Rates of homology directed repair of CRISPR-Cas9 induced double strand breaks are lower in naïve compared to primed human pluripotent stem cells

Gene editing in human pluripotent stem cells (hPSC) is a powerful tool for understanding biology, for drug discovery and gene therapy. Naïve hPSC have been suggested to be superior for gene editing compared to conventional ‘primed’ hPSC. Using droplet digital PCR, we uncover the kinetics of Cas9-ind...

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Detalles Bibliográficos
Autores principales: Dodsworth, Benjamin T., Hatje, Klas, Meyer, Claas Aiko, Flynn, Rowan, Cowley, Sally A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347009/
https://www.ncbi.nlm.nih.gov/pubmed/32521498
http://dx.doi.org/10.1016/j.scr.2020.101852

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