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Polysaccharide Multilayer Films in Sensors for Detecting Prostate Tumor Cells Based on Hyaluronan-CD44 Interactions

The increasing need for point-of-care diagnosis has sparked the development of label-free sensing platforms, some of which are based on impedance measurements with biological cells. Here, interdigitated electrodes were functionalized with layer-by-layer (LbL) films of hyaluronan (HA) and chitosan (C...

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Autores principales: Rocha Neto, João Batista Maia, Soares, Andrey Coatrini, Bataglioli, Rogério Aparecido, Carr, Olívia, Costa, Carlos Alberto Rodrigues, Oliveira, Osvaldo N., Beppu, Marisa Masumi, Carvalho, Hernandes F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349506/
https://www.ncbi.nlm.nih.gov/pubmed/32604896
http://dx.doi.org/10.3390/cells9061563
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author Rocha Neto, João Batista Maia
Soares, Andrey Coatrini
Bataglioli, Rogério Aparecido
Carr, Olívia
Costa, Carlos Alberto Rodrigues
Oliveira, Osvaldo N.
Beppu, Marisa Masumi
Carvalho, Hernandes F.
author_facet Rocha Neto, João Batista Maia
Soares, Andrey Coatrini
Bataglioli, Rogério Aparecido
Carr, Olívia
Costa, Carlos Alberto Rodrigues
Oliveira, Osvaldo N.
Beppu, Marisa Masumi
Carvalho, Hernandes F.
author_sort Rocha Neto, João Batista Maia
collection PubMed
description The increasing need for point-of-care diagnosis has sparked the development of label-free sensing platforms, some of which are based on impedance measurements with biological cells. Here, interdigitated electrodes were functionalized with layer-by-layer (LbL) films of hyaluronan (HA) and chitosan (CHI) to detect prostatic tumor cells (PC3 line). The deposition of LbL films was confirmed with atomic force microscopy and polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS), which featured the vibrational modes of the HA top layer capable of interacting specifically with glycoprotein CD44 receptors overexpressed in tumor cells. Though the CHI/HA LbL films cannot be considered as a traditional biosensor due to their limited selectivity, it was possible to distinguish prostate tumor cells in the range from 50 to 600 cells/µL in in vitro experiments with impedance spectroscopy. This was achieved by treating the impedance data with information visualization methods, which confirmed the distinguishing ability of the films by observing the absence of false positives in a series of control experiments. The CD44–HA interactions may, therefore, be exploited in clinical analyses and point-of-care diagnostics for cancer, particularly if computational methods are used to process the data.
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spelling pubmed-73495062020-07-14 Polysaccharide Multilayer Films in Sensors for Detecting Prostate Tumor Cells Based on Hyaluronan-CD44 Interactions Rocha Neto, João Batista Maia Soares, Andrey Coatrini Bataglioli, Rogério Aparecido Carr, Olívia Costa, Carlos Alberto Rodrigues Oliveira, Osvaldo N. Beppu, Marisa Masumi Carvalho, Hernandes F. Cells Article The increasing need for point-of-care diagnosis has sparked the development of label-free sensing platforms, some of which are based on impedance measurements with biological cells. Here, interdigitated electrodes were functionalized with layer-by-layer (LbL) films of hyaluronan (HA) and chitosan (CHI) to detect prostatic tumor cells (PC3 line). The deposition of LbL films was confirmed with atomic force microscopy and polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS), which featured the vibrational modes of the HA top layer capable of interacting specifically with glycoprotein CD44 receptors overexpressed in tumor cells. Though the CHI/HA LbL films cannot be considered as a traditional biosensor due to their limited selectivity, it was possible to distinguish prostate tumor cells in the range from 50 to 600 cells/µL in in vitro experiments with impedance spectroscopy. This was achieved by treating the impedance data with information visualization methods, which confirmed the distinguishing ability of the films by observing the absence of false positives in a series of control experiments. The CD44–HA interactions may, therefore, be exploited in clinical analyses and point-of-care diagnostics for cancer, particularly if computational methods are used to process the data. MDPI 2020-06-26 /pmc/articles/PMC7349506/ /pubmed/32604896 http://dx.doi.org/10.3390/cells9061563 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rocha Neto, João Batista Maia
Soares, Andrey Coatrini
Bataglioli, Rogério Aparecido
Carr, Olívia
Costa, Carlos Alberto Rodrigues
Oliveira, Osvaldo N.
Beppu, Marisa Masumi
Carvalho, Hernandes F.
Polysaccharide Multilayer Films in Sensors for Detecting Prostate Tumor Cells Based on Hyaluronan-CD44 Interactions
title Polysaccharide Multilayer Films in Sensors for Detecting Prostate Tumor Cells Based on Hyaluronan-CD44 Interactions
title_full Polysaccharide Multilayer Films in Sensors for Detecting Prostate Tumor Cells Based on Hyaluronan-CD44 Interactions
title_fullStr Polysaccharide Multilayer Films in Sensors for Detecting Prostate Tumor Cells Based on Hyaluronan-CD44 Interactions
title_full_unstemmed Polysaccharide Multilayer Films in Sensors for Detecting Prostate Tumor Cells Based on Hyaluronan-CD44 Interactions
title_short Polysaccharide Multilayer Films in Sensors for Detecting Prostate Tumor Cells Based on Hyaluronan-CD44 Interactions
title_sort polysaccharide multilayer films in sensors for detecting prostate tumor cells based on hyaluronan-cd44 interactions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349506/
https://www.ncbi.nlm.nih.gov/pubmed/32604896
http://dx.doi.org/10.3390/cells9061563
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