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Detection of Wolbachia Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, Mayetiola destructor (Say)

Mayetiola destructor (Hessian fly) is a destructive pest of wheat in several parts of the world. Here, we investigated the presence of reproductive symbionts and the effect of the geographical location on the bacterial community associated to adult Hessian flies derived from four major wheat produci...

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Autores principales: Bel Mokhtar, Naima, Maurady, Amal, Britel, Mohammed Reda, El Bouhssini, Mustapha, Batargias, Costas, Stathopoulou, Panagiota, Asimakis, Elias, Tsiamis, George
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349215/
https://www.ncbi.nlm.nih.gov/pubmed/32498270
http://dx.doi.org/10.3390/insects11060340
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author Bel Mokhtar, Naima
Maurady, Amal
Britel, Mohammed Reda
El Bouhssini, Mustapha
Batargias, Costas
Stathopoulou, Panagiota
Asimakis, Elias
Tsiamis, George
author_facet Bel Mokhtar, Naima
Maurady, Amal
Britel, Mohammed Reda
El Bouhssini, Mustapha
Batargias, Costas
Stathopoulou, Panagiota
Asimakis, Elias
Tsiamis, George
author_sort Bel Mokhtar, Naima
collection PubMed
description Mayetiola destructor (Hessian fly) is a destructive pest of wheat in several parts of the world. Here, we investigated the presence of reproductive symbionts and the effect of the geographical location on the bacterial community associated to adult Hessian flies derived from four major wheat producing areas in Morocco. Using specific 16S rDNA PCR assay, Wolbachia infection was observed in 3% of the natural populations and 10% of the laboratory population. High throughput sequencing of V3-V4 region of the bacterial 16S rRNA gene revealed that the microbiota of adult Hessian flies was significantly influenced by their native regions. A total of 6 phyla, 10 classes and 79 genera were obtained from all the samples. Confirming the screening results, Wolbachia was identified as well in the natural Hessian flies. Phylogenetic analysis using the sequences obtained in this study indicated that there is one Wolbachia strain belonging to supergroup A. To our knowledge, this is the first report of Wolbachia in Hessian fly populations. The observed low abundance of Wolbachia most likely does not indicate induction of reproductive incompatibility. Yet, this infection may give a new insight into the use of Wolbachia for the fight against Hessian fly populations.
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spelling pubmed-73492152020-07-22 Detection of Wolbachia Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, Mayetiola destructor (Say) Bel Mokhtar, Naima Maurady, Amal Britel, Mohammed Reda El Bouhssini, Mustapha Batargias, Costas Stathopoulou, Panagiota Asimakis, Elias Tsiamis, George Insects Article Mayetiola destructor (Hessian fly) is a destructive pest of wheat in several parts of the world. Here, we investigated the presence of reproductive symbionts and the effect of the geographical location on the bacterial community associated to adult Hessian flies derived from four major wheat producing areas in Morocco. Using specific 16S rDNA PCR assay, Wolbachia infection was observed in 3% of the natural populations and 10% of the laboratory population. High throughput sequencing of V3-V4 region of the bacterial 16S rRNA gene revealed that the microbiota of adult Hessian flies was significantly influenced by their native regions. A total of 6 phyla, 10 classes and 79 genera were obtained from all the samples. Confirming the screening results, Wolbachia was identified as well in the natural Hessian flies. Phylogenetic analysis using the sequences obtained in this study indicated that there is one Wolbachia strain belonging to supergroup A. To our knowledge, this is the first report of Wolbachia in Hessian fly populations. The observed low abundance of Wolbachia most likely does not indicate induction of reproductive incompatibility. Yet, this infection may give a new insight into the use of Wolbachia for the fight against Hessian fly populations. MDPI 2020-06-02 /pmc/articles/PMC7349215/ /pubmed/32498270 http://dx.doi.org/10.3390/insects11060340 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bel Mokhtar, Naima
Maurady, Amal
Britel, Mohammed Reda
El Bouhssini, Mustapha
Batargias, Costas
Stathopoulou, Panagiota
Asimakis, Elias
Tsiamis, George
Detection of Wolbachia Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, Mayetiola destructor (Say)
title Detection of Wolbachia Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, Mayetiola destructor (Say)
title_full Detection of Wolbachia Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, Mayetiola destructor (Say)
title_fullStr Detection of Wolbachia Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, Mayetiola destructor (Say)
title_full_unstemmed Detection of Wolbachia Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, Mayetiola destructor (Say)
title_short Detection of Wolbachia Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, Mayetiola destructor (Say)
title_sort detection of wolbachia infections in natural and laboratory populations of the moroccan hessian fly, mayetiola destructor (say)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349215/
https://www.ncbi.nlm.nih.gov/pubmed/32498270
http://dx.doi.org/10.3390/insects11060340
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