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Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats

Satellite DNAs are tandemly repeated sequences preferentially assembled into large arrays within constitutive heterochromatin and their transcription is often activated by stress conditions, particularly by heat stress. Bioinformatic analyses of sequenced genomes however reveal single repeats or sho...

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Autores principales: Feliciello, Isidoro, Sermek, Antonio, Pezer, Željka, Matulić, Maja, Ugarković, Đurđica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348797/
https://www.ncbi.nlm.nih.gov/pubmed/32570830
http://dx.doi.org/10.3390/genes11060663
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author Feliciello, Isidoro
Sermek, Antonio
Pezer, Željka
Matulić, Maja
Ugarković, Đurđica
author_facet Feliciello, Isidoro
Sermek, Antonio
Pezer, Željka
Matulić, Maja
Ugarković, Đurđica
author_sort Feliciello, Isidoro
collection PubMed
description Satellite DNAs are tandemly repeated sequences preferentially assembled into large arrays within constitutive heterochromatin and their transcription is often activated by stress conditions, particularly by heat stress. Bioinformatic analyses of sequenced genomes however reveal single repeats or short arrays of satellite DNAs dispersed in the vicinity of genes within euchromatin. Here, we analyze transcription of a major human alpha satellite DNA upon heat stress and follow the dynamics of “silent” H3K9me3 and “active” H3K4me2/3 histone marks at dispersed euchromatic and tandemly arranged heterochromatic alpha repeats. The results show H3K9me3 enrichment at alpha repeats upon heat stress, which correlates with the dynamics of alpha satellite DNA transcription activation, while no change in H3K4me2/3 level is detected. Spreading of H3K9me3 up to 1–2 kb from the insertion sites of the euchromatic alpha repeats is detected, revealing the alpha repeats as modulators of local chromatin structure. In addition, expression of genes containing alpha repeats within introns as well as of genes closest to the intergenic alpha repeats is downregulated upon heat stress. Further studies are necessary to reveal the possible contribution of H3K9me3 enriched alpha repeats, in particular those located within introns, to the silencing of their associated genes.
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spelling pubmed-73487972020-07-20 Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats Feliciello, Isidoro Sermek, Antonio Pezer, Željka Matulić, Maja Ugarković, Đurđica Genes (Basel) Article Satellite DNAs are tandemly repeated sequences preferentially assembled into large arrays within constitutive heterochromatin and their transcription is often activated by stress conditions, particularly by heat stress. Bioinformatic analyses of sequenced genomes however reveal single repeats or short arrays of satellite DNAs dispersed in the vicinity of genes within euchromatin. Here, we analyze transcription of a major human alpha satellite DNA upon heat stress and follow the dynamics of “silent” H3K9me3 and “active” H3K4me2/3 histone marks at dispersed euchromatic and tandemly arranged heterochromatic alpha repeats. The results show H3K9me3 enrichment at alpha repeats upon heat stress, which correlates with the dynamics of alpha satellite DNA transcription activation, while no change in H3K4me2/3 level is detected. Spreading of H3K9me3 up to 1–2 kb from the insertion sites of the euchromatic alpha repeats is detected, revealing the alpha repeats as modulators of local chromatin structure. In addition, expression of genes containing alpha repeats within introns as well as of genes closest to the intergenic alpha repeats is downregulated upon heat stress. Further studies are necessary to reveal the possible contribution of H3K9me3 enriched alpha repeats, in particular those located within introns, to the silencing of their associated genes. MDPI 2020-06-18 /pmc/articles/PMC7348797/ /pubmed/32570830 http://dx.doi.org/10.3390/genes11060663 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Feliciello, Isidoro
Sermek, Antonio
Pezer, Željka
Matulić, Maja
Ugarković, Đurđica
Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats
title Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats
title_full Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats
title_fullStr Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats
title_full_unstemmed Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats
title_short Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats
title_sort heat stress affects h3k9me3 level at human alpha satellite dna repeats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348797/
https://www.ncbi.nlm.nih.gov/pubmed/32570830
http://dx.doi.org/10.3390/genes11060663
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