Cargando…

Membrane Elastic Properties during Neural Precursor Cell Differentiation

Neural precursor cells differentiate into several cell types that display distinct functions. However, little is known about how cell surface mechanics vary during the differentiation process. Here, by precisely measuring membrane tension and bending modulus, we map their variations and correlate th...

Descripción completa

Detalles Bibliográficos
Autores principales: Soares, Juliana, Araujo, Glauber R. de S., Santana, Cintia, Matias, Diana, Moura-Neto, Vivaldo, Farina, Marcos, Frases, Susana, Viana, Nathan B., Romão, Luciana, Nussenzveig, H. Moysés, Pontes, Bruno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349228/
https://www.ncbi.nlm.nih.gov/pubmed/32466390
http://dx.doi.org/10.3390/cells9061323
_version_ 1783557014136291328
author Soares, Juliana
Araujo, Glauber R. de S.
Santana, Cintia
Matias, Diana
Moura-Neto, Vivaldo
Farina, Marcos
Frases, Susana
Viana, Nathan B.
Romão, Luciana
Nussenzveig, H. Moysés
Pontes, Bruno
author_facet Soares, Juliana
Araujo, Glauber R. de S.
Santana, Cintia
Matias, Diana
Moura-Neto, Vivaldo
Farina, Marcos
Frases, Susana
Viana, Nathan B.
Romão, Luciana
Nussenzveig, H. Moysés
Pontes, Bruno
author_sort Soares, Juliana
collection PubMed
description Neural precursor cells differentiate into several cell types that display distinct functions. However, little is known about how cell surface mechanics vary during the differentiation process. Here, by precisely measuring membrane tension and bending modulus, we map their variations and correlate them with changes in neural precursor cell morphology along their distinct differentiation fates. Both cells maintained in culture as neural precursors as well as those plated in neurobasal medium reveal a decrease in membrane tension over the first hours of culture followed by stabilization, with no change in bending modulus. During astrocyte differentiation, membrane tension initially decreases and then increases after 72 h, accompanied by consolidation of glial fibrillary acidic protein expression and striking actin reorganization, while bending modulus increases following observed alterations. For oligodendrocytes, the changes in membrane tension are less abrupt over the first hours, but their values subsequently decrease, correlating with a shift from oligodendrocyte marker O4 to myelin basic protein expressions and a remarkable actin reorganization, while bending modulus remains constant. Oligodendrocytes at later differentiation stages show membrane vesicles with similar membrane tension but higher bending modulus as compared to the cell surface. Altogether, our results display an entire spectrum of how membrane elastic properties are varying, thus contributing to a better understanding of neural differentiation from a mechanobiological perspective.
format Online
Article
Text
id pubmed-7349228
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73492282020-07-22 Membrane Elastic Properties during Neural Precursor Cell Differentiation Soares, Juliana Araujo, Glauber R. de S. Santana, Cintia Matias, Diana Moura-Neto, Vivaldo Farina, Marcos Frases, Susana Viana, Nathan B. Romão, Luciana Nussenzveig, H. Moysés Pontes, Bruno Cells Article Neural precursor cells differentiate into several cell types that display distinct functions. However, little is known about how cell surface mechanics vary during the differentiation process. Here, by precisely measuring membrane tension and bending modulus, we map their variations and correlate them with changes in neural precursor cell morphology along their distinct differentiation fates. Both cells maintained in culture as neural precursors as well as those plated in neurobasal medium reveal a decrease in membrane tension over the first hours of culture followed by stabilization, with no change in bending modulus. During astrocyte differentiation, membrane tension initially decreases and then increases after 72 h, accompanied by consolidation of glial fibrillary acidic protein expression and striking actin reorganization, while bending modulus increases following observed alterations. For oligodendrocytes, the changes in membrane tension are less abrupt over the first hours, but their values subsequently decrease, correlating with a shift from oligodendrocyte marker O4 to myelin basic protein expressions and a remarkable actin reorganization, while bending modulus remains constant. Oligodendrocytes at later differentiation stages show membrane vesicles with similar membrane tension but higher bending modulus as compared to the cell surface. Altogether, our results display an entire spectrum of how membrane elastic properties are varying, thus contributing to a better understanding of neural differentiation from a mechanobiological perspective. MDPI 2020-05-26 /pmc/articles/PMC7349228/ /pubmed/32466390 http://dx.doi.org/10.3390/cells9061323 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Soares, Juliana
Araujo, Glauber R. de S.
Santana, Cintia
Matias, Diana
Moura-Neto, Vivaldo
Farina, Marcos
Frases, Susana
Viana, Nathan B.
Romão, Luciana
Nussenzveig, H. Moysés
Pontes, Bruno
Membrane Elastic Properties during Neural Precursor Cell Differentiation
title Membrane Elastic Properties during Neural Precursor Cell Differentiation
title_full Membrane Elastic Properties during Neural Precursor Cell Differentiation
title_fullStr Membrane Elastic Properties during Neural Precursor Cell Differentiation
title_full_unstemmed Membrane Elastic Properties during Neural Precursor Cell Differentiation
title_short Membrane Elastic Properties during Neural Precursor Cell Differentiation
title_sort membrane elastic properties during neural precursor cell differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349228/
https://www.ncbi.nlm.nih.gov/pubmed/32466390
http://dx.doi.org/10.3390/cells9061323
work_keys_str_mv AT soaresjuliana membraneelasticpropertiesduringneuralprecursorcelldifferentiation
AT araujoglauberrdes membraneelasticpropertiesduringneuralprecursorcelldifferentiation
AT santanacintia membraneelasticpropertiesduringneuralprecursorcelldifferentiation
AT matiasdiana membraneelasticpropertiesduringneuralprecursorcelldifferentiation
AT mouranetovivaldo membraneelasticpropertiesduringneuralprecursorcelldifferentiation
AT farinamarcos membraneelasticpropertiesduringneuralprecursorcelldifferentiation
AT frasessusana membraneelasticpropertiesduringneuralprecursorcelldifferentiation
AT viananathanb membraneelasticpropertiesduringneuralprecursorcelldifferentiation
AT romaoluciana membraneelasticpropertiesduringneuralprecursorcelldifferentiation
AT nussenzveighmoyses membraneelasticpropertiesduringneuralprecursorcelldifferentiation
AT pontesbruno membraneelasticpropertiesduringneuralprecursorcelldifferentiation