Cargando…

Rapid Direct Nucleic Acid Amplification Test without RNA Extraction for SARS-CoV-2 Using a Portable PCR Thermocycler

There is an ongoing worldwide coronavirus disease 2019 (Covid-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). At present, confirmatory diagnosis is by reverse transcription polymerase chain reaction (RT-PCR), typically taking several hours and requiring a molecul...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wee, Soon Keong, Sivalingam, Suppiah Paramalingam, Yap, Eric Peng Huat
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349311/ https://www.ncbi.nlm.nih.gov/pubmed/32570810 http://dx.doi.org/10.3390/genes11060664

Ejemplares similares

Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification
por: An, Yi-Quan, et al.
Publicado: (2023)

Rapid PCR Thermocycling using Microscale Thermal Convection
por: Muddu, Radha, et al.
Publicado: (2011)

Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics
por: Gavrilov, Momčilo, et al.
Publicado: (2022)

A Thermocycler Using a Chip Resistor Heater and a Glass Microchip for a Portable and Rapid Microchip-Based PCR Device
por: Yeom, Dongsun, et al.
Publicado: (2022)

Towards PCB-Based Miniaturized Thermocyclers for DNA Amplification
por: Kaprou, Georgia D., et al.
Publicado: (2020)

A portable system for economical nucleic acid amplification testing
por: Dong, Hui, et al.
Publicado: (2023)

Draft Genome Sequence of Multidrug Resistant Klebsiella pneumoniae Strain C43 Isolated from Environmental Water Sample
por: Wee, Soon Keong, et al.
Publicado: (2022)

Paper microfluidics with deep learning for portable intelligent nucleic acid amplification tests
por: Sun, Hao, et al.
Publicado: (2023)

Droplet centrifugation, droplet DNA extraction, and rapid droplet thermocycling for simpler and faster PCR assay using wire-guided manipulations
por: You, David J, et al.
Publicado: (2012)

Validation of use of the miniPCR thermocycler for Ebola and Zika virus detection
por: González-González, Everardo, et al.
Publicado: (2019)

Portable and accurate diagnostics for COVID-19: Combined use of the miniPCR thermocycler and a well-plate reader for SARS-CoV-2 virus detection
por: González-González, Everardo, et al.
Publicado: (2020)

Portable and Battery-Powered PCR Device for DNA Amplification and Fluorescence Detection
por: Jie, Junyao, et al.
Publicado: (2020)

Accurate Estimation of Nucleic Acids by Amplification Efficiency Dependent PCR
por: Chatterjee, Nilanjana, et al.
Publicado: (2012)

Rapid Point-of-Care Isothermal Amplification Assay for the Detection of Malaria without Nucleic Acid Purification
por: Modak, Sayli S., et al.
Publicado: (2016)

Electrochemical molecular analysis without nucleic acid amplification
por: Gau, Vincent, et al.
Publicado: (2005)

An evaluation of direct PCR amplification
por: Hall, Daniel E., et al.
Publicado: (2014)

Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics
por: Toldrà, Anna, et al.
Publicado: (2022)

Rapid point of care testing for four bacterial sexually transmitted infections using the portable isothermal loop-mediated nucleic acid amplification eazyplex platform
por: Esitgen Germaner, Ece T., et al.
Publicado: (2023)

Resistance of PETG Materials on Thermocycling and Brushing
por: Šimunović, Luka, et al.
Publicado: (2023)

Locked Nucleic Acid Pentamers as Universal PCR Primers for Genomic DNA Amplification
por: Sun, Zhen, et al.
Publicado: (2008)

Rapid and Sensitive Assay of Helicobacter pylori With One-Tube RPA-CRISPR/Cas12 by Portable Array Detector for Visible Analysis of Thermostatic Nucleic Acid Amplification
por: Dai, Bing, et al.
Publicado: (2022)

Rapid isothermal amplification and portable detection system for SARS-CoV-2
por: Ganguli, Anurup, et al.
Publicado: (2020)

Rapid Isothermal Amplification and Portable Detection System for SARS-CoV-2
por: Ganguli, A., et al.
Publicado: (2020)

Draft Genome Sequence of Enterobacter hormaechei subsp. steigerwaltii Strain BEI01
por: Wee, Soon Keong, et al.
Publicado: (2021)

An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection
por: Deng, Hao, et al.
Publicado: (2021)

Analysis of the effect of thermocycling on bonding cements to zirconia
por: Alrabeah, Ghada, et al.
Publicado: (2023)

Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends
por: Zhang, Chunsun, et al.
Publicado: (2007)

Publisher Correction: Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device
por: Cheong, Jiyong, et al.
Publicado: (2020)

Innate Immune Responses of Pulmonary Epithelial Cells to Burkholderia pseudomallei Infection
por: Sim, Siew Hoon, et al.
Publicado: (2009)

Rapid diagnosis of Leishmania infection with a portable loop-mediated isothermal amplification device
por: Puri, Madhu, et al.
Publicado: (2021)

Real-Time PCR for detection of herpes simplex virus without nucleic acid extraction
por: Pandori, Mark W, et al.
Publicado: (2006)

Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting
por: Chen, Yanju, et al.
Publicado: (2020)

Effect of thermocycling on the flexural strength of porcelain laminate veneers
por: Subramanian, Deepa, et al.
Publicado: (2008)

Effect of Bleaching and Thermocycling on Resin-Enamel Bond Strength
por: Moosavi, Horieh, et al.
Publicado: (2015)

Portable Heating System Based on a Liquid Metal Bath for Rapid PCR
por: Wang, Kangning, et al.
Publicado: (2022)

Portable real-time colorimetric LAMP-device for rapid quantitative detection of nucleic acids in crude samples
por: Papadakis, G., et al.
Publicado: (2022)

Colony-PCR Is a Rapid Method for DNA Amplification of Hyphomycetes
por: Walch, Georg, et al.
Publicado: (2016)

Application and evaluation of nucleic acid sequence-based amplification, PCR and cryptococcal antigen test for diagnosis of cryptococcosis
por: Wang, Yanping, et al.
Publicado: (2021)

Rapid and Sensitive Isothermal Detection of Nucleic-acid Sequence by Multiple Cross Displacement Amplification
por: Wang, Yi, et al.
Publicado: (2015)

T‐Cup: A Cheap, Rapid, and Simple Home Device for Isothermal Nucleic Acid Amplification
por: Velders, Aldrik H., et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_fqer7asrrm8fnt207mbgj7qo51