article Text |
id | pubmed-7346236 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-73462362020-07-10 Thermosensitive bFGF-Modified Hydrogel with Dental Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury Albashari, Abdullkhaleg He, Yan Zhang, Yanni Ali, Jihea Lin, Feiou Zheng, Zengming Zhang, Keke Cao, Yanfan Xu, Chun Luo, Lihua Wang, Jianming Ye, Qingsong ACS Omega [Image: see text] Acute spinal cord injury (SCI) induces severe neuroinflammation, which increases intermediary filaments and neurodegeneration. Previous studies have shown that a basic fibroblast growth factor (bFGF) and dental pulp stem cells (DPSCs) contribute to a protective effect on injured neuronal cells, but the mechanism of SCI repair is still unclear. In this study, in situ heparin (HeP) hydrogel injection containing bFGF and DPSCs (HeP-bFGF-DPSCs), as well as in vitro studies of bFGF and DPSCs, proved an effective control over inflammation. The in vivo application of HeP-bFGF-DPSCs regulated inflammatory reactions and accelerated the nerve regeneration through microtubule stabilization and tissue vasculature. Our mechanistic investigation also showed that bFGF-DPSCs treatment inhibited microglia/macrophage proliferation and activation. Furthermore, HeP-bFGF-DPSCs prevented microglia/macrophage activation and reduced proinflammatory cytokine release. In this paper, we discovered that bFGF and DPSCs worked together to attenuate tissue inflammation of the injured spinal cord, resulting in a superior nerve repair. Our results indicated that a thermosensitive hydrogel delivering bFGF and DPSCs could serve as a promising treatment option for spinal cord injuries. American Chemical Society 2020-06-25 /pmc/articles/PMC7346236/ /pubmed/32656428 http://dx.doi.org/10.1021/acsomega.0c01379 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Albashari, Abdullkhaleg He, Yan Zhang, Yanni Ali, Jihea Lin, Feiou Zheng, Zengming Zhang, Keke Cao, Yanfan Xu, Chun Luo, Lihua Wang, Jianming Ye, Qingsong Thermosensitive bFGF-Modified Hydrogel with Dental Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury |
title | Thermosensitive bFGF-Modified Hydrogel with Dental
Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury |
title_full | Thermosensitive bFGF-Modified Hydrogel with Dental
Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury |
title_fullStr | Thermosensitive bFGF-Modified Hydrogel with Dental
Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury |
title_full_unstemmed | Thermosensitive bFGF-Modified Hydrogel with Dental
Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury |
title_short | Thermosensitive bFGF-Modified Hydrogel with Dental
Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury |
title_sort | thermosensitive bfgf-modified hydrogel with dental
pulp stem cells on neuroinflammation of spinal cord injury |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346236/ https://www.ncbi.nlm.nih.gov/pubmed/32656428 http://dx.doi.org/10.1021/acsomega.0c01379 |
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