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Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats
Satellite DNAs are tandemly repeated sequences preferentially assembled into large arrays within constitutive heterochromatin and their transcription is often activated by stress conditions, particularly by heat stress. Bioinformatic analyses of sequenced genomes however reveal single repeats or sho...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348797/ https://www.ncbi.nlm.nih.gov/pubmed/32570830 http://dx.doi.org/10.3390/genes11060663 |
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author | Feliciello, Isidoro Sermek, Antonio Pezer, Željka Matulić, Maja Ugarković, Đurđica |
author_facet | Feliciello, Isidoro Sermek, Antonio Pezer, Željka Matulić, Maja Ugarković, Đurđica |
author_sort | Feliciello, Isidoro |
collection | PubMed |
description | Satellite DNAs are tandemly repeated sequences preferentially assembled into large arrays within constitutive heterochromatin and their transcription is often activated by stress conditions, particularly by heat stress. Bioinformatic analyses of sequenced genomes however reveal single repeats or short arrays of satellite DNAs dispersed in the vicinity of genes within euchromatin. Here, we analyze transcription of a major human alpha satellite DNA upon heat stress and follow the dynamics of “silent” H3K9me3 and “active” H3K4me2/3 histone marks at dispersed euchromatic and tandemly arranged heterochromatic alpha repeats. The results show H3K9me3 enrichment at alpha repeats upon heat stress, which correlates with the dynamics of alpha satellite DNA transcription activation, while no change in H3K4me2/3 level is detected. Spreading of H3K9me3 up to 1–2 kb from the insertion sites of the euchromatic alpha repeats is detected, revealing the alpha repeats as modulators of local chromatin structure. In addition, expression of genes containing alpha repeats within introns as well as of genes closest to the intergenic alpha repeats is downregulated upon heat stress. Further studies are necessary to reveal the possible contribution of H3K9me3 enriched alpha repeats, in particular those located within introns, to the silencing of their associated genes. |
format | Online Article Text |
id | pubmed-7348797 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73487972020-07-20 Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats Feliciello, Isidoro Sermek, Antonio Pezer, Željka Matulić, Maja Ugarković, Đurđica Genes (Basel) Article Satellite DNAs are tandemly repeated sequences preferentially assembled into large arrays within constitutive heterochromatin and their transcription is often activated by stress conditions, particularly by heat stress. Bioinformatic analyses of sequenced genomes however reveal single repeats or short arrays of satellite DNAs dispersed in the vicinity of genes within euchromatin. Here, we analyze transcription of a major human alpha satellite DNA upon heat stress and follow the dynamics of “silent” H3K9me3 and “active” H3K4me2/3 histone marks at dispersed euchromatic and tandemly arranged heterochromatic alpha repeats. The results show H3K9me3 enrichment at alpha repeats upon heat stress, which correlates with the dynamics of alpha satellite DNA transcription activation, while no change in H3K4me2/3 level is detected. Spreading of H3K9me3 up to 1–2 kb from the insertion sites of the euchromatic alpha repeats is detected, revealing the alpha repeats as modulators of local chromatin structure. In addition, expression of genes containing alpha repeats within introns as well as of genes closest to the intergenic alpha repeats is downregulated upon heat stress. Further studies are necessary to reveal the possible contribution of H3K9me3 enriched alpha repeats, in particular those located within introns, to the silencing of their associated genes. MDPI 2020-06-18 /pmc/articles/PMC7348797/ /pubmed/32570830 http://dx.doi.org/10.3390/genes11060663 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Feliciello, Isidoro Sermek, Antonio Pezer, Željka Matulić, Maja Ugarković, Đurđica Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats |
title | Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats |
title_full | Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats |
title_fullStr | Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats |
title_full_unstemmed | Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats |
title_short | Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats |
title_sort | heat stress affects h3k9me3 level at human alpha satellite dna repeats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348797/ https://www.ncbi.nlm.nih.gov/pubmed/32570830 http://dx.doi.org/10.3390/genes11060663 |
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