Cargando…

Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay

The use of lateral flow immunoassays (LFIAs) for rapid on-site testing is restricted by their relatively high limit of detection (LoD). One possible way to decrease the LoD is to optimize nanoparticle properties that are used as labels. We compare two types of Au nanoparticles: usual quasispherical...

Descripción completa

Detalles Bibliográficos
Autores principales: Byzova, Nadezhda A., Zherdev, Anatoly V., Khlebtsov, Boris N., Burov, Andrey M., Khlebtsov, Nikolai G., Dzantiev, Boris B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348961/
https://www.ncbi.nlm.nih.gov/pubmed/32604874
http://dx.doi.org/10.3390/s20123608
_version_ 1783556953130139648
author Byzova, Nadezhda A.
Zherdev, Anatoly V.
Khlebtsov, Boris N.
Burov, Andrey M.
Khlebtsov, Nikolai G.
Dzantiev, Boris B.
author_facet Byzova, Nadezhda A.
Zherdev, Anatoly V.
Khlebtsov, Boris N.
Burov, Andrey M.
Khlebtsov, Nikolai G.
Dzantiev, Boris B.
author_sort Byzova, Nadezhda A.
collection PubMed
description The use of lateral flow immunoassays (LFIAs) for rapid on-site testing is restricted by their relatively high limit of detection (LoD). One possible way to decrease the LoD is to optimize nanoparticle properties that are used as labels. We compare two types of Au nanoparticles: usual quasispherical gold nanoparticles (C-GNPs), obtained by the Turkevich–Frens method, and superspherical gold nanoparticles (S-GNPs), obtained by a progressive overgrowth technique. Average diameters were 18.6–47.5 nm for C-GNPs and 20.2–90.4 nm for S-GNPs. Cardiomarker troponin I was considered as the target analyte. Adsorption and covalent conjugation with antibodies were tested for both GNP types. For C-GNPs, the minimal LoD was obtained with 33.7 nm nanoparticles, reaching 12.7 ng/mL for covalent immobilization and 9.9 ng/mL for adsorption. The average diameter of S-GNPs varied from 20.2 to 64.5 nm, which resulted in a decrease in LoD for an LFIA of troponin I from 3.4 to 1.2 ng/mL for covalent immobilization and from 2.9 to 2.0 ng/mL for adsorption. Thus, we obtained an 8-fold decrease in LoD (9.9 to 1.2 ng/mL) by using S-GNPs. This effect can be related to more effective antibody immobilization and improved S-GNP optical properties. The obtained results can improve LFIAs for various practically significant analytes.
format Online
Article
Text
id pubmed-7348961
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73489612020-07-22 Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay Byzova, Nadezhda A. Zherdev, Anatoly V. Khlebtsov, Boris N. Burov, Andrey M. Khlebtsov, Nikolai G. Dzantiev, Boris B. Sensors (Basel) Article The use of lateral flow immunoassays (LFIAs) for rapid on-site testing is restricted by their relatively high limit of detection (LoD). One possible way to decrease the LoD is to optimize nanoparticle properties that are used as labels. We compare two types of Au nanoparticles: usual quasispherical gold nanoparticles (C-GNPs), obtained by the Turkevich–Frens method, and superspherical gold nanoparticles (S-GNPs), obtained by a progressive overgrowth technique. Average diameters were 18.6–47.5 nm for C-GNPs and 20.2–90.4 nm for S-GNPs. Cardiomarker troponin I was considered as the target analyte. Adsorption and covalent conjugation with antibodies were tested for both GNP types. For C-GNPs, the minimal LoD was obtained with 33.7 nm nanoparticles, reaching 12.7 ng/mL for covalent immobilization and 9.9 ng/mL for adsorption. The average diameter of S-GNPs varied from 20.2 to 64.5 nm, which resulted in a decrease in LoD for an LFIA of troponin I from 3.4 to 1.2 ng/mL for covalent immobilization and from 2.9 to 2.0 ng/mL for adsorption. Thus, we obtained an 8-fold decrease in LoD (9.9 to 1.2 ng/mL) by using S-GNPs. This effect can be related to more effective antibody immobilization and improved S-GNP optical properties. The obtained results can improve LFIAs for various practically significant analytes. MDPI 2020-06-26 /pmc/articles/PMC7348961/ /pubmed/32604874 http://dx.doi.org/10.3390/s20123608 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Byzova, Nadezhda A.
Zherdev, Anatoly V.
Khlebtsov, Boris N.
Burov, Andrey M.
Khlebtsov, Nikolai G.
Dzantiev, Boris B.
Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay
title Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay
title_full Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay
title_fullStr Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay
title_full_unstemmed Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay
title_short Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay
title_sort advantages of highly spherical gold nanoparticles as labels for lateral flow immunoassay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348961/
https://www.ncbi.nlm.nih.gov/pubmed/32604874
http://dx.doi.org/10.3390/s20123608
work_keys_str_mv AT byzovanadezhdaa advantagesofhighlysphericalgoldnanoparticlesaslabelsforlateralflowimmunoassay
AT zherdevanatolyv advantagesofhighlysphericalgoldnanoparticlesaslabelsforlateralflowimmunoassay
AT khlebtsovborisn advantagesofhighlysphericalgoldnanoparticlesaslabelsforlateralflowimmunoassay
AT burovandreym advantagesofhighlysphericalgoldnanoparticlesaslabelsforlateralflowimmunoassay
AT khlebtsovnikolaig advantagesofhighlysphericalgoldnanoparticlesaslabelsforlateralflowimmunoassay
AT dzantievborisb advantagesofhighlysphericalgoldnanoparticlesaslabelsforlateralflowimmunoassay