Cargando…

Antibiotic Resistance Is Associated with Integrative and Conjugative Elements and Genomic Islands in Naturally Circulating Streptococcus pneumoniae Isolates from Adults in Liverpool, UK

Pneumonia is the sixth largest cause of death in the UK. It is usually caused by Streptococcus pneumoniae, which healthy individuals can carry in their nose without symptoms of disease. Antimicrobial resistance further increases mortality and morbidity associated with pneumococcal infection, althoug...

Descripción completa

Detalles Bibliográficos
Autores principales: Nikolaou, Elissavet, Hubbard, Alasdair T. M., Botelho, João, Marschall, Taylor A. M., Ferreira, Daniela M., Roberts, Adam P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348760/
https://www.ncbi.nlm.nih.gov/pubmed/32517221
http://dx.doi.org/10.3390/genes11060625
_version_ 1783556904532836352
author Nikolaou, Elissavet
Hubbard, Alasdair T. M.
Botelho, João
Marschall, Taylor A. M.
Ferreira, Daniela M.
Roberts, Adam P.
author_facet Nikolaou, Elissavet
Hubbard, Alasdair T. M.
Botelho, João
Marschall, Taylor A. M.
Ferreira, Daniela M.
Roberts, Adam P.
author_sort Nikolaou, Elissavet
collection PubMed
description Pneumonia is the sixth largest cause of death in the UK. It is usually caused by Streptococcus pneumoniae, which healthy individuals can carry in their nose without symptoms of disease. Antimicrobial resistance further increases mortality and morbidity associated with pneumococcal infection, although few studies have analysed resistance in naturally circulating pneumococcal isolates in adult populations. Here, we report on the resistome and associated mobile genetic elements within circulating pneumococcus isolated from adult volunteers enrolled in the experimental human pneumococcal colonisation (EHPC) research program at the Liverpool School of Tropical Medicine, UK. Pneumococcal isolates collected from 30 healthy asymptomatic adults who had volunteered to take part in clinical research were screened for antibiotic susceptibility to erythromycin and tetracycline, and whole-genome sequenced. The genetic context of resistance to one or both antibiotics in four isolates was characterised bioinformatically, and any association of the resistance genes with mobile genetic elements was determined. Tetracycline and macrolide resistance genes [tet(M), erm(B), mef(A), msr(D)] were detected on known Tn916-like integrative and conjugative elements, namely Tn6002 and Tn2010, and tet(32) was found for the first time in S. pneumoniae located on a novel 50 kb genomic island. The widespread use of pneumococcal conjugate vaccines impacts on serotype prevalence and transmission within the community. It is therefore important to continue to monitor antimicrobial resistance (AMR) genes present in both vaccine types and non-vaccine types in response to contemporary antimicrobial therapies and characterise the genetic context of acquired resistance genes to continually optimise antibiotic therapies.
format Online
Article
Text
id pubmed-7348760
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73487602020-07-20 Antibiotic Resistance Is Associated with Integrative and Conjugative Elements and Genomic Islands in Naturally Circulating Streptococcus pneumoniae Isolates from Adults in Liverpool, UK Nikolaou, Elissavet Hubbard, Alasdair T. M. Botelho, João Marschall, Taylor A. M. Ferreira, Daniela M. Roberts, Adam P. Genes (Basel) Communication Pneumonia is the sixth largest cause of death in the UK. It is usually caused by Streptococcus pneumoniae, which healthy individuals can carry in their nose without symptoms of disease. Antimicrobial resistance further increases mortality and morbidity associated with pneumococcal infection, although few studies have analysed resistance in naturally circulating pneumococcal isolates in adult populations. Here, we report on the resistome and associated mobile genetic elements within circulating pneumococcus isolated from adult volunteers enrolled in the experimental human pneumococcal colonisation (EHPC) research program at