Genome-wide identification of methylated CpG sites in nongenital cutaneous warts

BACKGROUND: Low-risk HPV infection has not been the subject of epigenetic investigation. The present study was carried out in order to investigate the methylation status of CpG sites in non-genital cutaneous warts. METHODS: Genomic DNA was extracted from 24 paired epidermal samples of warts and norm...

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Autores principales: AL-Eitan, Laith N., Alghamdi, Mansour A., Tarkhan, Amneh H., Al-Qarqaz, Firas A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346436/
https://www.ncbi.nlm.nih.gov/pubmed/32641122
http://dx.doi.org/10.1186/s12920-020-00745-6
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author AL-Eitan, Laith N.
Alghamdi, Mansour A.
Tarkhan, Amneh H.
Al-Qarqaz, Firas A.
author_facet AL-Eitan, Laith N.
Alghamdi, Mansour A.
Tarkhan, Amneh H.
Al-Qarqaz, Firas A.
author_sort AL-Eitan, Laith N.
collection PubMed
description BACKGROUND: Low-risk HPV infection has not been the subject of epigenetic investigation. The present study was carried out in order to investigate the methylation status of CpG sites in non-genital cutaneous warts. METHODS: Genomic DNA was extracted from 24 paired epidermal samples of warts and normal skin. DNA samples were bisulfite converted and underwent genome-wide methylation profiling using the Infinium MethylationEPIC BeadChip Kit. RESULTS: From a total of 844,234 CpG sites, 56,960 and 43,040 CpG sites were found to be hypo- and hypermethylated, respectively, in non-genital cutaneous warts. The most differentially methylated CpG sites in warts were located within the C10orf26, FAM83H-AS1, ZNF644, LINC00702, GSAP, STAT5A, HDAC4, NCALD, and EXOC4 genes. CONCLUSION: Non-genital cutaneous warts exhibit a unique CpG methylation signature.
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spelling pubmed-73464362020-07-14 Genome-wide identification of methylated CpG sites in nongenital cutaneous warts AL-Eitan, Laith N. Alghamdi, Mansour A. Tarkhan, Amneh H. Al-Qarqaz, Firas A. BMC Med Genomics Research Article BACKGROUND: Low-risk HPV infection has not been the subject of epigenetic investigation. The present study was carried out in order to investigate the methylation status of CpG sites in non-genital cutaneous warts. METHODS: Genomic DNA was extracted from 24 paired epidermal samples of warts and normal skin. DNA samples were bisulfite converted and underwent genome-wide methylation profiling using the Infinium MethylationEPIC BeadChip Kit. RESULTS: From a total of 844,234 CpG sites, 56,960 and 43,040 CpG sites were found to be hypo- and hypermethylated, respectively, in non-genital cutaneous warts. The most differentially methylated CpG sites in warts were located within the C10orf26, FAM83H-AS1, ZNF644, LINC00702, GSAP, STAT5A, HDAC4, NCALD, and EXOC4 genes. CONCLUSION: Non-genital cutaneous warts exhibit a unique CpG methylation signature. BioMed Central 2020-07-08 /pmc/articles/PMC7346436/ /pubmed/32641122 http://dx.doi.org/10.1186/s12920-020-00745-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
AL-Eitan, Laith N.
Alghamdi, Mansour A.
Tarkhan, Amneh H.
Al-Qarqaz, Firas A.
Genome-wide identification of methylated CpG sites in nongenital cutaneous warts
title Genome-wide identification of methylated CpG sites in nongenital cutaneous warts
title_full Genome-wide identification of methylated CpG sites in nongenital cutaneous warts
title_fullStr Genome-wide identification of methylated CpG sites in nongenital cutaneous warts
title_full_unstemmed Genome-wide identification of methylated CpG sites in nongenital cutaneous warts
title_short Genome-wide identification of methylated CpG sites in nongenital cutaneous warts
title_sort genome-wide identification of methylated cpg sites in nongenital cutaneous warts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346436/
https://www.ncbi.nlm.nih.gov/pubmed/32641122
http://dx.doi.org/10.1186/s12920-020-00745-6
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