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Discovery of the Involvement in DNA Oxidative Damage of Human Sperm Nuclear Basic Proteins of Healthy Young Men Living in Polluted Areas

DNA oxidative damage is one of the main concerns being implicated in severe cell alterations, promoting different types of human disorders and diseases. For their characteristics, male gametes are the most sensitive cells to the accumulation of damaged DNA. We have recently reported the relevance of...

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Autores principales: Lettieri, Gennaro, D’Agostino, Giovanni, Mele, Elena, Cardito, Carolina, Esposito, Rosa, Cimmino, Annalinda, Giarra, Antonella, Trifuoggi, Marco, Raimondo, Salvatore, Notari, Tiziana, Febbraio, Ferdinando, Montano, Luigi, Piscopo, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349829/
https://www.ncbi.nlm.nih.gov/pubmed/32545547
http://dx.doi.org/10.3390/ijms21124198
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author Lettieri, Gennaro
D’Agostino, Giovanni
Mele, Elena
Cardito, Carolina
Esposito, Rosa
Cimmino, Annalinda
Giarra, Antonella
Trifuoggi, Marco
Raimondo, Salvatore
Notari, Tiziana
Febbraio, Ferdinando
Montano, Luigi
Piscopo, Marina
author_facet Lettieri, Gennaro
D’Agostino, Giovanni
Mele, Elena
Cardito, Carolina
Esposito, Rosa
Cimmino, Annalinda
Giarra, Antonella
Trifuoggi, Marco
Raimondo, Salvatore
Notari, Tiziana
Febbraio, Ferdinando
Montano, Luigi
Piscopo, Marina
author_sort Lettieri, Gennaro
collection PubMed
description DNA oxidative damage is one of the main concerns being implicated in severe cell alterations, promoting different types of human disorders and diseases. For their characteristics, male gametes are the most sensitive cells to the accumulation of damaged DNA. We have recently reported the relevance of arginine residues in the Cu(II)-induced DNA breakage of sperm H1 histones. In this work, we have extended our previous findings investigating the involvement of human sperm nuclear basic proteins on DNA oxidative damage in healthy males presenting copper and chromium excess in their semen. We found in 84% of those males an altered protamines/histones ratio and a different DNA binding mode even for those presenting a canonical protamines/histones ratio. Furthermore, all the sperm nuclear basic proteins from these samples that resulted were involved in DNA oxidative damage, supporting the idea that these proteins could promote the Fenton reaction in DNA proximity by increasing the availability of these metals near the binding surface of DNA. In conclusion, our study reveals a new and unexpected behavior of human sperm nuclear basic proteins in oxidative DNA damage, providing new insights for understanding the mechanisms related to processes in which oxidative DNA damage is implicated.
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spelling pubmed-73498292020-07-15 Discovery of the Involvement in DNA Oxidative Damage of Human Sperm Nuclear Basic Proteins of Healthy Young Men Living in Polluted Areas Lettieri, Gennaro D’Agostino, Giovanni Mele, Elena Cardito, Carolina Esposito, Rosa Cimmino, Annalinda Giarra, Antonella Trifuoggi, Marco Raimondo, Salvatore Notari, Tiziana Febbraio, Ferdinando Montano, Luigi Piscopo, Marina Int J Mol Sci Article DNA oxidative damage is one of the main concerns being implicated in severe cell alterations, promoting different types of human disorders and diseases. For their characteristics, male gametes are the most sensitive cells to the accumulation of damaged DNA. We have recently reported the relevance of arginine residues in the Cu(II)-induced DNA breakage of sperm H1 histones. In this work, we have extended our previous findings investigating the involvement of human sperm nuclear basic proteins on DNA oxidative damage in healthy males presenting copper and chromium excess in their semen. We found in 84% of those males an altered protamines/histones ratio and a different DNA binding mode even for those presenting a canonical protamines/histones ratio. Furthermore, all the sperm nuclear basic proteins from these samples that resulted were involved in DNA oxidative damage, supporting the idea that these proteins could promote the Fenton reaction in DNA proximity by increasing the availability of these metals near the binding surface of DNA. In conclusion, our study reveals a new and unexpected behavior of human sperm nuclear basic proteins in oxidative DNA damage, providing new insights for understanding the mechanisms related to processes in which oxidative DNA damage is implicated. MDPI 2020-06-12 /pmc/articles/PMC7349829/ /pubmed/32545547 http://dx.doi.org/10.3390/ijms21124198 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lettieri, Gennaro
D’Agostino, Giovanni
Mele, Elena
Cardito, Carolina
Esposito, Rosa
Cimmino, Annalinda
Giarra, Antonella
Trifuoggi, Marco
Raimondo, Salvatore
Notari, Tiziana
Febbraio, Ferdinando
Montano, Luigi
Piscopo, Marina
Discovery of the Involvement in DNA Oxidative Damage of Human Sperm Nuclear Basic Proteins of Healthy Young Men Living in Polluted Areas
title Discovery of the Involvement in DNA Oxidative Damage of Human Sperm Nuclear Basic Proteins of Healthy Young Men Living in Polluted Areas
title_full Discovery of the Involvement in DNA Oxidative Damage of Human Sperm Nuclear Basic Proteins of Healthy Young Men Living in Polluted Areas
title_fullStr Discovery of the Involvement in DNA Oxidative Damage of Human Sperm Nuclear Basic Proteins of Healthy Young Men Living in Polluted Areas
title_full_unstemmed Discovery of the Involvement in DNA Oxidative Damage of Human Sperm Nuclear Basic Proteins of Healthy Young Men Living in Polluted Areas
title_short Discovery of the Involvement in DNA Oxidative Damage of Human Sperm Nuclear Basic Proteins of Healthy Young Men Living in Polluted Areas
title_sort discovery of the involvement in dna oxidative damage of human sperm nuclear basic proteins of healthy young men living in polluted areas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349829/
https://www.ncbi.nlm.nih.gov/pubmed/32545547
http://dx.doi.org/10.3390/ijms21124198
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