the Liverpool School of Tropical Medicine, UK. Pneumococcal isolates collected from 30 healthy asymptomatic adults who had volunteered to take part in clinical research were screened for antibiotic susceptibility to erythromycin and tetracycline, and whole-genome sequenced. The genetic context of resistance to one or both antibiotics in four isolates was characterised bioinformatically, and any association of the resistance genes with mobile genetic elements was determined. Tetracycline and macrolide resistance genes [tet(M), erm(B), mef(A), msr(D)] were detected on known Tn916-like integrative and conjugative elements, namely Tn6002 and Tn2010, and tet(32) was found for the first time in S. pneumoniae located on a novel 50 kb genomic island. The widespread use of pneumococcal conjugate vaccines impacts on serotype prevalence and transmission within the community. It is therefore important to continue to monitor antimicrobial resistance (AMR) genes present in both vaccine types and non-vaccine types in response to contemporary antimicrobial therapies and characterise the genetic context of acquired resistance genes to continually optimise antibiotic therapies. MDPI 2020-06-06 /pmc/articles/PMC7348760/ /pubmed/32517221 http://dx.doi.org/10.3390/genes11060625 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Nikolaou, Elissavet
Hubbard, Alasdair T. M.
Botelho, João
Marschall, Taylor A. M.
Ferreira, Daniela M.
Roberts, Adam P.
Antibiotic Resistance Is Associated with Integrative and Conjugative Elements and Genomic Islands in Naturally Circulating Streptococcus pneumoniae Isolates from Adults in Liverpool, UK
title Antibiotic Resistance Is Associated with Integrative and Conjugative Elements and Genomic Islands in Naturally Circulating Streptococcus pneumoniae Isolates from Adults in Liverpool, UK
title_full Antibiotic Resistance Is Associated with Integrative and Conjugative Elements and Genomic Islands in Naturally Circulating Streptococcus pneumoniae Isolates from Adults in Liverpool, UK
title_fullStr Antibiotic Resistance Is Associated with Integrative and Conjugative Elements and Genomic Islands in Naturally Circulating Streptococcus pneumoniae Isolates from Adults in Liverpool, UK
title_full_unstemmed Antibiotic Resistance Is Associated with Integrative and Conjugative Elements and Genomic Islands in Naturally Circulating Streptococcus pneumoniae Isolates from Adults in Liverpool, UK
title_short Antibiotic Resistance Is Associated with Integrative and Conjugative Elements and Genomic Islands in Naturally Circulating Streptococcus pneumoniae Isolates from Adults in Liverpool, UK
title_sort antibiotic resistance is associated with integrative and conjugative elements and genomic islands in naturally circulating streptococcus pneumoniae isolates from adults in liverpool, uk
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348760/
https://www.ncbi.nlm.nih.gov/pubmed/32517221
http://dx.doi.org/10.3390/genes11060625
work_keys_str_mv AT nikolaouelissavet antibioticresistanceisassociatedwithintegrativeandconjugativeelementsandgenomicislandsinnaturallycirculatingstreptococcuspneumoniaeisolatesfromadultsinliverpooluk
AT hubbardalasdairtm antibioticresistanceisassociatedwithintegrativeandconjugativeelementsandgenomicislandsinnaturallycirculatingstreptococcuspneumoniaeisolatesfromadultsinliverpooluk
AT botelhojoao antibioticresistanceisassociatedwithintegrativeandconjugativeelementsandgenomicislandsinnaturallycirculatingstreptococcuspneumoniaeisolatesfromadultsinliverpooluk
AT marschalltayloram antibioticresistanceisassociatedwithintegrativeandconjugativeelementsandgenomicislandsinnaturallycirculatingstreptococcuspneumoniaeisolatesfromadultsinliverpooluk
AT ferreiradanielam antibioticresistanceisassociatedwithintegrativeandconjugativeelementsandgenomicislandsinnaturallycirculatingstreptococcuspneumoniaeisolatesfromadultsinliverpooluk
AT robertsadamp antibioticresistanceisassociatedwithintegrativeandconjugativeelementsandgenomicislandsinnaturallycirculatingstreptococcuspneumoniaeisolatesfromadultsinliverpooluk