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Nanosensors based on LSPR are able to serologically differentiate dengue from Zika infections
The Flaviviridae virus family was named after the Yellow-fever virus, and the latin term flavi means “of golden color”. Dengue, caused by Dengue virus (DENV), is one of the most important infectious diseases worldwide. A sensitive and differential diagnosis is crucial for patient management, especia...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347616/ https://www.ncbi.nlm.nih.gov/pubmed/32647259 http://dx.doi.org/10.1038/s41598-020-68357-9 |
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author | Versiani, Alice F. Martins, Estefânia M. N. Andrade, Lidia M. Cox, Laura Pereira, Glauco C. Barbosa-Stancioli, Edel F. Nogueira, Mauricio L. Ladeira, Luiz O. da Fonseca, Flávio G. |
author_facet | Versiani, Alice F. Martins, Estefânia M. N. Andrade, Lidia M. Cox, Laura Pereira, Glauco C. Barbosa-Stancioli, Edel F. Nogueira, Mauricio L. Ladeira, Luiz O. da Fonseca, Flávio G. |
author_sort | Versiani, Alice F. |
collection | PubMed |
description | The Flaviviridae virus family was named after the Yellow-fever virus, and the latin term flavi means “of golden color”. Dengue, caused by Dengue virus (DENV), is one of the most important infectious diseases worldwide. A sensitive and differential diagnosis is crucial for patient management, especially due to the occurrence of serological cross-reactivity to other co-circulating flaviviruses. This became particularly important with the emergence of Zika virus (ZIKV) in areas were DENV seroprevalence was already high. We developed a sensitive and specific diagnostic test based on gold nanorods (GNR) functionalized with DENV proteins as nanosensors. These were able to detect as little as one picogram of anti-DENV monoclonal antibodies and highly diluted DENV-positive human sera. The nanosensors could differentiate DENV-positive sera from other flavivirus-infected patients, including ZIKV, and were even able to distinguish which DENV serotype infected individual patients. Readouts are obtained in ELISA-plate spectrophotometers without the need of specific devices. |
format | Online Article Text |
id | pubmed-7347616 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-73476162020-07-10 Nanosensors based on LSPR are able to serologically differentiate dengue from Zika infections Versiani, Alice F. Martins, Estefânia M. N. Andrade, Lidia M. Cox, Laura Pereira, Glauco C. Barbosa-Stancioli, Edel F. Nogueira, Mauricio L. Ladeira, Luiz O. da Fonseca, Flávio G. Sci Rep Article The Flaviviridae virus family was named after the Yellow-fever virus, and the latin term flavi means “of golden color”. Dengue, caused by Dengue virus (DENV), is one of the most important infectious diseases worldwide. A sensitive and differential diagnosis is crucial for patient management, especially due to the occurrence of serological cross-reactivity to other co-circulating flaviviruses. This became particularly important with the emergence of Zika virus (ZIKV) in areas were DENV seroprevalence was already high. We developed a sensitive and specific diagnostic test based on gold nanorods (GNR) functionalized with DENV proteins as nanosensors. These were able to detect as little as one picogram of anti-DENV monoclonal antibodies and highly diluted DENV-positive human sera. The nanosensors could differentiate DENV-positive sera from other flavivirus-infected patients, including ZIKV, and were even able to distinguish which DENV serotype infected individual patients. Readouts are obtained in ELISA-plate spectrophotometers without the need of specific devices. Nature Publishing Group UK 2020-07-09 /pmc/articles/PMC7347616/ /pubmed/32647259 http://dx.doi.org/10.1038/s41598-020-68357-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Versiani, Alice F. Martins, Estefânia M. N. Andrade, Lidia M. Cox, Laura Pereira, Glauco C. Barbosa-Stancioli, Edel F. Nogueira, Mauricio L. Ladeira, Luiz O. da Fonseca, Flávio G. Nanosensors based on LSPR are able to serologically differentiate dengue from Zika infections |
title | Nanosensors based on LSPR are able to serologically differentiate dengue from Zika infections |
title_full | Nanosensors based on LSPR are able to serologically differentiate dengue from Zika infections |
title_fullStr | Nanosensors based on LSPR are able to serologically differentiate dengue from Zika infections |
title_full_unstemmed | Nanosensors based on LSPR are able to serologically differentiate dengue from Zika infections |
title_short | Nanosensors based on LSPR are able to serologically differentiate dengue from Zika infections |
title_sort | nanosensors based on lspr are able to serologically differentiate dengue from zika infections |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347616/ https://www.ncbi.nlm.nih.gov/pubmed/32647259 http://dx.doi.org/10.1038/s41598-020-68357-9 |
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