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Tumour‐derived exosomal miR‐3473b promotes lung tumour cell intrapulmonary colonization by activating the nuclear factor‐κB of local fibroblasts
Tumour‐derived exosomes have been shown to induce pre‐metastatic niche formation, favoring metastatic colonization of tumour cells, but the underlying molecular mechanism is still not fully understood. In this study, we showed that exosomes derived from the LLC cells could indeed significantly enhan...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348150/ https://www.ncbi.nlm.nih.gov/pubmed/32449597 http://dx.doi.org/10.1111/jcmm.15411 |
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author | Du, Cancan Duan, Xixi Yao, Xiaohan Wan, Jiajia Cheng, Yanru Wang, Yuan Yan, Yan Zhang, Lijing Zhu, Linyu Ni, Chen Wang, Ming Qin, Zhihai |
author_facet | Du, Cancan Duan, Xixi Yao, Xiaohan Wan, Jiajia Cheng, Yanru Wang, Yuan Yan, Yan Zhang, Lijing Zhu, Linyu Ni, Chen Wang, Ming Qin, Zhihai |
author_sort | Du, Cancan |
collection | PubMed |
description | Tumour‐derived exosomes have been shown to induce pre‐metastatic niche formation, favoring metastatic colonization of tumour cells, but the underlying molecular mechanism is still not fully understood. In this study, we showed that exosomes derived from the LLC cells could indeed significantly enhance their intrapulmonary colonization. Circulating LLC‐derived exosomes were mainly engulfed by lung fibroblasts and led to the NF‐κB signalling activation. Further studies indicated that the exosomal miR‐3473b was responsible for that by hindering the NFKB inhibitor delta's (NFKBID) function. Blocking miR‐3473b could reverse the exosome‐mediated NF‐κB activation of fibroblasts and decrease intrapulmonary colonization of lung tumour cells. Together, this study demonstrated that the miR‐3473b in exosomes could mediate the interaction of lung tumour cells and local fibroblasts in metastatic sites and, therefore, enhance the metastasis of lung tumour cells. |
format | Online Article Text |
id | pubmed-7348150 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73481502020-07-14 Tumour‐derived exosomal miR‐3473b promotes lung tumour cell intrapulmonary colonization by activating the nuclear factor‐κB of local fibroblasts Du, Cancan Duan, Xixi Yao, Xiaohan Wan, Jiajia Cheng, Yanru Wang, Yuan Yan, Yan Zhang, Lijing Zhu, Linyu Ni, Chen Wang, Ming Qin, Zhihai J Cell Mol Med Original Articles Tumour‐derived exosomes have been shown to induce pre‐metastatic niche formation, favoring metastatic colonization of tumour cells, but the underlying molecular mechanism is still not fully understood. In this study, we showed that exosomes derived from the LLC cells could indeed significantly enhance their intrapulmonary colonization. Circulating LLC‐derived exosomes were mainly engulfed by lung fibroblasts and led to the NF‐κB signalling activation. Further studies indicated that the exosomal miR‐3473b was responsible for that by hindering the NFKB inhibitor delta's (NFKBID) function. Blocking miR‐3473b could reverse the exosome‐mediated NF‐κB activation of fibroblasts and decrease intrapulmonary colonization of lung tumour cells. Together, this study demonstrated that the miR‐3473b in exosomes could mediate the interaction of lung tumour cells and local fibroblasts in metastatic sites and, therefore, enhance the metastasis of lung tumour cells. John Wiley and Sons Inc. 2020-05-25 2020-07 /pmc/articles/PMC7348150/ /pubmed/32449597 http://dx.doi.org/10.1111/jcmm.15411 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Du, Cancan Duan, Xixi Yao, Xiaohan Wan, Jiajia Cheng, Yanru Wang, Yuan Yan, Yan Zhang, Lijing Zhu, Linyu Ni, Chen Wang, Ming Qin, Zhihai Tumour‐derived exosomal miR‐3473b promotes lung tumour cell intrapulmonary colonization by activating the nuclear factor‐κB of local fibroblasts |
title | Tumour‐derived exosomal miR‐3473b promotes lung tumour cell intrapulmonary colonization by activating the nuclear factor‐κB of local fibroblasts |
title_full | Tumour‐derived exosomal miR‐3473b promotes lung tumour cell intrapulmonary colonization by activating the nuclear factor‐κB of local fibroblasts |
title_fullStr | Tumour‐derived exosomal miR‐3473b promotes lung tumour cell intrapulmonary colonization by activating the nuclear factor‐κB of local fibroblasts |
title_full_unstemmed | Tumour‐derived exosomal miR‐3473b promotes lung tumour cell intrapulmonary colonization by activating the nuclear factor‐κB of local fibroblasts |
title_short | Tumour‐derived exosomal miR‐3473b promotes lung tumour cell intrapulmonary colonization by activating the nuclear factor‐κB of local fibroblasts |
title_sort | tumour‐derived exosomal mir‐3473b promotes lung tumour cell intrapulmonary colonization by activating the nuclear factor‐κb of local fibroblasts |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348150/ https://www.ncbi.nlm.nih.gov/pubmed/32449597 http://dx.doi.org/10.1111/jcmm.15411 |
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