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Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936
Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed usi...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347008/ https://www.ncbi.nlm.nih.gov/pubmed/32450543 http://dx.doi.org/10.1016/j.scr.2020.101851 |
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| author | Toombs, Jamie Panther, Lindsay Ornelas, Loren Liu, Chunyan Gomez, Emilda Martín-Ibáñez, Raquel Cox, Simon R. Ritchie, Stuart J. Harris, Sarah E. Taylor, Adele Redmond, Paul Russ, Tom C. Murphy, Lee Cooper, James D. Burr, Karen Selvaraj, Bhuvaneish T. Browne, Cathy Svendsen, Clive N. Cowley, Sally A. Deary, Ian J. Chandran, Siddharthan Spires-Jones, Tara L. Sareen, Dhruv |
| author_facet | Toombs, Jamie Panther, Lindsay Ornelas, Loren Liu, Chunyan Gomez, Emilda Martín-Ibáñez, Raquel Cox, Simon R. Ritchie, Stuart J. Harris, Sarah E. Taylor, Adele Redmond, Paul Russ, Tom C. Murphy, Lee Cooper, James D. Burr, Karen Selvaraj, Bhuvaneish T. Browne, Cathy Svendsen, Clive N. Cowley, Sally A. Deary, Ian J. Chandran, Siddharthan Spires-Jones, Tara L. Sareen, Dhruv |
| author_sort | Toombs, Jamie |
| collection | PubMed |
| description | Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating oriP/EBNA1 backbone plasmids expressing six iPSC reprogramming factors (OCT3/4 (POU5F1), SOX2, KLF4, L-Myc, shp53, Lin28, SV40LT). All lines demonstrated STR matched karyotype and pluripotency was validated by multiple methods. These iPSC lines are a valuable resource to study molecular mechanisms underlying individual differences in cognitive ageing and resilience to age-related neurodegenerative diseases. |
| format | Online Article Text |
| id | pubmed-7347008 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | Elsevier |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-73470082020-07-14 Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936 Toombs, Jamie Panther, Lindsay Ornelas, Loren Liu, Chunyan Gomez, Emilda Martín-Ibáñez, Raquel Cox, Simon R. Ritchie, Stuart J. Harris, Sarah E. Taylor, Adele Redmond, Paul Russ, Tom C. Murphy, Lee Cooper, James D. Burr, Karen Selvaraj, Bhuvaneish T. Browne, Cathy Svendsen, Clive N. Cowley, Sally A. Deary, Ian J. Chandran, Siddharthan Spires-Jones, Tara L. Sareen, Dhruv Stem Cell Res Article Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating oriP/EBNA1 backbone plasmids expressing six iPSC reprogramming factors (OCT3/4 (POU5F1), SOX2, KLF4, L-Myc, shp53, Lin28, SV40LT). All lines demonstrated STR matched karyotype and pluripotency was validated by multiple methods. These iPSC lines are a valuable resource to study molecular mechanisms underlying individual differences in cognitive ageing and resilience to age-related neurodegenerative diseases. Elsevier 2020-07 /pmc/articles/PMC7347008/ /pubmed/32450543 http://dx.doi.org/10.1016/j.scr.2020.101851 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| spellingShingle | Article Toombs, Jamie Panther, Lindsay Ornelas, Loren Liu, Chunyan Gomez, Emilda Martín-Ibáñez, Raquel Cox, Simon R. Ritchie, Stuart J. Harris, Sarah E. Taylor, Adele Redmond, Paul Russ, Tom C. Murphy, Lee Cooper, James D. Burr, Karen Selvaraj, Bhuvaneish T. Browne, Cathy Svendsen, Clive N. Cowley, Sally A. Deary, Ian J. Chandran, Siddharthan Spires-Jones, Tara L. Sareen, Dhruv Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936 |
| title | Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936 |
| title_full | Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936 |
| title_fullStr | Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936 |
| title_full_unstemmed | Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936 |
| title_short | Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936 |
| title_sort | generation of twenty four induced pluripotent stem cell lines from twenty four members of the lothian birth cohort 1936 |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347008/ https://www.ncbi.nlm.nih.gov/pubmed/32450543 http://dx.doi.org/10.1016/j.scr.2020.101851 |
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