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A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex

The mammalian centrosome/cilium complex is composed of the centrosome, the primary cilium and the centriolar satellites, which together regulate cell polarity, signaling, proliferation and motility in cells and thereby development and homeostasis in organisms. Accordingly, deregulation of its struct...

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Autores principales: Arslanhan, Melis Dilara, Gulensoy, Dila, Firat-Karalar, Elif Nur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348975/
https://www.ncbi.nlm.nih.gov/pubmed/32503249
http://dx.doi.org/10.3390/cells9061390
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author Arslanhan, Melis Dilara
Gulensoy, Dila
Firat-Karalar, Elif Nur
author_facet Arslanhan, Melis Dilara
Gulensoy, Dila
Firat-Karalar, Elif Nur
author_sort Arslanhan, Melis Dilara
collection PubMed
description The mammalian centrosome/cilium complex is composed of the centrosome, the primary cilium and the centriolar satellites, which together regulate cell polarity, signaling, proliferation and motility in cells and thereby development and homeostasis in organisms. Accordingly, deregulation of its structure and functions is implicated in various human diseases including cancer, developmental disorders and neurodegenerative diseases. To better understand these disease connections, the molecular underpinnings of the assembly, maintenance and dynamic adaptations of the centrosome/cilium complex need to be uncovered with exquisite detail. Application of proximity-based labeling methods to the centrosome/cilium complex generated spatial and temporal interaction maps for its components and provided key insights into these questions. In this review, we first describe the structure and cell cycle-linked regulation of the centrosome/cilium complex. Next, we explain the inherent biochemical and temporal limitations in probing the structure and function of the centrosome/cilium complex and describe how proximity-based labeling approaches have addressed them. Finally, we explore current insights into the knowledge we gained from the proximity mapping studies as it pertains to centrosome and cilium biogenesis and systematic characterization of the centrosome, cilium and centriolar satellite interactomes.
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spelling pubmed-73489752020-07-22 A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex Arslanhan, Melis Dilara Gulensoy, Dila Firat-Karalar, Elif Nur Cells Review The mammalian centrosome/cilium complex is composed of the centrosome, the primary cilium and the centriolar satellites, which together regulate cell polarity, signaling, proliferation and motility in cells and thereby development and homeostasis in organisms. Accordingly, deregulation of its structure and functions is implicated in various human diseases including cancer, developmental disorders and neurodegenerative diseases. To better understand these disease connections, the molecular underpinnings of the assembly, maintenance and dynamic adaptations of the centrosome/cilium complex need to be uncovered with exquisite detail. Application of proximity-based labeling methods to the centrosome/cilium complex generated spatial and temporal interaction maps for its components and provided key insights into these questions. In this review, we first describe the structure and cell cycle-linked regulation of the centrosome/cilium complex. Next, we explain the inherent biochemical and temporal limitations in probing the structure and function of the centrosome/cilium complex and describe how proximity-based labeling approaches have addressed them. Finally, we explore current insights into the knowledge we gained from the proximity mapping studies as it pertains to centrosome and cilium biogenesis and systematic characterization of the centrosome, cilium and centriolar satellite interactomes. MDPI 2020-06-03 /pmc/articles/PMC7348975/ /pubmed/32503249 http://dx.doi.org/10.3390/cells9061390 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Arslanhan, Melis Dilara
Gulensoy, Dila
Firat-Karalar, Elif Nur
A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex
title A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex
title_full A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex
title_fullStr A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex
title_full_unstemmed A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex
title_short A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex
title_sort proximity mapping journey into the biology of the mammalian centrosome/cilium complex
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348975/
https://www.ncbi.nlm.nih.gov/pubmed/32503249
http://dx.doi.org/10.3390/cells9061390
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