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The structures of two archaeal type IV pili illuminate evolutionary relationships

We have determined the cryo-electron microscopic (cryo-EM) structures of two archaeal type IV pili (T4P), from Pyrobaculum arsenaticum and Saccharolobus solfataricus, at 3.8 Å and 3.4 Å resolution, respectively. This triples the number of high resolution archaeal T4P structures, and allows us to pin...

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Autores principales: Wang, Fengbin, Baquero, Diana P., Su, Zhangli, Beltran, Leticia C., Prangishvili, David, Krupovic, Mart, Egelman, Edward H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347861/
https://www.ncbi.nlm.nih.gov/pubmed/32647180
http://dx.doi.org/10.1038/s41467-020-17268-4
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author Wang, Fengbin
Baquero, Diana P.
Su, Zhangli
Beltran, Leticia C.
Prangishvili, David
Krupovic, Mart
Egelman, Edward H.
author_facet Wang, Fengbin
Baquero, Diana P.
Su, Zhangli
Beltran, Leticia C.
Prangishvili, David
Krupovic, Mart
Egelman, Edward H.
author_sort Wang, Fengbin
collection PubMed
description We have determined the cryo-electron microscopic (cryo-EM) structures of two archaeal type IV pili (T4P), from Pyrobaculum arsenaticum and Saccharolobus solfataricus, at 3.8 Å and 3.4 Å resolution, respectively. This triples the number of high resolution archaeal T4P structures, and allows us to pinpoint the evolutionary divergence of bacterial T4P, archaeal T4P and archaeal flagellar filaments. We suggest that extensive glycosylation previously observed in T4P of Sulfolobus islandicus is a response to an acidic environment, as at even higher temperatures in a neutral environment much less glycosylation is present for Pyrobaculum than for Sulfolobus and Saccharolobus pili. Consequently, the Pyrobaculum filaments do not display the remarkable stability of the Sulfolobus filaments in vitro. We identify the Saccharolobus and Pyrobaculum T4P as host receptors recognized by rudivirus SSRV1 and tristromavirus PFV2, respectively. Our results illuminate the evolutionary relationships among bacterial and archaeal T4P filaments and provide insights into archaeal virus-host interactions.
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spelling pubmed-73478612020-07-13 The structures of two archaeal type IV pili illuminate evolutionary relationships Wang, Fengbin Baquero, Diana P. Su, Zhangli Beltran, Leticia C. Prangishvili, David Krupovic, Mart Egelman, Edward H. Nat Commun Article We have determined the cryo-electron microscopic (cryo-EM) structures of two archaeal type IV pili (T4P), from Pyrobaculum arsenaticum and Saccharolobus solfataricus, at 3.8 Å and 3.4 Å resolution, respectively. This triples the number of high resolution archaeal T4P structures, and allows us to pinpoint the evolutionary divergence of bacterial T4P, archaeal T4P and archaeal flagellar filaments. We suggest that extensive glycosylation previously observed in T4P of Sulfolobus islandicus is a response to an acidic environment, as at even higher temperatures in a neutral environment much less glycosylation is present for Pyrobaculum than for Sulfolobus and Saccharolobus pili. Consequently, the Pyrobaculum filaments do not display the remarkable stability of the Sulfolobus filaments in vitro. We identify the Saccharolobus and Pyrobaculum T4P as host receptors recognized by rudivirus SSRV1 and tristromavirus PFV2, respectively. Our results illuminate the evolutionary relationships among bacterial and archaeal T4P filaments and provide insights into archaeal virus-host interactions. Nature Publishing Group UK 2020-07-09 /pmc/articles/PMC7347861/ /pubmed/32647180 http://dx.doi.org/10.1038/s41467-020-17268-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Fengbin
Baquero, Diana P.
Su, Zhangli
Beltran, Leticia C.
Prangishvili, David
Krupovic, Mart
Egelman, Edward H.
The structures of two archaeal type IV pili illuminate evolutionary relationships
title The structures of two archaeal type IV pili illuminate evolutionary relationships
title_full The structures of two archaeal type IV pili illuminate evolutionary relationships
title_fullStr The structures of two archaeal type IV pili illuminate evolutionary relationships
title_full_unstemmed The structures of two archaeal type IV pili illuminate evolutionary relationships
title_short The structures of two archaeal type IV pili illuminate evolutionary relationships
title_sort structures of two archaeal type iv pili illuminate evolutionary relationships
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347861/
https://www.ncbi.nlm.nih.gov/pubmed/32647180
http://dx.doi.org/10.1038/s41467-020-17268-4
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