Cargando…
Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes
Both long-term undernutrition and overnutrition disturb metabolic balance, which is mediated partially by the action of two adipokines, leptin and resistin (RSTN). In this study, we manipulated the diet of ewes to produce either a thin (lean) or fat (fat) body condition and investigated how RSTN aff...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348850/ https://www.ncbi.nlm.nih.gov/pubmed/32545900 http://dx.doi.org/10.3390/ijms21124238 |
_version_ | 1783556926756356096 |
---|---|
author | Zieba, Dorota Anna Biernat, Weronika Szczesna, Malgorzata Kirsz, Katarzyna Barć, Justyna Misztal, Tomasz |
author_facet | Zieba, Dorota Anna Biernat, Weronika Szczesna, Malgorzata Kirsz, Katarzyna Barć, Justyna Misztal, Tomasz |
author_sort | Zieba, Dorota Anna |
collection | PubMed |
description | Both long-term undernutrition and overnutrition disturb metabolic balance, which is mediated partially by the action of two adipokines, leptin and resistin (RSTN). In this study, we manipulated the diet of ewes to produce either a thin (lean) or fat (fat) body condition and investigated how RSTN affects endocrine and metabolic status under different leptin concentrations. Twenty ewes were distributed into four groups (n = 5): the lean and fat groups were administered with saline (Lean and Fat), while the Lean-R (Lean-Resistin treated) and Fat-R (Fat-Resistin treated) groups received recombinant bovine resistin. Plasma was assayed for LH, FSH, PRL, RSTN, leptin, GH, glucose, insulin, total cholesterol, nonesterified fatty acid (NEFA), high-density lipoprotein (HDL)-cholesterol, low-density lipoprotein (LDL)-cholesterol and triglycerides. Expression levels of a suppressor of cytokine signaling (SOCS-3) and the long form of the leptin receptor (LRb) were determined in selected brain regions, such as the anterior pituitary, hypothalamic arcuate nucleus, preoptic area and ventro- and dorsomedial nuclei. The results indicate long-term alterations in body weight affect RSTN-mediated effects on metabolic and reproductive hormones concentrations and the expression of leptin signaling components: LRb and SOCS-3. This may be an adaptive mechanism to long-term changes in adiposity during the state of long-day leptin resistance. |
format | Online Article Text |
id | pubmed-7348850 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73488502020-07-21 Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes Zieba, Dorota Anna Biernat, Weronika Szczesna, Malgorzata Kirsz, Katarzyna Barć, Justyna Misztal, Tomasz Int J Mol Sci Article Both long-term undernutrition and overnutrition disturb metabolic balance, which is mediated partially by the action of two adipokines, leptin and resistin (RSTN). In this study, we manipulated the diet of ewes to produce either a thin (lean) or fat (fat) body condition and investigated how RSTN affects endocrine and metabolic status under different leptin concentrations. Twenty ewes were distributed into four groups (n = 5): the lean and fat groups were administered with saline (Lean and Fat), while the Lean-R (Lean-Resistin treated) and Fat-R (Fat-Resistin treated) groups received recombinant bovine resistin. Plasma was assayed for LH, FSH, PRL, RSTN, leptin, GH, glucose, insulin, total cholesterol, nonesterified fatty acid (NEFA), high-density lipoprotein (HDL)-cholesterol, low-density lipoprotein (LDL)-cholesterol and triglycerides. Expression levels of a suppressor of cytokine signaling (SOCS-3) and the long form of the leptin receptor (LRb) were determined in selected brain regions, such as the anterior pituitary, hypothalamic arcuate nucleus, preoptic area and ventro- and dorsomedial nuclei. The results indicate long-term alterations in body weight affect RSTN-mediated effects on metabolic and reproductive hormones concentrations and the expression of leptin signaling components: LRb and SOCS-3. This may be an adaptive mechanism to long-term changes in adiposity during the state of long-day leptin resistance. MDPI 2020-06-14 /pmc/articles/PMC7348850/ /pubmed/32545900 http://dx.doi.org/10.3390/ijms21124238 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zieba, Dorota Anna Biernat, Weronika Szczesna, Malgorzata Kirsz, Katarzyna Barć, Justyna Misztal, Tomasz Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes |
title | Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes |
title_full | Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes |
title_fullStr | Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes |
title_full_unstemmed | Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes |
title_short | Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes |
title_sort | changes in expression of the genes for the leptin signaling in hypothalamic-pituitary selected areas and endocrine responses to long-term manipulation in body weight and resistin in ewes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348850/ https://www.ncbi.nlm.nih.gov/pubmed/32545900 http://dx.doi.org/10.3390/ijms21124238 |
work_keys_str_mv | AT ziebadorotaanna changesinexpressionofthegenesfortheleptinsignalinginhypothalamicpituitaryselectedareasandendocrineresponsestolongtermmanipulationinbodyweightandresistininewes AT biernatweronika changesinexpressionofthegenesfortheleptinsignalinginhypothalamicpituitaryselectedareasandendocrineresponsestolongtermmanipulationinbodyweightandresistininewes AT szczesnamalgorzata changesinexpressionofthegenesfortheleptinsignalinginhypothalamicpituitaryselectedareasandendocrineresponsestolongtermmanipulationinbodyweightandresistininewes AT kirszkatarzyna changesinexpressionofthegenesfortheleptinsignalinginhypothalamicpituitaryselectedareasandendocrineresponsestolongtermmanipulationinbodyweightandresistininewes AT barcjustyna changesinexpressionofthegenesfortheleptinsignalinginhypothalamicpituitaryselectedareasandendocrineresponsestolongtermmanipulationinbodyweightandresistininewes AT misztaltomasz changesinexpressionofthegenesfortheleptinsignalinginhypothalamicpituitaryselectedareasandendocrineresponsestolongtermmanipulationinbodyweightandresistininewes |