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From Single Cell to Plants: Mesophyll Protoplasts as a Versatile System for Investigating Plant Cell Reprogramming

Plants are sessile organisms that have a remarkable developmental plasticity, which ensures their optimal adaptation to environmental stresses. Plant cell totipotency is an extreme example of such plasticity, whereby somatic cells have the potential to form plants via direct shoot organogenesis or s...

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Detalles Bibliográficos
Autores principales: Pasternak, Taras, Lystvan, Kateryna, Betekhtin, Alexander, Hasterok, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348876/
https://www.ncbi.nlm.nih.gov/pubmed/32545519
http://dx.doi.org/10.3390/ijms21124195
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author Pasternak, Taras
Lystvan, Kateryna
Betekhtin, Alexander
Hasterok, Robert
author_facet Pasternak, Taras
Lystvan, Kateryna
Betekhtin, Alexander
Hasterok, Robert
author_sort Pasternak, Taras
collection PubMed
description Plants are sessile organisms that have a remarkable developmental plasticity, which ensures their optimal adaptation to environmental stresses. Plant cell totipotency is an extreme example of such plasticity, whereby somatic cells have the potential to form plants via direct shoot organogenesis or somatic embryogenesis in response to various exogenous and/or endogenous signals. Protoplasts provide one of the most suitable systems for investigating molecular mechanisms of totipotency, because they are effectively single cell populations. In this review, we consider the current state of knowledge of the mechanisms that induce cell proliferation from individual, differentiated somatic plant cells. We highlight initial explant metabolic status, ploidy level and isolation procedure as determinants of successful cell reprogramming. We also discuss the importance of auxin signalling and its interaction with stress-regulated pathways in governing cell cycle induction and further stages of plant cell totipotency.
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spelling pubmed-73488762020-07-21 From Single Cell to Plants: Mesophyll Protoplasts as a Versatile System for Investigating Plant Cell Reprogramming Pasternak, Taras Lystvan, Kateryna Betekhtin, Alexander Hasterok, Robert Int J Mol Sci Review Plants are sessile organisms that have a remarkable developmental plasticity, which ensures their optimal adaptation to environmental stresses. Plant cell totipotency is an extreme example of such plasticity, whereby somatic cells have the potential to form plants via direct shoot organogenesis or somatic embryogenesis in response to various exogenous and/or endogenous signals. Protoplasts provide one of the most suitable systems for investigating molecular mechanisms of totipotency, because they are effectively single cell populations. In this review, we consider the current state of knowledge of the mechanisms that induce cell proliferation from individual, differentiated somatic plant cells. We highlight initial explant metabolic status, ploidy level and isolation procedure as determinants of successful cell reprogramming. We also discuss the importance of auxin signalling and its interaction with stress-regulated pathways in governing cell cycle induction and further stages of plant cell totipotency. MDPI 2020-06-12 /pmc/articles/PMC7348876/ /pubmed/32545519 http://dx.doi.org/10.3390/ijms21124195 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Pasternak, Taras
Lystvan, Kateryna
Betekhtin, Alexander
Hasterok, Robert
From Single Cell to Plants: Mesophyll Protoplasts as a Versatile System for Investigating Plant Cell Reprogramming
title From Single Cell to Plants: Mesophyll Protoplasts as a Versatile System for Investigating Plant Cell Reprogramming
title_full From Single Cell to Plants: Mesophyll Protoplasts as a Versatile System for Investigating Plant Cell Reprogramming
title_fullStr From Single Cell to Plants: Mesophyll Protoplasts as a Versatile System for Investigating Plant Cell Reprogramming
title_full_unstemmed From Single Cell to Plants: Mesophyll Protoplasts as a Versatile System for Investigating Plant Cell Reprogramming
title_short From Single Cell to Plants: Mesophyll Protoplasts as a Versatile System for Investigating Plant Cell Reprogramming
title_sort from single cell to plants: mesophyll protoplasts as a versatile system for investigating plant cell reprogramming
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348876/
https://www.ncbi.nlm.nih.gov/pubmed/32545519
http://dx.doi.org/10.3390/ijms21124195
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