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The Location of the Pseudoautosomal Boundary in Silene latifolia

Y-chromosomes contain a non-recombining region (NRY), and in many organisms it was shown that the NRY expanded over time. How and why the NRY expands remains unclear. Young sex chromosomes, where NRY expansion occurred recently or is on-going, offer an opportunity to study the causes of this process...

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Autores principales: Krasovec, Marc, Zhang, Yu, Filatov, Dmitry A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348893/
https://www.ncbi.nlm.nih.gov/pubmed/32486434
http://dx.doi.org/10.3390/genes11060610
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author Krasovec, Marc
Zhang, Yu
Filatov, Dmitry A.
author_facet Krasovec, Marc
Zhang, Yu
Filatov, Dmitry A.
author_sort Krasovec, Marc
collection PubMed
description Y-chromosomes contain a non-recombining region (NRY), and in many organisms it was shown that the NRY expanded over time. How and why the NRY expands remains unclear. Young sex chromosomes, where NRY expansion occurred recently or is on-going, offer an opportunity to study the causes of this process. Here, we used the plant Silene latifolia, where sex chromosomes evolved ~11 million years ago, to study the location of the boundary between the NRY and the recombining pseudoautosomal region (PAR). The previous work devoted to the NRY/PAR boundary in S. latifolia was based on a handful of genes with locations approximately known from the genetic map. Here, we report the analysis of 86 pseudoautosomal and sex-linked genes adjacent to the S. latifolia NRY/PAR boundary to establish the location of the boundary more precisely. We take advantage of the dense genetic map and polymorphism data from wild populations to identify 20 partially sex-linked genes located in the “fuzzy boundary”, that rarely recombines in male meiosis. Genes proximal to this fuzzy boundary show no evidence of recombination in males, while the genes distal to this partially-sex-linked region are actively recombining in males. Our results provide a more accurate location for the PAR boundary in S. latifolia, which will help to elucidate the causes of PAR boundary shifts leading to NRY expansion over time.
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spelling pubmed-73488932020-07-22 The Location of the Pseudoautosomal Boundary in Silene latifolia Krasovec, Marc Zhang, Yu Filatov, Dmitry A. Genes (Basel) Article Y-chromosomes contain a non-recombining region (NRY), and in many organisms it was shown that the NRY expanded over time. How and why the NRY expands remains unclear. Young sex chromosomes, where NRY expansion occurred recently or is on-going, offer an opportunity to study the causes of this process. Here, we used the plant Silene latifolia, where sex chromosomes evolved ~11 million years ago, to study the location of the boundary between the NRY and the recombining pseudoautosomal region (PAR). The previous work devoted to the NRY/PAR boundary in S. latifolia was based on a handful of genes with locations approximately known from the genetic map. Here, we report the analysis of 86 pseudoautosomal and sex-linked genes adjacent to the S. latifolia NRY/PAR boundary to establish the location of the boundary more precisely. We take advantage of the dense genetic map and polymorphism data from wild populations to identify 20 partially sex-linked genes located in the “fuzzy boundary”, that rarely recombines in male meiosis. Genes proximal to this fuzzy boundary show no evidence of recombination in males, while the genes distal to this partially-sex-linked region are actively recombining in males. Our results provide a more accurate location for the PAR boundary in S. latifolia, which will help to elucidate the causes of PAR boundary shifts leading to NRY expansion over time. MDPI 2020-05-31 /pmc/articles/PMC7348893/ /pubmed/32486434 http://dx.doi.org/10.3390/genes11060610 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Krasovec, Marc
Zhang, Yu
Filatov, Dmitry A.
The Location of the Pseudoautosomal Boundary in Silene latifolia
title The Location of the Pseudoautosomal Boundary in Silene latifolia
title_full The Location of the Pseudoautosomal Boundary in Silene latifolia
title_fullStr The Location of the Pseudoautosomal Boundary in Silene latifolia
title_full_unstemmed The Location of the Pseudoautosomal Boundary in Silene latifolia
title_short The Location of the Pseudoautosomal Boundary in Silene latifolia
title_sort location of the pseudoautosomal boundary in silene latifolia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348893/
https://www.ncbi.nlm.nih.gov/pubmed/32486434
http://dx.doi.org/10.3390/genes11060610
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