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Contribution of Dopamine Transporter Gene Methylation Status to Cannabis Dependency

The susceptibility to cannabis dependency results from the influence of numerous factors such as social, genetic, as well as epigenetic factors. Many studies have attempted to discover a molecular basis for this disease. However, our study aimed at evaluating the connection between altered methylati...

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Autores principales: Grzywacz, Anna, Barczak, Wojciech, Chmielowiec, Jolanta, Chmielowiec, Krzysztof, Suchanecka, Aleksandra, Trybek, Grzegorz, Masiak, Jolanta, Jagielski, Paweł, Grocholewicz, Katarzyna, Rubiś, Blazej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348832/
https://www.ncbi.nlm.nih.gov/pubmed/32586035
http://dx.doi.org/10.3390/brainsci10060400
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author Grzywacz, Anna
Barczak, Wojciech
Chmielowiec, Jolanta
Chmielowiec, Krzysztof
Suchanecka, Aleksandra
Trybek, Grzegorz
Masiak, Jolanta
Jagielski, Paweł
Grocholewicz, Katarzyna
Rubiś, Blazej
author_facet Grzywacz, Anna
Barczak, Wojciech
Chmielowiec, Jolanta
Chmielowiec, Krzysztof
Suchanecka, Aleksandra
Trybek, Grzegorz
Masiak, Jolanta
Jagielski, Paweł
Grocholewicz, Katarzyna
Rubiś, Blazej
author_sort Grzywacz, Anna
collection PubMed
description The susceptibility to cannabis dependency results from the influence of numerous factors such as social, genetic, as well as epigenetic factors. Many studies have attempted to discover a molecular basis for this disease. However, our study aimed at evaluating the connection between altered methylation of the dopamine transporter gene (DAT1) promoter CpG sites and cannabis dependency. In the cases of some DNA sequences, including the DAT1 gene region, their methylation status in blood cells may reflect a systemic modulation in the whole organism. Consequently, we isolated the DNA from the peripheral blood cells from a group of 201 cannabis-dependent patients and 285 controls who were healthy volunteers and who were matched for age and sex. The DNA was subjected to bisulfite conversion and sequencing. Our analysis revealed no statistical differences in the general methylation status of the DAT1 gene promoter CpG island between the patients and controls. Yet, the analysis of individual CpG sites where methylation occurred indicated significant differences. These sites are known to be bound by transcription factors (e.g., SP1, p53, PAX5, or GR), which, apart from other functions, were shown to play a role in the development of the nervous system. Therefore, DAT1 gene promoter methylation studies may provide important insight into the mechanism of cannabis dependency.
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spelling pubmed-73488322020-07-22 Contribution of Dopamine Transporter Gene Methylation Status to Cannabis Dependency Grzywacz, Anna Barczak, Wojciech Chmielowiec, Jolanta Chmielowiec, Krzysztof Suchanecka, Aleksandra Trybek, Grzegorz Masiak, Jolanta Jagielski, Paweł Grocholewicz, Katarzyna Rubiś, Blazej Brain Sci Article The susceptibility to cannabis dependency results from the influence of numerous factors such as social, genetic, as well as epigenetic factors. Many studies have attempted to discover a molecular basis for this disease. However, our study aimed at evaluating the connection between altered methylation of the dopamine transporter gene (DAT1) promoter CpG sites and cannabis dependency. In the cases of some DNA sequences, including the DAT1 gene region, their methylation status in blood cells may reflect a systemic modulation in the whole organism. Consequently, we isolated the DNA from the peripheral blood cells from a group of 201 cannabis-dependent patients and 285 controls who were healthy volunteers and who were matched for age and sex. The DNA was subjected to bisulfite conversion and sequencing. Our analysis revealed no statistical differences in the general methylation status of the DAT1 gene promoter CpG island between the patients and controls. Yet, the analysis of individual CpG sites where methylation occurred indicated significant differences. These sites are known to be bound by transcription factors (e.g., SP1, p53, PAX5, or GR), which, apart from other functions, were shown to play a role in the development of the nervous system. Therefore, DAT1 gene promoter methylation studies may provide important insight into the mechanism of cannabis dependency. MDPI 2020-06-23 /pmc/articles/PMC7348832/ /pubmed/32586035 http://dx.doi.org/10.3390/brainsci10060400 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grzywacz, Anna
Barczak, Wojciech
Chmielowiec, Jolanta
Chmielowiec, Krzysztof
Suchanecka, Aleksandra
Trybek, Grzegorz
Masiak, Jolanta
Jagielski, Paweł
Grocholewicz, Katarzyna
Rubiś, Blazej
Contribution of Dopamine Transporter Gene Methylation Status to Cannabis Dependency
title Contribution of Dopamine Transporter Gene Methylation Status to Cannabis Dependency
title_full Contribution of Dopamine Transporter Gene Methylation Status to Cannabis Dependency
title_fullStr Contribution of Dopamine Transporter Gene Methylation Status to Cannabis Dependency
title_full_unstemmed Contribution of Dopamine Transporter Gene Methylation Status to Cannabis Dependency
title_short Contribution of Dopamine Transporter Gene Methylation Status to Cannabis Dependency
title_sort contribution of dopamine transporter gene methylation status to cannabis dependency
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348832/
https://www.ncbi.nlm.nih.gov/pubmed/32586035
http://dx.doi.org/10.3390/brainsci10060400
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