Deletion of TRPV4 enhances in vitro wound healing of murine esophageal keratinocytes

Transient receptor potential vanilloid 4 (TRPV4) is a non-selective cation channel that is widely expressed in different body tissues and plays several physiological roles. This channel is highly expressed in esophageal keratinocytes where its activation mediates ATP release. However, whether TRPV4...

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Autores principales: Boudaka, Ammar, Saito, Claire T., Tominaga, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347589/
https://www.ncbi.nlm.nih.gov/pubmed/32647282
http://dx.doi.org/10.1038/s41598-020-68269-8
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author Boudaka, Ammar
Saito, Claire T.
Tominaga, Makoto
author_facet Boudaka, Ammar
Saito, Claire T.
Tominaga, Makoto
author_sort Boudaka, Ammar
collection PubMed
description Transient receptor potential vanilloid 4 (TRPV4) is a non-selective cation channel that is widely expressed in different body tissues and plays several physiological roles. This channel is highly expressed in esophageal keratinocytes where its activation mediates ATP release. However, whether TRPV4 has a role in wound healing of esophageal keratinocytes is unclear. In this study, we demonstrated that both cell migration and proliferation were slower in wild-type esophageal keratinocytes compared to cells having TRPV4 knockout. Our results suggest that TRPV4-mediated release of ATP from esophageal keratinocytes contributes to a decrease in the rate of in vitro wound healing via the ATP degradation product adenosine, which acts on A(2B) adenosine receptors.
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spelling pubmed-73475892020-07-10 Deletion of TRPV4 enhances in vitro wound healing of murine esophageal keratinocytes Boudaka, Ammar Saito, Claire T. Tominaga, Makoto Sci Rep Article Transient receptor potential vanilloid 4 (TRPV4) is a non-selective cation channel that is widely expressed in different body tissues and plays several physiological roles. This channel is highly expressed in esophageal keratinocytes where its activation mediates ATP release. However, whether TRPV4 has a role in wound healing of esophageal keratinocytes is unclear. In this study, we demonstrated that both cell migration and proliferation were slower in wild-type esophageal keratinocytes compared to cells having TRPV4 knockout. Our results suggest that TRPV4-mediated release of ATP from esophageal keratinocytes contributes to a decrease in the rate of in vitro wound healing via the ATP degradation product adenosine, which acts on A(2B) adenosine receptors. Nature Publishing Group UK 2020-07-09 /pmc/articles/PMC7347589/ /pubmed/32647282 http://dx.doi.org/10.1038/s41598-020-68269-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Boudaka, Ammar
Saito, Claire T.
Tominaga, Makoto
Deletion of TRPV4 enhances in vitro wound healing of murine esophageal keratinocytes
title Deletion of TRPV4 enhances in vitro wound healing of murine esophageal keratinocytes
title_full Deletion of TRPV4 enhances in vitro wound healing of murine esophageal keratinocytes
title_fullStr Deletion of TRPV4 enhances in vitro wound healing of murine esophageal keratinocytes
title_full_unstemmed Deletion of TRPV4 enhances in vitro wound healing of murine esophageal keratinocytes
title_short Deletion of TRPV4 enhances in vitro wound healing of murine esophageal keratinocytes
title_sort deletion of trpv4 enhances in vitro wound healing of murine esophageal keratinocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347589/
https://www.ncbi.nlm.nih.gov/pubmed/32647282
http://dx.doi.org/10.1038/s41598-020-68269-8
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