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The Anti-Inflammatory and Pain-Relieving Effects of AR170, an Adenosine A(3) Receptor Agonist, in a Rat Model of Colitis
The pharmacological activation of A(3) receptors has shown potential usefulness in the management of bowel inflammation. However, the role of these receptors in the control of visceral hypersensitivity in the presence of intestinal inflammation has not been investigated. The effects of AR170, a pote...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348903/ https://www.ncbi.nlm.nih.gov/pubmed/32575844 http://dx.doi.org/10.3390/cells9061509 |
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author | Antonioli, Luca Lucarini, Elena Lambertucci, Catia Fornai, Matteo Pellegrini, Carolina Benvenuti, Laura Di Cesare Mannelli, Lorenzo Spinaci, Andrea Marucci, Gabriella Blandizzi, Corrado Ghelardini, Carla Volpini, Rosaria Dal Ben, Diego |
author_facet | Antonioli, Luca Lucarini, Elena Lambertucci, Catia Fornai, Matteo Pellegrini, Carolina Benvenuti, Laura Di Cesare Mannelli, Lorenzo Spinaci, Andrea Marucci, Gabriella Blandizzi, Corrado Ghelardini, Carla Volpini, Rosaria Dal Ben, Diego |
author_sort | Antonioli, Luca |
collection | PubMed |
description | The pharmacological activation of A(3) receptors has shown potential usefulness in the management of bowel inflammation. However, the role of these receptors in the control of visceral hypersensitivity in the presence of intestinal inflammation has not been investigated. The effects of AR170, a potent and selective A(3) receptor agonist, and dexamethasone (DEX) were tested in rats with 2,4-dinitrobenzene sulfonic acid (DNBS)-induced colitis to assess their tissue inflammatory parameters. The animals received AR170, DEX, or a vehicle intraperitoneally for 6 days, starting 1 day before the induction of colitis. Visceral pain was assessed by recording the abdominal responses to colorectal distension in animals with colitis. Colitis was associated with a decrease in body weight and an increase in spleen weight. The macroscopic damage score and tissue tumor necrosis factor (TNF), interleukin 1β (IL-1β), and myeloperoxidase (MPO) levels were also enhanced. AR170, but not DEX, improved body weight. Both drugs counteracted the increase in spleen weight, ameliorated macroscopic colonic damage, and decreased TNF, IL-1β, and MPO tissue levels. The enhanced visceromotor response (VMR) in rats with colitis was decreased via AR170 administration. In rats with colitis, AR170 counteracted colonic inflammatory cell infiltration and decreased pro-inflammatory cytokine levels, thereby relieving visceral hypersensitivity. |
format | Online Article Text |
id | pubmed-7348903 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73489032020-07-22 The Anti-Inflammatory and Pain-Relieving Effects of AR170, an Adenosine A(3) Receptor Agonist, in a Rat Model of Colitis Antonioli, Luca Lucarini, Elena Lambertucci, Catia Fornai, Matteo Pellegrini, Carolina Benvenuti, Laura Di Cesare Mannelli, Lorenzo Spinaci, Andrea Marucci, Gabriella Blandizzi, Corrado Ghelardini, Carla Volpini, Rosaria Dal Ben, Diego Cells Article The pharmacological activation of A(3) receptors has shown potential usefulness in the management of bowel inflammation. However, the role of these receptors in the control of visceral hypersensitivity in the presence of intestinal inflammation has not been investigated. The effects of AR170, a potent and selective A(3) receptor agonist, and dexamethasone (DEX) were tested in rats with 2,4-dinitrobenzene sulfonic acid (DNBS)-induced colitis to assess their tissue inflammatory parameters. The animals received AR170, DEX, or a vehicle intraperitoneally for 6 days, starting 1 day before the induction of colitis. Visceral pain was assessed by recording the abdominal responses to colorectal distension in animals with colitis. Colitis was associated with a decrease in body weight and an increase in spleen weight. The macroscopic damage score and tissue tumor necrosis factor (TNF), interleukin 1β (IL-1β), and myeloperoxidase (MPO) levels were also enhanced. AR170, but not DEX, improved body weight. Both drugs counteracted the increase in spleen weight, ameliorated macroscopic colonic damage, and decreased TNF, IL-1β, and MPO tissue levels. The enhanced visceromotor response (VMR) in rats with colitis was decreased via AR170 administration. In rats with colitis, AR170 counteracted colonic inflammatory cell infiltration and decreased pro-inflammatory cytokine levels, thereby relieving visceral hypersensitivity. MDPI 2020-06-21 /pmc/articles/PMC7348903/ /pubmed/32575844 http://dx.doi.org/10.3390/cells9061509 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Antonioli, Luca Lucarini, Elena Lambertucci, Catia Fornai, Matteo Pellegrini, Carolina Benvenuti, Laura Di Cesare Mannelli, Lorenzo Spinaci, Andrea Marucci, Gabriella Blandizzi, Corrado Ghelardini, Carla Volpini, Rosaria Dal Ben, Diego The Anti-Inflammatory and Pain-Relieving Effects of AR170, an Adenosine A(3) Receptor Agonist, in a Rat Model of Colitis |
title | The Anti-Inflammatory and Pain-Relieving Effects of AR170, an Adenosine A(3) Receptor Agonist, in a Rat Model of Colitis |
title_full | The Anti-Inflammatory and Pain-Relieving Effects of AR170, an Adenosine A(3) Receptor Agonist, in a Rat Model of Colitis |
title_fullStr | The Anti-Inflammatory and Pain-Relieving Effects of AR170, an Adenosine A(3) Receptor Agonist, in a Rat Model of Colitis |
title_full_unstemmed | The Anti-Inflammatory and Pain-Relieving Effects of AR170, an Adenosine A(3) Receptor Agonist, in a Rat Model of Colitis |
title_short | The Anti-Inflammatory and Pain-Relieving Effects of AR170, an Adenosine A(3) Receptor Agonist, in a Rat Model of Colitis |
title_sort | anti-inflammatory and pain-relieving effects of ar170, an adenosine a(3) receptor agonist, in a rat model of colitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348903/ https://www.ncbi.nlm.nih.gov/pubmed/32575844 http://dx.doi.org/10.3390/cells9061509 |
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