Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean

RNA editing plays a key role in organelle gene expression. Little is known about how RNA editing factors influence soybean plant development. Here, we report the isolation and characterization of a soybean yl (yellow leaf) mutant. The yl plants showed decreased chlorophyll accumulation, lower PS II...

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Autores principales: Zhu, Xiaowei, Pan, Yi, Liu, Zhi, Liu, Yucheng, Zhong, Deyi, Duan, Zongbiao, Tian, Zhixi, Zhu, Baoge, Zhou, Guoan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348699/
https://www.ncbi.nlm.nih.gov/pubmed/32560081
http://dx.doi.org/10.3390/ijms21124275
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author Zhu, Xiaowei
Pan, Yi
Liu, Zhi
Liu, Yucheng
Zhong, Deyi
Duan, Zongbiao
Tian, Zhixi
Zhu, Baoge
Zhou, Guoan
author_facet Zhu, Xiaowei
Pan, Yi
Liu, Zhi
Liu, Yucheng
Zhong, Deyi
Duan, Zongbiao
Tian, Zhixi
Zhu, Baoge
Zhou, Guoan
author_sort Zhu, Xiaowei
collection PubMed
description RNA editing plays a key role in organelle gene expression. Little is known about how RNA editing factors influence soybean plant development. Here, we report the isolation and characterization of a soybean yl (yellow leaf) mutant. The yl plants showed decreased chlorophyll accumulation, lower PS II activity, an impaired net photosynthesis rate, and an altered chloroplast ultrastructure. Fine mapping of YL uncovered a point mutation in Glyma.20G187000, which encodes a chloroplast-localized protein homologous to Arabidopsis thaliana (Arabidopsis) ORRM1. YL is mainly expressed in trifoliate leaves, and its deficiency affects the editing of multiple chloroplast RNA sites, leading to inferior photosynthesis in soybean. Taken together, these results demonstrate the importance of the soybean YL protein in chloroplast RNA editing and photosynthesis.
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spelling pubmed-73486992020-07-15 Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean Zhu, Xiaowei Pan, Yi Liu, Zhi Liu, Yucheng Zhong, Deyi Duan, Zongbiao Tian, Zhixi Zhu, Baoge Zhou, Guoan Int J Mol Sci Article RNA editing plays a key role in organelle gene expression. Little is known about how RNA editing factors influence soybean plant development. Here, we report the isolation and characterization of a soybean yl (yellow leaf) mutant. The yl plants showed decreased chlorophyll accumulation, lower PS II activity, an impaired net photosynthesis rate, and an altered chloroplast ultrastructure. Fine mapping of YL uncovered a point mutation in Glyma.20G187000, which encodes a chloroplast-localized protein homologous to Arabidopsis thaliana (Arabidopsis) ORRM1. YL is mainly expressed in trifoliate leaves, and its deficiency affects the editing of multiple chloroplast RNA sites, leading to inferior photosynthesis in soybean. Taken together, these results demonstrate the importance of the soybean YL protein in chloroplast RNA editing and photosynthesis. MDPI 2020-06-16 /pmc/articles/PMC7348699/ /pubmed/32560081 http://dx.doi.org/10.3390/ijms21124275 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhu, Xiaowei
Pan, Yi
Liu, Zhi
Liu, Yucheng
Zhong, Deyi
Duan, Zongbiao
Tian, Zhixi
Zhu, Baoge
Zhou, Guoan
Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean
title Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean
title_full Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean
title_fullStr Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean
title_full_unstemmed Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean
title_short Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean
title_sort mutation of yl results in a yellow leaf with chloroplast rna editing defect in soybean
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348699/
https://www.ncbi.nlm.nih.gov/pubmed/32560081
http://dx.doi.org/10.3390/ijms21124275
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