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Human Liver-Derived Extracellular Matrix for the Culture of Distinct Human Primary Liver Cells
The lack of robust methods to preserve, purify and in vitro maintain the phenotype of the human liver’s highly specialized parenchymal and non-parenchymal cell types importantly hampers their exploitation for the development of research and clinical applications. There is in this regard a growing in...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349413/ https://www.ncbi.nlm.nih.gov/pubmed/32486126 http://dx.doi.org/10.3390/cells9061357 |
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author | Alevra Sarika, Niki Payen, Valéry L. Fléron, Maximilien Ravau, Joachim Brusa, Davide Najimi, Mustapha De Pauw, Edwin Eppe, Gauthier Mazzucchelli, Gabriel Sokal, Etienne M. des Rieux, Anne El Taghdouini, Adil |
author_facet | Alevra Sarika, Niki Payen, Valéry L. Fléron, Maximilien Ravau, Joachim Brusa, Davide Najimi, Mustapha De Pauw, Edwin Eppe, Gauthier Mazzucchelli, Gabriel Sokal, Etienne M. des Rieux, Anne El Taghdouini, Adil |
author_sort | Alevra Sarika, Niki |
collection | PubMed |
description | The lack of robust methods to preserve, purify and in vitro maintain the phenotype of the human liver’s highly specialized parenchymal and non-parenchymal cell types importantly hampers their exploitation for the development of research and clinical applications. There is in this regard a growing interest in the use of tissue-specific extracellular matrix (ECM) to provide cells with an in vitro environment that more closely resembles that of the native tissue. In the present study, we have developed a method that allows for the isolation and downstream application of the human liver’s main cell types from cryopreserved material. We also isolated and solubilized human liver ECM (HL-ECM), analyzed its peptidomic and proteomic composition by mass spectrometry and evaluated its interest for the culture of distinct primary human liver cells. Our analysis of the HL-ECM revealed proteomic diversity, type 1 collagen abundance and partial loss of integrity following solubilization. Solubilized HL-ECM was evaluated either as a coating or as a medium supplement for the culture of human primary hepatocytes, hepatic stellate cells and liver sinusoidal endothelial cells. Whereas the solubilized HL-ECM was suitable for cell culture, its impact on the phenotype and/or functionality of the human liver cells was limited. Our study provides a first detailed characterization of solubilized HL-ECM and a first report of its influence on the culture of distinct human primary liver cells. |
format | Online Article Text |
id | pubmed-7349413 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73494132020-07-14 Human Liver-Derived Extracellular Matrix for the Culture of Distinct Human Primary Liver Cells Alevra Sarika, Niki Payen, Valéry L. Fléron, Maximilien Ravau, Joachim Brusa, Davide Najimi, Mustapha De Pauw, Edwin Eppe, Gauthier Mazzucchelli, Gabriel Sokal, Etienne M. des Rieux, Anne El Taghdouini, Adil Cells Article The lack of robust methods to preserve, purify and in vitro maintain the phenotype of the human liver’s highly specialized parenchymal and non-parenchymal cell types importantly hampers their exploitation for the development of research and clinical applications. There is in this regard a growing interest in the use of tissue-specific extracellular matrix (ECM) to provide cells with an in vitro environment that more closely resembles that of the native tissue. In the present study, we have developed a method that allows for the isolation and downstream application of the human liver’s main cell types from cryopreserved material. We also isolated and solubilized human liver ECM (HL-ECM), analyzed its peptidomic and proteomic composition by mass spectrometry and evaluated its interest for the culture of distinct primary human liver cells. Our analysis of the HL-ECM revealed proteomic diversity, type 1 collagen abundance and partial loss of integrity following solubilization. Solubilized HL-ECM was evaluated either as a coating or as a medium supplement for the culture of human primary hepatocytes, hepatic stellate cells and liver sinusoidal endothelial cells. Whereas the solubilized HL-ECM was suitable for cell culture, its impact on the phenotype and/or functionality of the human liver cells was limited. Our study provides a first detailed characterization of solubilized HL-ECM and a first report of its influence on the culture of distinct human primary liver cells. MDPI 2020-05-30 /pmc/articles/PMC7349413/ /pubmed/32486126 http://dx.doi.org/10.3390/cells9061357 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Alevra Sarika, Niki Payen, Valéry L. Fléron, Maximilien Ravau, Joachim Brusa, Davide Najimi, Mustapha De Pauw, Edwin Eppe, Gauthier Mazzucchelli, Gabriel Sokal, Etienne M. des Rieux, Anne El Taghdouini, Adil Human Liver-Derived Extracellular Matrix for the Culture of Distinct Human Primary Liver Cells |
title | Human Liver-Derived Extracellular Matrix for the Culture of Distinct Human Primary Liver Cells |
title_full | Human Liver-Derived Extracellular Matrix for the Culture of Distinct Human Primary Liver Cells |
title_fullStr | Human Liver-Derived Extracellular Matrix for the Culture of Distinct Human Primary Liver Cells |
title_full_unstemmed | Human Liver-Derived Extracellular Matrix for the Culture of Distinct Human Primary Liver Cells |
title_short | Human Liver-Derived Extracellular Matrix for the Culture of Distinct Human Primary Liver Cells |
title_sort | human liver-derived extracellular matrix for the culture of distinct human primary liver cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349413/ https://www.ncbi.nlm.nih.gov/pubmed/32486126 http://dx.doi.org/10.3390/cells9061357 |
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