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Higher Mast Cell Accumulation in Human Adipose Tissues Defines Clinically Favorable Obesity Sub-Phenotypes
The identification of human obesity sub-types may improve the clinical management of patients with obesity and uncover previously unrecognized obesity mechanisms. Here, we hypothesized that adipose tissue (AT) mast cells (MC) estimation could be a mark for human obesity sub-phenotyping beyond curren...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349306/ https://www.ncbi.nlm.nih.gov/pubmed/32575785 http://dx.doi.org/10.3390/cells9061508 |
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author | Goldstein, Nir Kezerle, Yarden Gepner, Yftach Haim, Yulia Pecht, Tal Gazit, Roi Polischuk, Vera Liberty, Idit F. Kirshtein, Boris Shaco-Levy, Ruthy Blüher, Matthias Rudich, Assaf |
author_facet | Goldstein, Nir Kezerle, Yarden Gepner, Yftach Haim, Yulia Pecht, Tal Gazit, Roi Polischuk, Vera Liberty, Idit F. Kirshtein, Boris Shaco-Levy, Ruthy Blüher, Matthias Rudich, Assaf |
author_sort | Goldstein, Nir |
collection | PubMed |
description | The identification of human obesity sub-types may improve the clinical management of patients with obesity and uncover previously unrecognized obesity mechanisms. Here, we hypothesized that adipose tissue (AT) mast cells (MC) estimation could be a mark for human obesity sub-phenotyping beyond current clinical-based stratifications, both cross-sectionally and prospectively. We estimated MC accumulation using immunohistochemistry and gene expression in abdominal visceral AT (VAT) and subcutaneous (SAT) in a human cohort of 65 persons with obesity who underwent elective abdominal (mainly bariatric) surgery, and we validated key results in two clinically similar, independent cohorts (n = 33, n = 56). AT-MC were readily detectable by immunostaining for either c-kit or tryptase and by assessing the gene expression of KIT (KIT Proto-Oncogene, Receptor Tyrosine Kinase), TPSB2 (tryptase beta 2), and CMA1 (chymase 1). Participants were characterized as VAT-MC(low) if the expression of both CMA1 and TPSB2 was below the median. Higher expressers of MC genes (MC(high)) were metabolically healthier (lower fasting glucose and glycated hemoglobin, with higher pancreatic beta cell reserve (HOMA-β), and lower triglycerides and alkaline-phosphatase) than people with low expression (MC(low)). Prospectively, higher MC accumulation in VAT or SAT obtained during surgery predicted greater postoperative weight-loss response to bariatric surgery. Jointly, high AT-MC accumulation may be used to clinically define obesity sub-phenotypes, which are associated with a “healthier” cardiometabolic risk profile and a better weight-loss response to bariatric surgery. |
format | Online Article Text |
id | pubmed-7349306 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73493062020-07-22 Higher Mast Cell Accumulation in Human Adipose Tissues Defines Clinically Favorable Obesity Sub-Phenotypes Goldstein, Nir Kezerle, Yarden Gepner, Yftach Haim, Yulia Pecht, Tal Gazit, Roi Polischuk, Vera Liberty, Idit F. Kirshtein, Boris Shaco-Levy, Ruthy Blüher, Matthias Rudich, Assaf Cells Article The identification of human obesity sub-types may improve the clinical management of patients with obesity and uncover previously unrecognized obesity mechanisms. Here, we hypothesized that adipose tissue (AT) mast cells (MC) estimation could be a mark for human obesity sub-phenotyping beyond current clinical-based stratifications, both cross-sectionally and prospectively. We estimated MC accumulation using immunohistochemistry and gene expression in abdominal visceral AT (VAT) and subcutaneous (SAT) in a human cohort of 65 persons with obesity who underwent elective abdominal (mainly bariatric) surgery, and we validated key results in two clinically similar, independent cohorts (n = 33, n = 56). AT-MC were readily detectable by immunostaining for either c-kit or tryptase and by assessing the gene expression of KIT (KIT Proto-Oncogene, Receptor Tyrosine Kinase), TPSB2 (tryptase beta 2), and CMA1 (chymase 1). Participants were characterized as VAT-MC(low) if the expression of both CMA1 and TPSB2 was below the median. Higher expressers of MC genes (MC(high)) were metabolically healthier (lower fasting glucose and glycated hemoglobin, with higher pancreatic beta cell reserve (HOMA-β), and lower triglycerides and alkaline-phosphatase) than people with low expression (MC(low)). Prospectively, higher MC accumulation in VAT or SAT obtained during surgery predicted greater postoperative weight-loss response to bariatric surgery. Jointly, high AT-MC accumulation may be used to clinically define obesity sub-phenotypes, which are associated with a “healthier” cardiometabolic risk profile and a better weight-loss response to bariatric surgery. MDPI 2020-06-20 /pmc/articles/PMC7349306/ /pubmed/32575785 http://dx.doi.org/10.3390/cells9061508 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Goldstein, Nir Kezerle, Yarden Gepner, Yftach Haim, Yulia Pecht, Tal Gazit, Roi Polischuk, Vera Liberty, Idit F. Kirshtein, Boris Shaco-Levy, Ruthy Blüher, Matthias Rudich, Assaf Higher Mast Cell Accumulation in Human Adipose Tissues Defines Clinically Favorable Obesity Sub-Phenotypes |
title | Higher Mast Cell Accumulation in Human Adipose Tissues Defines Clinically Favorable Obesity Sub-Phenotypes |
title_full | Higher Mast Cell Accumulation in Human Adipose Tissues Defines Clinically Favorable Obesity Sub-Phenotypes |
title_fullStr | Higher Mast Cell Accumulation in Human Adipose Tissues Defines Clinically Favorable Obesity Sub-Phenotypes |
title_full_unstemmed | Higher Mast Cell Accumulation in Human Adipose Tissues Defines Clinically Favorable Obesity Sub-Phenotypes |
title_short | Higher Mast Cell Accumulation in Human Adipose Tissues Defines Clinically Favorable Obesity Sub-Phenotypes |
title_sort | higher mast cell accumulation in human adipose tissues defines clinically favorable obesity sub-phenotypes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349306/ https://www.ncbi.nlm.nih.gov/pubmed/32575785 http://dx.doi.org/10.3390/cells9061508 |
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