Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936

Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed usi...

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Detalles Bibliográficos
Autores principales: Toombs, Jamie, Panther, Lindsay, Ornelas, Loren, Liu, Chunyan, Gomez, Emilda, Martín-Ibáñez, Raquel, Cox, Simon R., Ritchie, Stuart J., Harris, Sarah E., Taylor, Adele, Redmond, Paul, Russ, Tom C., Murphy, Lee, Cooper, James D., Burr, Karen, Selvaraj, Bhuvaneish T., Browne, Cathy, Svendsen, Clive N., Cowley, Sally A., Deary, Ian J., Chandran, Siddharthan, Spires-Jones, Tara L., Sareen, Dhruv
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347008/
https://www.ncbi.nlm.nih.gov/pubmed/32450543
http://dx.doi.org/10.1016/j.scr.2020.101851
Descripción
Sumario:Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating oriP/EBNA1 backbone plasmids expressing six iPSC reprogramming factors (OCT3/4 (POU5F1), SOX2, KLF4, L-Myc, shp53, Lin28, SV40LT). All lines demonstrated STR matched karyotype and pluripotency was validated by multiple methods. These iPSC lines are a valuable resource to study molecular mechanisms underlying individual differences in cognitive ageing and resilience to age-related neurodegenerative diseases.

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