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RNA-Sequencing-Based Transcriptomic Analysis Reveals a Role for Annexin-A1 in Classical and Influenza A Virus-Induced Autophagy

Influenza viruses have been shown to use autophagy for their survival. However, the proteins and mechanisms involved in the autophagic process triggered by the influenza virus are unclear. Annexin-A1 (ANXA1) is an immunomodulatory protein involved in the regulation of the immune response and Influen...

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Autores principales: Cui, Jianzhou, Morgan, Dhakshayini, Cheng, Dao Han, Foo, Sok Lin, Yap, Gracemary L. R., Ampomah, Patrick B., Arora, Suruchi, Sachaphibulkij, Karishma, Periaswamy, Balamurugan, Fairhurst, Anna-Marie, De Sessions, Paola Florez, Lim, Lina H. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349256/
https://www.ncbi.nlm.nih.gov/pubmed/32512864
http://dx.doi.org/10.3390/cells9061399
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author Cui, Jianzhou
Morgan, Dhakshayini
Cheng, Dao Han
Foo, Sok Lin
Yap, Gracemary L. R.
Ampomah, Patrick B.
Arora, Suruchi
Sachaphibulkij, Karishma
Periaswamy, Balamurugan
Fairhurst, Anna-Marie
De Sessions, Paola Florez
Lim, Lina H. K.
author_facet Cui, Jianzhou
Morgan, Dhakshayini
Cheng, Dao Han
Foo, Sok Lin
Yap, Gracemary L. R.
Ampomah, Patrick B.
Arora, Suruchi
Sachaphibulkij, Karishma
Periaswamy, Balamurugan
Fairhurst, Anna-Marie
De Sessions, Paola Florez
Lim, Lina H. K.
author_sort Cui, Jianzhou
collection PubMed
description Influenza viruses have been shown to use autophagy for their survival. However, the proteins and mechanisms involved in the autophagic process triggered by the influenza virus are unclear. Annexin-A1 (ANXA1) is an immunomodulatory protein involved in the regulation of the immune response and Influenza A virus (IAV) replication. In this study, using clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 (CRISPR associated protein 9) deletion of ANXA1, combined with the next-generation sequencing, we systematically analyzed the critical role of ANXA1 in IAV infection as well as the detailed processes governing IAV infection, such as macroautophagy. A number of differentially expressed genes were uniquely expressed in influenza A virus-infected A549 parental cells and A549 ∆ANXA1 cells, which were enriched in the immune system and infection-related pathways. Gene ontology and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway revealed the role of ANXA1 in autophagy. To validate this, the effect of mechanistic target of rapamycin (mTOR) inhibitors, starvation and influenza infection on autophagy was determined, and our results demonstrate that ANXA1 enhances autophagy induced by conventional autophagy inducers and influenza virus. These results will help us to understand the underlying mechanisms of IAV infection and provide a potential therapeutic target for restricting influenza viral replication and infection.
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spelling pubmed-73492562020-07-22 RNA-Sequencing-Based Transcriptomic Analysis Reveals a Role for Annexin-A1 in Classical and Influenza A Virus-Induced Autophagy Cui, Jianzhou Morgan, Dhakshayini Cheng, Dao Han Foo, Sok Lin Yap, Gracemary L. R. Ampomah, Patrick B. Arora, Suruchi Sachaphibulkij, Karishma Periaswamy, Balamurugan Fairhurst, Anna-Marie De Sessions, Paola Florez Lim, Lina H. K. Cells Article Influenza viruses have been shown to use autophagy for their survival. However, the proteins and mechanisms involved in the autophagic process triggered by the influenza virus are unclear. Annexin-A1 (ANXA1) is an immunomodulatory protein involved in the regulation of the immune response and Influenza A virus (IAV) replication. In this study, using clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 (CRISPR associated protein 9) deletion of ANXA1, combined with the next-generation sequencing, we systematically analyzed the critical role of ANXA1 in IAV infection as well as the detailed processes governing IAV infection, such as macroautophagy. A number of differentially expressed genes were uniquely expressed in influenza A virus-infected A549 parental cells and A549 ∆ANXA1 cells, which were enriched in the immune system and infection-related pathways. Gene ontology and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway revealed the role of ANXA1 in autophagy. To validate this, the effect of mechanistic target of rapamycin (mTOR) inhibitors, starvation and influenza infection on autophagy was determined, and our results demonstrate that ANXA1 enhances autophagy induced by conventional autophagy inducers and influenza virus. These results will help us to understand the underlying mechanisms of IAV infection and provide a potential therapeutic target for restricting influenza viral replication and infection. MDPI 2020-06-04 /pmc/articles/PMC7349256/ /pubmed/32512864 http://dx.doi.org/10.3390/cells9061399 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cui, Jianzhou
Morgan, Dhakshayini
Cheng, Dao Han
Foo, Sok Lin
Yap, Gracemary L. R.
Ampomah, Patrick B.
Arora, Suruchi
Sachaphibulkij, Karishma
Periaswamy, Balamurugan
Fairhurst, Anna-Marie
De Sessions, Paola Florez
Lim, Lina H. K.
RNA-Sequencing-Based Transcriptomic Analysis Reveals a Role for Annexin-A1 in Classical and Influenza A Virus-Induced Autophagy
title RNA-Sequencing-Based Transcriptomic Analysis Reveals a Role for Annexin-A1 in Classical and Influenza A Virus-Induced Autophagy
title_full RNA-Sequencing-Based Transcriptomic Analysis Reveals a Role for Annexin-A1 in Classical and Influenza A Virus-Induced Autophagy
title_fullStr RNA-Sequencing-Based Transcriptomic Analysis Reveals a Role for Annexin-A1 in Classical and Influenza A Virus-Induced Autophagy
title_full_unstemmed RNA-Sequencing-Based Transcriptomic Analysis Reveals a Role for Annexin-A1 in Classical and Influenza A Virus-Induced Autophagy
title_short RNA-Sequencing-Based Transcriptomic Analysis Reveals a Role for Annexin-A1 in Classical and Influenza A Virus-Induced Autophagy
title_sort rna-sequencing-based transcriptomic analysis reveals a role for annexin-a1 in classical and influenza a virus-induced autophagy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349256/
https://www.ncbi.nlm.nih.gov/pubmed/32512864
http://dx.doi.org/10.3390/cells9061399
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