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Parallel Acquisition of Plasma Membrane Ultrastructure and Cytosolic Protein Localisation in Cultured Cells via Correlated Immunogold SEM
Scanning electron microscopy (SEM) takes advantage of distinct detectors to visualise secondary and back-scattering electrons. Here, we report an integrated approach that relies on these two detection methods to simultaneously acquire correlated information on plasma membrane topography and curvatur...
Autores principales: | Begemann, Isabell, Keller, Ulrike, Nüsse, Harald, Klingauf, Jürgen, Galic, Milos |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349049/ https://www.ncbi.nlm.nih.gov/pubmed/32466457 http://dx.doi.org/10.3390/cells9061329 |
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