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Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay
The use of lateral flow immunoassays (LFIAs) for rapid on-site testing is restricted by their relatively high limit of detection (LoD). One possible way to decrease the LoD is to optimize nanoparticle properties that are used as labels. We compare two types of Au nanoparticles: usual quasispherical...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348961/ https://www.ncbi.nlm.nih.gov/pubmed/32604874 http://dx.doi.org/10.3390/s20123608 |
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author | Byzova, Nadezhda A. Zherdev, Anatoly V. Khlebtsov, Boris N. Burov, Andrey M. Khlebtsov, Nikolai G. Dzantiev, Boris B. |
author_facet | Byzova, Nadezhda A. Zherdev, Anatoly V. Khlebtsov, Boris N. Burov, Andrey M. Khlebtsov, Nikolai G. Dzantiev, Boris B. |
author_sort | Byzova, Nadezhda A. |
collection | PubMed |
description | The use of lateral flow immunoassays (LFIAs) for rapid on-site testing is restricted by their relatively high limit of detection (LoD). One possible way to decrease the LoD is to optimize nanoparticle properties that are used as labels. We compare two types of Au nanoparticles: usual quasispherical gold nanoparticles (C-GNPs), obtained by the Turkevich–Frens method, and superspherical gold nanoparticles (S-GNPs), obtained by a progressive overgrowth technique. Average diameters were 18.6–47.5 nm for C-GNPs and 20.2–90.4 nm for S-GNPs. Cardiomarker troponin I was considered as the target analyte. Adsorption and covalent conjugation with antibodies were tested for both GNP types. For C-GNPs, the minimal LoD was obtained with 33.7 nm nanoparticles, reaching 12.7 ng/mL for covalent immobilization and 9.9 ng/mL for adsorption. The average diameter of S-GNPs varied from 20.2 to 64.5 nm, which resulted in a decrease in LoD for an LFIA of troponin I from 3.4 to 1.2 ng/mL for covalent immobilization and from 2.9 to 2.0 ng/mL for adsorption. Thus, we obtained an 8-fold decrease in LoD (9.9 to 1.2 ng/mL) by using S-GNPs. This effect can be related to more effective antibody immobilization and improved S-GNP optical properties. The obtained results can improve LFIAs for various practically significant analytes. |
format | Online Article Text |
id | pubmed-7348961 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73489612020-07-22 Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay Byzova, Nadezhda A. Zherdev, Anatoly V. Khlebtsov, Boris N. Burov, Andrey M. Khlebtsov, Nikolai G. Dzantiev, Boris B. Sensors (Basel) Article The use of lateral flow immunoassays (LFIAs) for rapid on-site testing is restricted by their relatively high limit of detection (LoD). One possible way to decrease the LoD is to optimize nanoparticle properties that are used as labels. We compare two types of Au nanoparticles: usual quasispherical gold nanoparticles (C-GNPs), obtained by the Turkevich–Frens method, and superspherical gold nanoparticles (S-GNPs), obtained by a progressive overgrowth technique. Average diameters were 18.6–47.5 nm for C-GNPs and 20.2–90.4 nm for S-GNPs. Cardiomarker troponin I was considered as the target analyte. Adsorption and covalent conjugation with antibodies were tested for both GNP types. For C-GNPs, the minimal LoD was obtained with 33.7 nm nanoparticles, reaching 12.7 ng/mL for covalent immobilization and 9.9 ng/mL for adsorption. The average diameter of S-GNPs varied from 20.2 to 64.5 nm, which resulted in a decrease in LoD for an LFIA of troponin I from 3.4 to 1.2 ng/mL for covalent immobilization and from 2.9 to 2.0 ng/mL for adsorption. Thus, we obtained an 8-fold decrease in LoD (9.9 to 1.2 ng/mL) by using S-GNPs. This effect can be related to more effective antibody immobilization and improved S-GNP optical properties. The obtained results can improve LFIAs for various practically significant analytes. MDPI 2020-06-26 /pmc/articles/PMC7348961/ /pubmed/32604874 http://dx.doi.org/10.3390/s20123608 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Byzova, Nadezhda A. Zherdev, Anatoly V. Khlebtsov, Boris N. Burov, Andrey M. Khlebtsov, Nikolai G. Dzantiev, Boris B. Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay |
title | Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay |
title_full | Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay |
title_fullStr | Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay |
title_full_unstemmed | Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay |
title_short | Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay |
title_sort | advantages of highly spherical gold nanoparticles as labels for lateral flow immunoassay |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348961/ https://www.ncbi.nlm.nih.gov/pubmed/32604874 http://dx.doi.org/10.3390/s20123608 |
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