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Green fabricated zinc oxide nanoformulated media enhanced callus induction and regeneration dynamics of Panicum virgatum L.

The current study focuses on the usage of bio synthesized zinc oxide nanoparticles to increase the tissue culture efficiency of important forage grass Panicum virgatum. Zinc being a micronutrient enhanced the callogenesis and regeneration efficiency of Panicum virgatum at different concentrations. H...

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Autores principales: Shafique, Saima, Jabeen, Nyla, Ahmad, Khawaja Shafique, Irum, Samra, Anwaar, Sadaf, Ahmad, Naeem, Alam, Sadia, Ilyas, Muhammad, Khan, Talha Farooq, Hussain, Syed Zaheer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347099/
https://www.ncbi.nlm.nih.gov/pubmed/32645102
http://dx.doi.org/10.1371/journal.pone.0230464
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author Shafique, Saima
Jabeen, Nyla
Ahmad, Khawaja Shafique
Irum, Samra
Anwaar, Sadaf
Ahmad, Naeem
Alam, Sadia
Ilyas, Muhammad
Khan, Talha Farooq
Hussain, Syed Zaheer
author_facet Shafique, Saima
Jabeen, Nyla
Ahmad, Khawaja Shafique
Irum, Samra
Anwaar, Sadaf
Ahmad, Naeem
Alam, Sadia
Ilyas, Muhammad
Khan, Talha Farooq
Hussain, Syed Zaheer
author_sort Shafique, Saima
collection PubMed
description The current study focuses on the usage of bio synthesized zinc oxide nanoparticles to increase the tissue culture efficiency of important forage grass Panicum virgatum. Zinc being a micronutrient enhanced the callogenesis and regeneration efficiency of Panicum virgatum at different concentrations. Here, we synthesized zinc oxide nanoparticles through Cymbopogon citratus leaves extract to evaluate the effect of zinc oxide nanoparticles on plant regeneration ability in switchgrass. X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) validate phase purity of green synthesize Zinc oxide nanoparticles whereas, electron microscopy (SEM) has illustrated the average size of particle 50±4 nm with hexagonal rod like shape. Energy dispersive spectroscopy X-ray (EDS) depicted major peaks of Zn (92.68%) while minor peaks refer to Oxygen (7.32%). ZnO-NPs demonstrated the incredibly promising results against callogenesis. Biosynthesized ZnO-NPs at optimum concentration showed very promising effect on plant regeneration ability. Both the explants, seeds and nodes showed dose dependent response and upon high doses exceeding 40 mg/L the results were recorded negative, whereas at 30 mg/L both explants demonstrated 70% and 76% regeneration frequency. The results conclude that ZnO-NPs enhance the plant growth and development and tailored the nutritive properties at nano-scale. Furthermore, eco-friendly approach of ZnO-NPs synthesis is strongly believed to improve in vitro regeneration frequencies in several other monocot plants.
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spelling pubmed-73470992020-07-17 Green fabricated zinc oxide nanoformulated media enhanced callus induction and regeneration dynamics of Panicum virgatum L. Shafique, Saima Jabeen, Nyla Ahmad, Khawaja Shafique Irum, Samra Anwaar, Sadaf Ahmad, Naeem Alam, Sadia Ilyas, Muhammad Khan, Talha Farooq Hussain, Syed Zaheer PLoS One Research Article The current study focuses on the usage of bio synthesized zinc oxide nanoparticles to increase the tissue culture efficiency of important forage grass Panicum virgatum. Zinc being a micronutrient enhanced the callogenesis and regeneration efficiency of Panicum virgatum at different concentrations. Here, we synthesized zinc oxide nanoparticles through Cymbopogon citratus leaves extract to evaluate the effect of zinc oxide nanoparticles on plant regeneration ability in switchgrass. X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) validate phase purity of green synthesize Zinc oxide nanoparticles whereas, electron microscopy (SEM) has illustrated the average size of particle 50±4 nm with hexagonal rod like shape. Energy dispersive spectroscopy X-ray (EDS) depicted major peaks of Zn (92.68%) while minor peaks refer to Oxygen (7.32%). ZnO-NPs demonstrated the incredibly promising results against callogenesis. Biosynthesized ZnO-NPs at optimum concentration showed very promising effect on plant regeneration ability. Both the explants, seeds and nodes showed dose dependent response and upon high doses exceeding 40 mg/L the results were recorded negative, whereas at 30 mg/L both explants demonstrated 70% and 76% regeneration frequency. The results conclude that ZnO-NPs enhance the plant growth and development and tailored the nutritive properties at nano-scale. Furthermore, eco-friendly approach of ZnO-NPs synthesis is strongly believed to improve in vitro regeneration frequencies in several other monocot plants. Public Library of Science 2020-07-09 /pmc/articles/PMC7347099/ /pubmed/32645102 http://dx.doi.org/10.1371/journal.pone.0230464 Text en © 2020 Shafique et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Shafique, Saima
Jabeen, Nyla
Ahmad, Khawaja Shafique
Irum, Samra
Anwaar, Sadaf
Ahmad, Naeem
Alam, Sadia
Ilyas, Muhammad
Khan, Talha Farooq
Hussain, Syed Zaheer
Green fabricated zinc oxide nanoformulated media enhanced callus induction and regeneration dynamics of Panicum virgatum L.
title Green fabricated zinc oxide nanoformulated media enhanced callus induction and regeneration dynamics of Panicum virgatum L.
title_full Green fabricated zinc oxide nanoformulated media enhanced callus induction and regeneration dynamics of Panicum virgatum L.
title_fullStr Green fabricated zinc oxide nanoformulated media enhanced callus induction and regeneration dynamics of Panicum virgatum L.
title_full_unstemmed Green fabricated zinc oxide nanoformulated media enhanced callus induction and regeneration dynamics of Panicum virgatum L.
title_short Green fabricated zinc oxide nanoformulated media enhanced callus induction and regeneration dynamics of Panicum virgatum L.
title_sort green fabricated zinc oxide nanoformulated media enhanced callus induction and regeneration dynamics of panicum virgatum l.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347099/
https://www.ncbi.nlm.nih.gov/pubmed/32645102
http://dx.doi.org/10.1371/journal.pone.0230464
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