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Identification of the Ovine Keratin-Associated Protein 2-1 Gene and Its Sequence Variation in Four Chinese Sheep Breeds

Keratin-associated proteins are important components of wool fibers. The gene encoding the high-sulfur keratin-associated protein 2-1 has been described in humans, but it has not been described in sheep. A basic local alignment search tool nucleotide search of the Ovine Genome Assembly version 4.0 u...

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Autores principales: Wang, Jianqing, Zhou, Huitong, Hickford, Jon G. H., Luo, Yuzhu, Gong, Hua, Hu, Jiang, Liu, Xiu, Li, Shaobin, Song, Yize, Ke, Na, Qiao, Lirong, Wang, Jiqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349075/
https://www.ncbi.nlm.nih.gov/pubmed/32485962
http://dx.doi.org/10.3390/genes11060604
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author Wang, Jianqing
Zhou, Huitong
Hickford, Jon G. H.
Luo, Yuzhu
Gong, Hua
Hu, Jiang
Liu, Xiu
Li, Shaobin
Song, Yize
Ke, Na
Qiao, Lirong
Wang, Jiqing
author_facet Wang, Jianqing
Zhou, Huitong
Hickford, Jon G. H.
Luo, Yuzhu
Gong, Hua
Hu, Jiang
Liu, Xiu
Li, Shaobin
Song, Yize
Ke, Na
Qiao, Lirong
Wang, Jiqing
author_sort Wang, Jianqing
collection PubMed
description Keratin-associated proteins are important components of wool fibers. The gene encoding the high-sulfur keratin-associated protein 2-1 has been described in humans, but it has not been described in sheep. A basic local alignment search tool nucleotide search of the Ovine Genome Assembly version 4.0 using a human keratin-associated protein 2-1 gene sequence revealed a 399-base pair open reading frame, which was clustered among nine previously identified keratin-associated protein genes on chromosome 11. Polymerase chain reaction–single strand conformation polymorphism analysis revealed four different banding patterns, with these representing four different sequences (A–D) in Chinese sheep breeds. These sequences had the highest similarity to human keratin-associated protein 2-1 gene, suggesting that they represent variants of ovine keratin-associated protein 2-1 gene. Nine single nucleotide variations were detected in the gene, including one non-synonymous nucleotide substitution. Differences in variant frequencies between fine-wool sheep breeds and coarse-wool sheep breeds were detected. The gene was found to be expressed in various tissues, with the highest expression level in skin, and moderate expression levels in heart and lung tissue. These results reveal that the ovine keratin-associated protein 2-1 gene is variable and suggest the gene might affect variation in mean fiber diameter.
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spelling pubmed-73490752020-07-22 Identification of the Ovine Keratin-Associated Protein 2-1 Gene and Its Sequence Variation in Four Chinese Sheep Breeds Wang, Jianqing Zhou, Huitong Hickford, Jon G. H. Luo, Yuzhu Gong, Hua Hu, Jiang Liu, Xiu Li, Shaobin Song, Yize Ke, Na Qiao, Lirong Wang, Jiqing Genes (Basel) Article Keratin-associated proteins are important components of wool fibers. The gene encoding the high-sulfur keratin-associated protein 2-1 has been described in humans, but it has not been described in sheep. A basic local alignment search tool nucleotide search of the Ovine Genome Assembly version 4.0 using a human keratin-associated protein 2-1 gene sequence revealed a 399-base pair open reading frame, which was clustered among nine previously identified keratin-associated protein genes on chromosome 11. Polymerase chain reaction–single strand conformation polymorphism analysis revealed four different banding patterns, with these representing four different sequences (A–D) in Chinese sheep breeds. These sequences had the highest similarity to human keratin-associated protein 2-1 gene, suggesting that they represent variants of ovine keratin-associated protein 2-1 gene. Nine single nucleotide variations were detected in the gene, including one non-synonymous nucleotide substitution. Differences in variant frequencies between fine-wool sheep breeds and coarse-wool sheep breeds were detected. The gene was found to be expressed in various tissues, with the highest expression level in skin, and moderate expression levels in heart and lung tissue. These results reveal that the ovine keratin-associated protein 2-1 gene is variable and suggest the gene might affect variation in mean fiber diameter. MDPI 2020-05-29 /pmc/articles/PMC7349075/ /pubmed/32485962 http://dx.doi.org/10.3390/genes11060604 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Jianqing
Zhou, Huitong
Hickford, Jon G. H.
Luo, Yuzhu
Gong, Hua
Hu, Jiang
Liu, Xiu
Li, Shaobin
Song, Yize
Ke, Na
Qiao, Lirong
Wang, Jiqing
Identification of the Ovine Keratin-Associated Protein 2-1 Gene and Its Sequence Variation in Four Chinese Sheep Breeds
title Identification of the Ovine Keratin-Associated Protein 2-1 Gene and Its Sequence Variation in Four Chinese Sheep Breeds
title_full Identification of the Ovine Keratin-Associated Protein 2-1 Gene and Its Sequence Variation in Four Chinese Sheep Breeds
title_fullStr Identification of the Ovine Keratin-Associated Protein 2-1 Gene and Its Sequence Variation in Four Chinese Sheep Breeds
title_full_unstemmed Identification of the Ovine Keratin-Associated Protein 2-1 Gene and Its Sequence Variation in Four Chinese Sheep Breeds
title_short Identification of the Ovine Keratin-Associated Protein 2-1 Gene and Its Sequence Variation in Four Chinese Sheep Breeds
title_sort identification of the ovine keratin-associated protein 2-1 gene and its sequence variation in four chinese sheep breeds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349075/
https://www.ncbi.nlm.nih.gov/pubmed/32485962
http://dx.doi.org/10.3390/genes11060604
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