Cargando…
Immunization with Bovine Serum Albumin (BSA) in Oil-Adjuvant Elicits IgM Antibody Response in Chinese Soft-Shelled Turtle (Pelodiscus Sinensis)
Immunoassays are among the frontline methods used for disease diagnosis and surveillance. Despite this, there are no immunoassays developed for the Chinese soft-shelled turtle (Pelodiscus sinensis), which has expanded into large scale commercial production in several Asian countries. One of the crit...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349957/ https://www.ncbi.nlm.nih.gov/pubmed/32485925 http://dx.doi.org/10.3390/vaccines8020257 |
_version_ | 1783557174933323776 |
---|---|
author | Xu, Cheng Xu, Jiehao Chen, Yu Evensen, Øystein Munang’andu, Hetron Mweemba Qian, Guoying |
author_facet | Xu, Cheng Xu, Jiehao Chen, Yu Evensen, Øystein Munang’andu, Hetron Mweemba Qian, Guoying |
author_sort | Xu, Cheng |
collection | PubMed |
description | Immunoassays are among the frontline methods used for disease diagnosis and surveillance. Despite this, there are no immunoassays developed for the Chinese soft-shelled turtle (Pelodiscus sinensis), which has expanded into large scale commercial production in several Asian countries. One of the critical factors delaying the development of immunoassays is the lack of characterized soft-shelled turtle immunoglobulins. Herein, we used mass spectrometry together with the ProtQuest software to identify the soft-shelled turtle IgM heavy chain in serum, which again was used to produce a polyclonal anti-turtle-IgM in rabbits. Thereafter, the polyclonal anti-turtle-IgM was used as a secondary antibody in an indirect ELISA to evaluate antibody responses of soft-shelled turtles injected with the bovine serum albumin (BSA) model antigen. Our findings show that only turtle immunized with a water-in-oil BSA plus ISA 763A VG adjuvant (SEPPIC, France) emulsion had antibodies detected at 42 days post vaccination (dpv) while turtles injected with phosphate buffered saline (PBS) only as well as turtle injected with BSA dissolved in PBS had no significant antibody levels detected in serum throughout the study period. In summary, our findings show that rabbit polyclonal anti-turtle-IgM produced can be used in ELISA to measure serum antibody responses in immunized soft-shelled turtles. Future studies should explore its application in other immunoassays needed for the disease diagnosis and vaccine development for soft-shelled turtles. |
format | Online Article Text |
id | pubmed-7349957 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73499572020-07-22 Immunization with Bovine Serum Albumin (BSA) in Oil-Adjuvant Elicits IgM Antibody Response in Chinese Soft-Shelled Turtle (Pelodiscus Sinensis) Xu, Cheng Xu, Jiehao Chen, Yu Evensen, Øystein Munang’andu, Hetron Mweemba Qian, Guoying Vaccines (Basel) Article Immunoassays are among the frontline methods used for disease diagnosis and surveillance. Despite this, there are no immunoassays developed for the Chinese soft-shelled turtle (Pelodiscus sinensis), which has expanded into large scale commercial production in several Asian countries. One of the critical factors delaying the development of immunoassays is the lack of characterized soft-shelled turtle immunoglobulins. Herein, we used mass spectrometry together with the ProtQuest software to identify the soft-shelled turtle IgM heavy chain in serum, which again was used to produce a polyclonal anti-turtle-IgM in rabbits. Thereafter, the polyclonal anti-turtle-IgM was used as a secondary antibody in an indirect ELISA to evaluate antibody responses of soft-shelled turtles injected with the bovine serum albumin (BSA) model antigen. Our findings show that only turtle immunized with a water-in-oil BSA plus ISA 763A VG adjuvant (SEPPIC, France) emulsion had antibodies detected at 42 days post vaccination (dpv) while turtles injected with phosphate buffered saline (PBS) only as well as turtle injected with BSA dissolved in PBS had no significant antibody levels detected in serum throughout the study period. In summary, our findings show that rabbit polyclonal anti-turtle-IgM produced can be used in ELISA to measure serum antibody responses in immunized soft-shelled turtles. Future studies should explore its application in other immunoassays needed for the disease diagnosis and vaccine development for soft-shelled turtles. MDPI 2020-05-29 /pmc/articles/PMC7349957/ /pubmed/32485925 http://dx.doi.org/10.3390/vaccines8020257 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xu, Cheng Xu, Jiehao Chen, Yu Evensen, Øystein Munang’andu, Hetron Mweemba Qian, Guoying Immunization with Bovine Serum Albumin (BSA) in Oil-Adjuvant Elicits IgM Antibody Response in Chinese Soft-Shelled Turtle (Pelodiscus Sinensis) |
title | Immunization with Bovine Serum Albumin (BSA) in Oil-Adjuvant Elicits IgM Antibody Response in Chinese Soft-Shelled Turtle (Pelodiscus Sinensis) |
title_full | Immunization with Bovine Serum Albumin (BSA) in Oil-Adjuvant Elicits IgM Antibody Response in Chinese Soft-Shelled Turtle (Pelodiscus Sinensis) |
title_fullStr | Immunization with Bovine Serum Albumin (BSA) in Oil-Adjuvant Elicits IgM Antibody Response in Chinese Soft-Shelled Turtle (Pelodiscus Sinensis) |
title_full_unstemmed | Immunization with Bovine Serum Albumin (BSA) in Oil-Adjuvant Elicits IgM Antibody Response in Chinese Soft-Shelled Turtle (Pelodiscus Sinensis) |
title_short | Immunization with Bovine Serum Albumin (BSA) in Oil-Adjuvant Elicits IgM Antibody Response in Chinese Soft-Shelled Turtle (Pelodiscus Sinensis) |
title_sort | immunization with bovine serum albumin (bsa) in oil-adjuvant elicits igm antibody response in chinese soft-shelled turtle (pelodiscus sinensis) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349957/ https://www.ncbi.nlm.nih.gov/pubmed/32485925 http://dx.doi.org/10.3390/vaccines8020257 |
work_keys_str_mv | AT xucheng immunizationwithbovineserumalbuminbsainoiladjuvantelicitsigmantibodyresponseinchinesesoftshelledturtlepelodiscussinensis AT xujiehao immunizationwithbovineserumalbuminbsainoiladjuvantelicitsigmantibodyresponseinchinesesoftshelledturtlepelodiscussinensis AT chenyu immunizationwithbovineserumalbuminbsainoiladjuvantelicitsigmantibodyresponseinchinesesoftshelledturtlepelodiscussinensis AT evensenøystein immunizationwithbovineserumalbuminbsainoiladjuvantelicitsigmantibodyresponseinchinesesoftshelledturtlepelodiscussinensis AT munanganduhetronmweemba immunizationwithbovineserumalbuminbsainoiladjuvantelicitsigmantibodyresponseinchinesesoftshelledturtlepelodiscussinensis AT qianguoying immunizationwithbovineserumalbuminbsainoiladjuvantelicitsigmantibodyresponseinchinesesoftshelledturtlepelodiscussinensis |