Cargando…

Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes

Both long-term undernutrition and overnutrition disturb metabolic balance, which is mediated partially by the action of two adipokines, leptin and resistin (RSTN). In this study, we manipulated the diet of ewes to produce either a thin (lean) or fat (fat) body condition and investigated how RSTN aff...

Descripción completa

Detalles Bibliográficos
Autores principales: Zieba, Dorota Anna, Biernat, Weronika, Szczesna, Malgorzata, Kirsz, Katarzyna, Barć, Justyna, Misztal, Tomasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348850/
https://www.ncbi.nlm.nih.gov/pubmed/32545900
http://dx.doi.org/10.3390/ijms21124238
_version_ 1783556926756356096
author Zieba, Dorota Anna
Biernat, Weronika
Szczesna, Malgorzata
Kirsz, Katarzyna
Barć, Justyna
Misztal, Tomasz
author_facet Zieba, Dorota Anna
Biernat, Weronika
Szczesna, Malgorzata
Kirsz, Katarzyna
Barć, Justyna
Misztal, Tomasz
author_sort Zieba, Dorota Anna
collection PubMed
description Both long-term undernutrition and overnutrition disturb metabolic balance, which is mediated partially by the action of two adipokines, leptin and resistin (RSTN). In this study, we manipulated the diet of ewes to produce either a thin (lean) or fat (fat) body condition and investigated how RSTN affects endocrine and metabolic status under different leptin concentrations. Twenty ewes were distributed into four groups (n = 5): the lean and fat groups were administered with saline (Lean and Fat), while the Lean-R (Lean-Resistin treated) and Fat-R (Fat-Resistin treated) groups received recombinant bovine resistin. Plasma was assayed for LH, FSH, PRL, RSTN, leptin, GH, glucose, insulin, total cholesterol, nonesterified fatty acid (NEFA), high-density lipoprotein (HDL)-cholesterol, low-density lipoprotein (LDL)-cholesterol and triglycerides. Expression levels of a suppressor of cytokine signaling (SOCS-3) and the long form of the leptin receptor (LRb) were determined in selected brain regions, such as the anterior pituitary, hypothalamic arcuate nucleus, preoptic area and ventro- and dorsomedial nuclei. The results indicate long-term alterations in body weight affect RSTN-mediated effects on metabolic and reproductive hormones concentrations and the expression of leptin signaling components: LRb and SOCS-3. This may be an adaptive mechanism to long-term changes in adiposity during the state of long-day leptin resistance.
format Online
Article
Text
id pubmed-7348850
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73488502020-07-21 Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes Zieba, Dorota Anna Biernat, Weronika Szczesna, Malgorzata Kirsz, Katarzyna Barć, Justyna Misztal, Tomasz Int J Mol Sci Article Both long-term undernutrition and overnutrition disturb metabolic balance, which is mediated partially by the action of two adipokines, leptin and resistin (RSTN). In this study, we manipulated the diet of ewes to produce either a thin (lean) or fat (fat) body condition and investigated how RSTN affects endocrine and metabolic status under different leptin concentrations. Twenty ewes were distributed into four groups (n = 5): the lean and fat groups were administered with saline (Lean and Fat), while the Lean-R (Lean-Resistin treated) and Fat-R (Fat-Resistin treated) groups received recombinant bovine resistin. Plasma was assayed for LH, FSH, PRL, RSTN, leptin, GH, glucose, insulin, total cholesterol, nonesterified fatty acid (NEFA), high-density lipoprotein (HDL)-cholesterol, low-density lipoprotein (LDL)-cholesterol and triglycerides. Expression levels of a suppressor of cytokine signaling (SOCS-3) and the long form of the leptin receptor (LRb) were determined in selected brain regions, such as the anterior pituitary, hypothalamic arcuate nucleus, preoptic area and ventro- and dorsomedial nuclei. The results indicate long-term alterations in body weight affect RSTN-mediated effects on metabolic and reproductive hormones concentrations and the expression of leptin signaling components: LRb and SOCS-3. This may be an adaptive mechanism to long-term changes in adiposity during the state of long-day leptin resistance. MDPI 2020-06-14 /pmc/articles/PMC7348850/ /pubmed/32545900 http://dx.doi.org/10.3390/ijms21124238 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zieba, Dorota Anna
Biernat, Weronika
Szczesna, Malgorzata
Kirsz, Katarzyna
Barć, Justyna
Misztal, Tomasz
Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes
title Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes
title_full Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes
title_fullStr Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes
title_full_unstemmed Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes
title_short Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes
title_sort changes in expression of the genes for the leptin signaling in hypothalamic-pituitary selected areas and endocrine responses to long-term manipulation in body weight and resistin in ewes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348850/
https://www.ncbi.nlm.nih.gov/pubmed/32545900
http://dx.doi.org/10.3390/ijms21124238
work_keys_str_mv AT ziebadorotaanna changesinexpressionofthegenesfortheleptinsignalinginhypothalamicpituitaryselectedareasandendocrineresponsestolongtermmanipulationinbodyweightandresistininewes
AT biernatweronika changesinexpressionofthegenesfortheleptinsignalinginhypothalamicpituitaryselectedareasandendocrineresponsestolongtermmanipulationinbodyweightandresistininewes
AT szczesnamalgorzata changesinexpressionofthegenesfortheleptinsignalinginhypothalamicpituitaryselectedareasandendocrineresponsestolongtermmanipulationinbodyweightandresistininewes
AT kirszkatarzyna changesinexpressionofthegenesfortheleptinsignalinginhypothalamicpituitaryselectedareasandendocrineresponsestolongtermmanipulationinbodyweightandresistininewes
AT barcjustyna changesinexpressionofthegenesfortheleptinsignalinginhypothalamicpituitaryselectedareasandendocrineresponsestolongtermmanipulationinbodyweightandresistininewes
AT misztaltomasz changesinexpressionofthegenesfortheleptinsignalinginhypothalamicpituitaryselectedareasandendocrineresponsestolongtermmanipulationinbodyweightandresistininewes