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Phenotypic Variability and Genetic Diversity in a Pinus koraiensis Clonal Trial in Northeastern China
Combining phenotypic and genetic characteristics in a genetic variation study is of paramount importance to effectively orient the selection of producers’ elite trees in a seed orchard. In total, 28 phenotypic characteristics and 16 microsatellite loci were used to analyze the clonal genetic variati...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348814/ https://www.ncbi.nlm.nih.gov/pubmed/32575537 http://dx.doi.org/10.3390/genes11060673 |
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author | Kaviriri, David Kombi Zhang, Qinhui Zhang, Xinxin Jiang, Luping Zhang, Jinpeng Wang, Jingyuan Khasa, Damase P. You, Xiangling Zhao, Xiyang |
author_facet | Kaviriri, David Kombi Zhang, Qinhui Zhang, Xinxin Jiang, Luping Zhang, Jinpeng Wang, Jingyuan Khasa, Damase P. You, Xiangling Zhao, Xiyang |
author_sort | Kaviriri, David Kombi |
collection | PubMed |
description | Combining phenotypic and genetic characteristics in a genetic variation study is of paramount importance to effectively orient the selection of producers’ elite trees in a seed orchard. In total, 28 phenotypic characteristics and 16 microsatellite loci were used to analyze the clonal genetic variation, to characterize the genetic diversity, and to refine the genetic classifications of 110 Pinus koraiensis clones grown in the Naozhi orchard in northeastern China. All clones were significantly different in most traits. Most of the phenotypic characteristics showed great genetic variation among clones, while the genotypic differentiation was weak between the selection sites of clones. The SSR markers showed a relatively high level of genetic diversity (Na = 4.67 ± 0.43, Ne = 2.916 ± 0.18, I = 1.15 ± 0.07, Ho = 0.69 ± 0.04, He = 0.62 ± 0.02, and mean polymorphic information content (PIC) of 0.574), with higher heterozygosity as an indication of a lower probability of inbreeding events in the orchard. Despite weak correlation coefficients between dissimilarity matrices (r(A/B), range equal to 0.022, p-value < 0.001), the genetic and phenotypic classifications congruently subdivided all the clones into three major groups. The patterns of phenotypic trait variations and genetic diversity are valuable to effectively select materials in breeding programs of P. koraiensis. |
format | Online Article Text |
id | pubmed-7348814 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73488142020-07-22 Phenotypic Variability and Genetic Diversity in a Pinus koraiensis Clonal Trial in Northeastern China Kaviriri, David Kombi Zhang, Qinhui Zhang, Xinxin Jiang, Luping Zhang, Jinpeng Wang, Jingyuan Khasa, Damase P. You, Xiangling Zhao, Xiyang Genes (Basel) Article Combining phenotypic and genetic characteristics in a genetic variation study is of paramount importance to effectively orient the selection of producers’ elite trees in a seed orchard. In total, 28 phenotypic characteristics and 16 microsatellite loci were used to analyze the clonal genetic variation, to characterize the genetic diversity, and to refine the genetic classifications of 110 Pinus koraiensis clones grown in the Naozhi orchard in northeastern China. All clones were significantly different in most traits. Most of the phenotypic characteristics showed great genetic variation among clones, while the genotypic differentiation was weak between the selection sites of clones. The SSR markers showed a relatively high level of genetic diversity (Na = 4.67 ± 0.43, Ne = 2.916 ± 0.18, I = 1.15 ± 0.07, Ho = 0.69 ± 0.04, He = 0.62 ± 0.02, and mean polymorphic information content (PIC) of 0.574), with higher heterozygosity as an indication of a lower probability of inbreeding events in the orchard. Despite weak correlation coefficients between dissimilarity matrices (r(A/B), range equal to 0.022, p-value < 0.001), the genetic and phenotypic classifications congruently subdivided all the clones into three major groups. The patterns of phenotypic trait variations and genetic diversity are valuable to effectively select materials in breeding programs of P. koraiensis. MDPI 2020-06-19 /pmc/articles/PMC7348814/ /pubmed/32575537 http://dx.doi.org/10.3390/genes11060673 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kaviriri, David Kombi Zhang, Qinhui Zhang, Xinxin Jiang, Luping Zhang, Jinpeng Wang, Jingyuan Khasa, Damase P. You, Xiangling Zhao, Xiyang Phenotypic Variability and Genetic Diversity in a Pinus koraiensis Clonal Trial in Northeastern China |
title | Phenotypic Variability and Genetic Diversity in a Pinus koraiensis Clonal Trial in Northeastern China |
title_full | Phenotypic Variability and Genetic Diversity in a Pinus koraiensis Clonal Trial in Northeastern China |
title_fullStr | Phenotypic Variability and Genetic Diversity in a Pinus koraiensis Clonal Trial in Northeastern China |
title_full_unstemmed | Phenotypic Variability and Genetic Diversity in a Pinus koraiensis Clonal Trial in Northeastern China |
title_short | Phenotypic Variability and Genetic Diversity in a Pinus koraiensis Clonal Trial in Northeastern China |
title_sort | phenotypic variability and genetic diversity in a pinus koraiensis clonal trial in northeastern china |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348814/ https://www.ncbi.nlm.nih.gov/pubmed/32575537 http://dx.doi.org/10.3390/genes11060673 |
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