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Intra-host Ebola viral adaption during human infection
The onsite next generation sequencing (NGS) of Ebola virus (EBOV) genomes during the 2013–2016 Ebola epidemic in Western Africa provides an opportunity to trace the origin, transmission, and evolution of this virus. Herein, we have diagnosed a cohort of EBOV patients in Sierra Leone in 2015, during...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Chinese Medical Association Publishing House. Published by Elsevier B.V.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347341/ https://www.ncbi.nlm.nih.gov/pubmed/32835207 http://dx.doi.org/10.1016/j.bsheal.2019.02.001 |
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author | Liu, William J. Shi, Weifeng Zhu, Wuyang Jin, Cong Zou, Shumei Wang, Ji Ke, Yuehua Li, Xiaofeng Liu, Mi Hu, Tao Fan, Hang Tong, Yigang Zhao, Xiang Chen, Wenbin Zhao, Yuhui Liu, Di Wong, Gary Chen, Chengchao Geng, Chunyu Xie, Weiwei Jiang, Hui Kamara, Idrissa Laybor Kamara, Abdul Lebby, Matt Kargbo, Brima Qiu, Xiangguo Wang, Yu Liang, Xiaofeng Liang, Mifang Dong, Xiaoping Wu, Guizhen Gao, George F. Shu, Yuelong |
author_facet | Liu, William J. Shi, Weifeng Zhu, Wuyang Jin, Cong Zou, Shumei Wang, Ji Ke, Yuehua Li, Xiaofeng Liu, Mi Hu, Tao Fan, Hang Tong, Yigang Zhao, Xiang Chen, Wenbin Zhao, Yuhui Liu, Di Wong, Gary Chen, Chengchao Geng, Chunyu Xie, Weiwei Jiang, Hui Kamara, Idrissa Laybor Kamara, Abdul Lebby, Matt Kargbo, Brima Qiu, Xiangguo Wang, Yu Liang, Xiaofeng Liang, Mifang Dong, Xiaoping Wu, Guizhen Gao, George F. Shu, Yuelong |
author_sort | Liu, William J. |
collection | PubMed |
description | The onsite next generation sequencing (NGS) of Ebola virus (EBOV) genomes during the 2013–2016 Ebola epidemic in Western Africa provides an opportunity to trace the origin, transmission, and evolution of this virus. Herein, we have diagnosed a cohort of EBOV patients in Sierra Leone in 2015, during the late phase of the outbreak. The surviving EBOV patients had a recovery process characterized by decreasing viremia, fever, and biochemical parameters. EBOV genomes sequenced through the longitudinal blood samples of these patients showed dynamic intra-host substitutions of the virus during acute infection, including the previously described short stretches of 13 serial T>C mutations. Remarkably, within individual patients, samples collected during the early phase of infection possessed Ts at these nucleotide sites, whereas they were replaced by Cs in samples collected in the later phase, suggesting that these short stretches of T>C mutations could emerge independently. In addition, up to a total of 35 nucleotide sites spanning the EBOV genome were mutated coincidently. Our study showed the dynamic intra-host adaptation of EBOV during patient recovery and gave more insight into the complex EBOV-host interactions. |
format | Online Article Text |
id | pubmed-7347341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Chinese Medical Association Publishing House. Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73473412020-07-10 Intra-host Ebola viral adaption during human infection Liu, William J. Shi, Weifeng Zhu, Wuyang Jin, Cong Zou, Shumei Wang, Ji Ke, Yuehua Li, Xiaofeng Liu, Mi Hu, Tao Fan, Hang Tong, Yigang Zhao, Xiang Chen, Wenbin Zhao, Yuhui Liu, Di Wong, Gary Chen, Chengchao Geng, Chunyu Xie, Weiwei Jiang, Hui Kamara, Idrissa Laybor Kamara, Abdul Lebby, Matt Kargbo, Brima Qiu, Xiangguo Wang, Yu Liang, Xiaofeng Liang, Mifang Dong, Xiaoping Wu, Guizhen Gao, George F. Shu, Yuelong Biosaf Health Article The onsite next generation sequencing (NGS) of Ebola virus (EBOV) genomes during the 2013–2016 Ebola epidemic in Western Africa provides an opportunity to trace the origin, transmission, and evolution of this virus. Herein, we have diagnosed a cohort of EBOV patients in Sierra Leone in 2015, during the late phase of the outbreak. The surviving EBOV patients had a recovery process characterized by decreasing viremia, fever, and biochemical parameters. EBOV genomes sequenced through the longitudinal blood samples of these patients showed dynamic intra-host substitutions of the virus during acute infection, including the previously described short stretches of 13 serial T>C mutations. Remarkably, within individual patients, samples collected during the early phase of infection possessed Ts at these nucleotide sites, whereas they were replaced by Cs in samples collected in the later phase, suggesting that these short stretches of T>C mutations could emerge independently. In addition, up to a total of 35 nucleotide sites spanning the EBOV genome were mutated coincidently. Our study showed the dynamic intra-host adaptation of EBOV during patient recovery and gave more insight into the complex EBOV-host interactions. Chinese Medical Association Publishing House. Published by Elsevier B.V. 2019-06 2019-02-20 /pmc/articles/PMC7347341/ /pubmed/32835207 http://dx.doi.org/10.1016/j.bsheal.2019.02.001 Text en © 2019 Chinese Medical Association Publishing House. Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Liu, William J. Shi, Weifeng Zhu, Wuyang Jin, Cong Zou, Shumei Wang, Ji Ke, Yuehua Li, Xiaofeng Liu, Mi Hu, Tao Fan, Hang Tong, Yigang Zhao, Xiang Chen, Wenbin Zhao, Yuhui Liu, Di Wong, Gary Chen, Chengchao Geng, Chunyu Xie, Weiwei Jiang, Hui Kamara, Idrissa Laybor Kamara, Abdul Lebby, Matt Kargbo, Brima Qiu, Xiangguo Wang, Yu Liang, Xiaofeng Liang, Mifang Dong, Xiaoping Wu, Guizhen Gao, George F. Shu, Yuelong Intra-host Ebola viral adaption during human infection |
title | Intra-host Ebola viral adaption during human infection |
title_full | Intra-host Ebola viral adaption during human infection |
title_fullStr | Intra-host Ebola viral adaption during human infection |
title_full_unstemmed | Intra-host Ebola viral adaption during human infection |
title_short | Intra-host Ebola viral adaption during human infection |
title_sort | intra-host ebola viral adaption during human infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347341/ https://www.ncbi.nlm.nih.gov/pubmed/32835207 http://dx.doi.org/10.1016/j.bsheal.2019.02.001 |
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