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A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex
The mammalian centrosome/cilium complex is composed of the centrosome, the primary cilium and the centriolar satellites, which together regulate cell polarity, signaling, proliferation and motility in cells and thereby development and homeostasis in organisms. Accordingly, deregulation of its struct...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348975/ https://www.ncbi.nlm.nih.gov/pubmed/32503249 http://dx.doi.org/10.3390/cells9061390 |
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author | Arslanhan, Melis Dilara Gulensoy, Dila Firat-Karalar, Elif Nur |
author_facet | Arslanhan, Melis Dilara Gulensoy, Dila Firat-Karalar, Elif Nur |
author_sort | Arslanhan, Melis Dilara |
collection | PubMed |
description | The mammalian centrosome/cilium complex is composed of the centrosome, the primary cilium and the centriolar satellites, which together regulate cell polarity, signaling, proliferation and motility in cells and thereby development and homeostasis in organisms. Accordingly, deregulation of its structure and functions is implicated in various human diseases including cancer, developmental disorders and neurodegenerative diseases. To better understand these disease connections, the molecular underpinnings of the assembly, maintenance and dynamic adaptations of the centrosome/cilium complex need to be uncovered with exquisite detail. Application of proximity-based labeling methods to the centrosome/cilium complex generated spatial and temporal interaction maps for its components and provided key insights into these questions. In this review, we first describe the structure and cell cycle-linked regulation of the centrosome/cilium complex. Next, we explain the inherent biochemical and temporal limitations in probing the structure and function of the centrosome/cilium complex and describe how proximity-based labeling approaches have addressed them. Finally, we explore current insights into the knowledge we gained from the proximity mapping studies as it pertains to centrosome and cilium biogenesis and systematic characterization of the centrosome, cilium and centriolar satellite interactomes. |
format | Online Article Text |
id | pubmed-7348975 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73489752020-07-22 A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex Arslanhan, Melis Dilara Gulensoy, Dila Firat-Karalar, Elif Nur Cells Review The mammalian centrosome/cilium complex is composed of the centrosome, the primary cilium and the centriolar satellites, which together regulate cell polarity, signaling, proliferation and motility in cells and thereby development and homeostasis in organisms. Accordingly, deregulation of its structure and functions is implicated in various human diseases including cancer, developmental disorders and neurodegenerative diseases. To better understand these disease connections, the molecular underpinnings of the assembly, maintenance and dynamic adaptations of the centrosome/cilium complex need to be uncovered with exquisite detail. Application of proximity-based labeling methods to the centrosome/cilium complex generated spatial and temporal interaction maps for its components and provided key insights into these questions. In this review, we first describe the structure and cell cycle-linked regulation of the centrosome/cilium complex. Next, we explain the inherent biochemical and temporal limitations in probing the structure and function of the centrosome/cilium complex and describe how proximity-based labeling approaches have addressed them. Finally, we explore current insights into the knowledge we gained from the proximity mapping studies as it pertains to centrosome and cilium biogenesis and systematic characterization of the centrosome, cilium and centriolar satellite interactomes. MDPI 2020-06-03 /pmc/articles/PMC7348975/ /pubmed/32503249 http://dx.doi.org/10.3390/cells9061390 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Arslanhan, Melis Dilara Gulensoy, Dila Firat-Karalar, Elif Nur A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex |
title | A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex |
title_full | A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex |
title_fullStr | A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex |
title_full_unstemmed | A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex |
title_short | A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex |
title_sort | proximity mapping journey into the biology of the mammalian centrosome/cilium complex |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348975/ https://www.ncbi.nlm.nih.gov/pubmed/32503249 http://dx.doi.org/10.3390/cells9061390 |
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