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苯乙肼对Molt-4细胞增殖、凋亡及组蛋白甲基化、乙酰化的影响
OBJECTIVE: To investigate the effect of monoamine oxidase inhibitor phenelzine on proliferation, apoptosis and histone modulation in acute lymphoblastic leukemia cell line Molt-4 cells. METHODS: The effect of Phenelzine on cell proliferation was detected by MTT. Apoptotic rate was measured by flow c...
Formato: | Online Artículo Texto |
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Lenguaje: | English |
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Editorial office of Chinese Journal of Hematology
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348193/ https://www.ncbi.nlm.nih.gov/pubmed/27014985 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2016.02.012 |
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collection | PubMed |
description | OBJECTIVE: To investigate the effect of monoamine oxidase inhibitor phenelzine on proliferation, apoptosis and histone modulation in acute lymphoblastic leukemia cell line Molt-4 cells. METHODS: The effect of Phenelzine on cell proliferation was detected by MTT. Apoptotic rate was measured by flow cytometry. The variation of apoptosis associated proteins Caspase-3, Bcl-2 and Bax, cyclin-dependent kinase inhibitor p21, tumor suppressor protein p15, and the expression level of histone methylation of H3K4, H3K9 and histone acetylation of H3, DNMT1 were detected by Western Blot. RESULTS: ①Molt-4 cell proliferation rates were (87.68±3.54)%, (67.84±3.24)%, (51.48±3.37)%, (28.72±2.56)% respectively after exposured to phenelzine at 5, 10, 15, 20 µmol/L for 24 h,P<0.05. ②After 10 µmol/L of phenelzine exposure for 24, 48, 72 h, cell proliferation rates were (67.84±3.24)%, (50.24±2.01)%, (40.31±2.25)%,P<0.05. ③The apoptotic rates were (13.64±2.58)%, (31.24±3.42)%, (56.37±4.26)% after phenelzine treatment at 5, 10, 20 µmol/L for 24 h, which was concentration dependent. ④Phenelzine could upregulate the expression of Bax, caspase-3, p21, and downregulate Bcl-2 expression. Phenelzine upregulated the methylation level of histone H3K4me1, H3K4me2 and histone acetylated H3, while it didn't change the level of histone H3K4me3, H3K9me1, H3K9me2. ⑤Phenelzine inhibited DNMT1 expression and promoted p15 expression. CONCLUSION: Phenelzine increased the methylation of histone H3K4me1, H3K4me2, acetylation of histone H3 and p21, and decreased the expression of DNMT1 and p15, and ultimately inhibited the proliferation and apoptosis of Molt-4 cells. |
format | Online Article Text |
id | pubmed-7348193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Editorial office of Chinese Journal of Hematology |
record_format | MEDLINE/PubMed |
spelling | pubmed-73481932020-07-16 苯乙肼对Molt-4细胞增殖、凋亡及组蛋白甲基化、乙酰化的影响 Zhonghua Xue Ye Xue Za Zhi 论著 OBJECTIVE: To investigate the effect of monoamine oxidase inhibitor phenelzine on proliferation, apoptosis and histone modulation in acute lymphoblastic leukemia cell line Molt-4 cells. METHODS: The effect of Phenelzine on cell proliferation was detected by MTT. Apoptotic rate was measured by flow cytometry. The variation of apoptosis associated proteins Caspase-3, Bcl-2 and Bax, cyclin-dependent kinase inhibitor p21, tumor suppressor protein p15, and the expression level of histone methylation of H3K4, H3K9 and histone acetylation of H3, DNMT1 were detected by Western Blot. RESULTS: ①Molt-4 cell proliferation rates were (87.68±3.54)%, (67.84±3.24)%, (51.48±3.37)%, (28.72±2.56)% respectively after exposured to phenelzine at 5, 10, 15, 20 µmol/L for 24 h,P<0.05. ②After 10 µmol/L of phenelzine exposure for 24, 48, 72 h, cell proliferation rates were (67.84±3.24)%, (50.24±2.01)%, (40.31±2.25)%,P<0.05. ③The apoptotic rates were (13.64±2.58)%, (31.24±3.42)%, (56.37±4.26)% after phenelzine treatment at 5, 10, 20 µmol/L for 24 h, which was concentration dependent. ④Phenelzine could upregulate the expression of Bax, caspase-3, p21, and downregulate Bcl-2 expression. Phenelzine upregulated the methylation level of histone H3K4me1, H3K4me2 and histone acetylated H3, while it didn't change the level of histone H3K4me3, H3K9me1, H3K9me2. ⑤Phenelzine inhibited DNMT1 expression and promoted p15 expression. CONCLUSION: Phenelzine increased the methylation of histone H3K4me1, H3K4me2, acetylation of histone H3 and p21, and decreased the expression of DNMT1 and p15, and ultimately inhibited the proliferation and apoptosis of Molt-4 cells. Editorial office of Chinese Journal of Hematology 2016-02 /pmc/articles/PMC7348193/ /pubmed/27014985 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2016.02.012 Text en 2016年版权归中华医学会所有 http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution 3.0 License (CC-BY-NC). The Copyright own by Publisher. Without authorization, shall not reprint, except this publication article, shall not use this publication format design. Unless otherwise stated, all articles published in this journal do not represent the views of the Chinese Medical Association or the editorial board of this journal. |
spellingShingle | 论著 苯乙肼对Molt-4细胞增殖、凋亡及组蛋白甲基化、乙酰化的影响 |
title | 苯乙肼对Molt-4细胞增殖、凋亡及组蛋白甲基化、乙酰化的影响 |
title_full | 苯乙肼对Molt-4细胞增殖、凋亡及组蛋白甲基化、乙酰化的影响 |
title_fullStr | 苯乙肼对Molt-4细胞增殖、凋亡及组蛋白甲基化、乙酰化的影响 |
title_full_unstemmed | 苯乙肼对Molt-4细胞增殖、凋亡及组蛋白甲基化、乙酰化的影响 |
title_short | 苯乙肼对Molt-4细胞增殖、凋亡及组蛋白甲基化、乙酰化的影响 |
title_sort | 苯乙肼对molt-4细胞增殖、凋亡及组蛋白甲基化、乙酰化的影响 |
topic | 论著 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348193/ https://www.ncbi.nlm.nih.gov/pubmed/27014985 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2016.02.012 |
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