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Discovery of Highly Active Recombinant PNGase H(+) Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes

Peptide-N(4)-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins. So far, only a limited number of PNGase candidates are available for the structural analysis of glycoproteins and their glycan moieties....

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Detalles Bibliográficos
Autores principales:	Guo, Rui-Rui, Comamala, Gerard, Yang, Huan-Huan, Gramlich, Marius, Du, Ya-Min, Wang, Ting, Zeck, Anne, Rand, Kasper Dyrberg, Liu, Li, Voglmeir, Josef
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348039/ https://www.ncbi.nlm.nih.gov/pubmed/32719787 http://dx.doi.org/10.3389/fbioe.2020.00741

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