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Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells
The recent advances, offered by cell therapy in the regenerative medicine field, offer a revolutionary potential for the development of innovative cures to restore compromised physiological functions or organs. Adult myogenic precursors, such as myoblasts or satellite cells, possess a marked regener...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349204/ https://www.ncbi.nlm.nih.gov/pubmed/32585911 http://dx.doi.org/10.3390/cells9061527 |
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author | Baci, Denisa Chirivì, Maila Pace, Valentina Maiullari, Fabio Milan, Marika Rampin, Andrea Somma, Paolo Presutti, Dario Garavelli, Silvia Bruno, Antonino Cannata, Stefano Lanzuolo, Chiara Gargioli, Cesare Rizzi, Roberto Bearzi, Claudia |
author_facet | Baci, Denisa Chirivì, Maila Pace, Valentina Maiullari, Fabio Milan, Marika Rampin, Andrea Somma, Paolo Presutti, Dario Garavelli, Silvia Bruno, Antonino Cannata, Stefano Lanzuolo, Chiara Gargioli, Cesare Rizzi, Roberto Bearzi, Claudia |
author_sort | Baci, Denisa |
collection | PubMed |
description | The recent advances, offered by cell therapy in the regenerative medicine field, offer a revolutionary potential for the development of innovative cures to restore compromised physiological functions or organs. Adult myogenic precursors, such as myoblasts or satellite cells, possess a marked regenerative capacity, but the exploitation of this potential still encounters significant challenges in clinical application, due to low rate of proliferation in vitro, as well as a reduced self-renewal capacity. In this scenario, induced pluripotent stem cells (iPSCs) can offer not only an inexhaustible source of cells for regenerative therapeutic approaches, but also a valuable alternative for in vitro modeling of patient-specific diseases. In this study we established a reliable protocol to induce the myogenic differentiation of iPSCs, generated from pericytes and fibroblasts, exploiting skeletal muscle-derived extracellular vesicles (EVs), in combination with chemically defined factors. This genetic integration-free approach generates functional skeletal myotubes maintaining the engraftment ability in vivo. Our results demonstrate evidence that EVs can act as biological “shuttles” to deliver specific bioactive molecules for a successful transgene-free differentiation offering new opportunities for disease modeling and regenerative approaches. |
format | Online Article Text |
id | pubmed-7349204 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73492042020-07-22 Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells Baci, Denisa Chirivì, Maila Pace, Valentina Maiullari, Fabio Milan, Marika Rampin, Andrea Somma, Paolo Presutti, Dario Garavelli, Silvia Bruno, Antonino Cannata, Stefano Lanzuolo, Chiara Gargioli, Cesare Rizzi, Roberto Bearzi, Claudia Cells Article The recent advances, offered by cell therapy in the regenerative medicine field, offer a revolutionary potential for the development of innovative cures to restore compromised physiological functions or organs. Adult myogenic precursors, such as myoblasts or satellite cells, possess a marked regenerative capacity, but the exploitation of this potential still encounters significant challenges in clinical application, due to low rate of proliferation in vitro, as well as a reduced self-renewal capacity. In this scenario, induced pluripotent stem cells (iPSCs) can offer not only an inexhaustible source of cells for regenerative therapeutic approaches, but also a valuable alternative for in vitro modeling of patient-specific diseases. In this study we established a reliable protocol to induce the myogenic differentiation of iPSCs, generated from pericytes and fibroblasts, exploiting skeletal muscle-derived extracellular vesicles (EVs), in combination with chemically defined factors. This genetic integration-free approach generates functional skeletal myotubes maintaining the engraftment ability in vivo. Our results demonstrate evidence that EVs can act as biological “shuttles” to deliver specific bioactive molecules for a successful transgene-free differentiation offering new opportunities for disease modeling and regenerative approaches. MDPI 2020-06-23 /pmc/articles/PMC7349204/ /pubmed/32585911 http://dx.doi.org/10.3390/cells9061527 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Baci, Denisa Chirivì, Maila Pace, Valentina Maiullari, Fabio Milan, Marika Rampin, Andrea Somma, Paolo Presutti, Dario Garavelli, Silvia Bruno, Antonino Cannata, Stefano Lanzuolo, Chiara Gargioli, Cesare Rizzi, Roberto Bearzi, Claudia Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells |
title | Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells |
title_full | Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells |
title_fullStr | Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells |
title_full_unstemmed | Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells |
title_short | Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells |
title_sort | extracellular vesicles from skeletal muscle cells efficiently promote myogenesis in induced pluripotent stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349204/ https://www.ncbi.nlm.nih.gov/pubmed/32585911 http://dx.doi.org/10.3390/cells9061527 |
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