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Identification of Molecular Mechanisms Related to Pig Fatness at the Transcriptome and miRNAome Levels

Fat deposition and growth rate are closely related to pork quality and fattening efficiency. The next-generation sequencing (NGS) approach for transcriptome and miRNAome massive parallel sequencing of adipocyte tissue was applied to search for a molecular network related to fat deposition in pigs. P...

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Autores principales: Ropka-Molik, Katarzyna, Pawlina-Tyszko, Klaudia, Żukowski, Kacper, Tyra, Mirosław, Derebecka, Natalia, Wesoły, Joanna, Szmatoła, Tomasz, Piórkowska, Katarzyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348756/
https://www.ncbi.nlm.nih.gov/pubmed/32485856
http://dx.doi.org/10.3390/genes11060600
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author Ropka-Molik, Katarzyna
Pawlina-Tyszko, Klaudia
Żukowski, Kacper
Tyra, Mirosław
Derebecka, Natalia
Wesoły, Joanna
Szmatoła, Tomasz
Piórkowska, Katarzyna
author_facet Ropka-Molik, Katarzyna
Pawlina-Tyszko, Klaudia
Żukowski, Kacper
Tyra, Mirosław
Derebecka, Natalia
Wesoły, Joanna
Szmatoła, Tomasz
Piórkowska, Katarzyna
author_sort Ropka-Molik, Katarzyna
collection PubMed
description Fat deposition and growth rate are closely related to pork quality and fattening efficiency. The next-generation sequencing (NGS) approach for transcriptome and miRNAome massive parallel sequencing of adipocyte tissue was applied to search for a molecular network related to fat deposition in pigs. Pigs were represented by three breeds (Large White, Pietrain, and Hampshire) that varied in fat content within each breed. The obtained results allowed for the detection of significant enrichment of Gene Ontology (GO) terms and pathways associated directly and indirectly with fat deposition via regulation of fatty acid metabolism, fat cell differentiation, inflammatory response, and extracellular matrix (ECM) organization and disassembly. Moreover, the results showed that adipocyte tissue content strongly affected the expression of leptin and other genes related to a response to excessive feed intake. The findings indicated that modification of genes and miRNAs involved in ECM rearrangements can be essential during fat tissue growth and development in pigs. The identified molecular network within genes and miRNAs that were deregulated depending on the subcutaneous fat level are proposed as candidate factors determining adipogenesis, fatness, and selected fattening characteristics in pigs.
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spelling pubmed-73487562020-07-20 Identification of Molecular Mechanisms Related to Pig Fatness at the Transcriptome and miRNAome Levels Ropka-Molik, Katarzyna Pawlina-Tyszko, Klaudia Żukowski, Kacper Tyra, Mirosław Derebecka, Natalia Wesoły, Joanna Szmatoła, Tomasz Piórkowska, Katarzyna Genes (Basel) Article Fat deposition and growth rate are closely related to pork quality and fattening efficiency. The next-generation sequencing (NGS) approach for transcriptome and miRNAome massive parallel sequencing of adipocyte tissue was applied to search for a molecular network related to fat deposition in pigs. Pigs were represented by three breeds (Large White, Pietrain, and Hampshire) that varied in fat content within each breed. The obtained results allowed for the detection of significant enrichment of Gene Ontology (GO) terms and pathways associated directly and indirectly with fat deposition via regulation of fatty acid metabolism, fat cell differentiation, inflammatory response, and extracellular matrix (ECM) organization and disassembly. Moreover, the results showed that adipocyte tissue content strongly affected the expression of leptin and other genes related to a response to excessive feed intake. The findings indicated that modification of genes and miRNAs involved in ECM rearrangements can be essential during fat tissue growth and development in pigs. The identified molecular network within genes and miRNAs that were deregulated depending on the subcutaneous fat level are proposed as candidate factors determining adipogenesis, fatness, and selected fattening characteristics in pigs. MDPI 2020-05-29 /pmc/articles/PMC7348756/ /pubmed/32485856 http://dx.doi.org/10.3390/genes11060600 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ropka-Molik, Katarzyna
Pawlina-Tyszko, Klaudia
Żukowski, Kacper
Tyra, Mirosław
Derebecka, Natalia
Wesoły, Joanna
Szmatoła, Tomasz
Piórkowska, Katarzyna
Identification of Molecular Mechanisms Related to Pig Fatness at the Transcriptome and miRNAome Levels
title Identification of Molecular Mechanisms Related to Pig Fatness at the Transcriptome and miRNAome Levels
title_full Identification of Molecular Mechanisms Related to Pig Fatness at the Transcriptome and miRNAome Levels
title_fullStr Identification of Molecular Mechanisms Related to Pig Fatness at the Transcriptome and miRNAome Levels
title_full_unstemmed Identification of Molecular Mechanisms Related to Pig Fatness at the Transcriptome and miRNAome Levels
title_short Identification of Molecular Mechanisms Related to Pig Fatness at the Transcriptome and miRNAome Levels
title_sort identification of molecular mechanisms related to pig fatness at the transcriptome and mirnaome levels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348756/
https://www.ncbi.nlm.nih.gov/pubmed/32485856
http://dx.doi.org/10.3390/genes11060600
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