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Establishing a Robust Manufacturing Platform for Recombinant Veterinary Vaccines: An Adenovirus-Vector Vaccine to Control Newcastle Disease Virus Infections of Poultry in Sub-Saharan Africa

Developing vaccine technology platforms to respond to pandemic threats or zoonotic diseases is a worldwide high priority. The risk of infectious diseases transmitted from wildlife and domestic animals to humans makes veterinary vaccination and animal health monitoring highly relevant for the deploym...

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Autores principales: Farnós, Omar, Gelaye, Esayas, Trabelsi, Khaled, Bernier, Alice, Subramani, Kumar, Kallel, Héla, Yami, Martha, Kamen, Amine A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350225/
https://www.ncbi.nlm.nih.gov/pubmed/32604755
http://dx.doi.org/10.3390/vaccines8020338
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author Farnós, Omar
Gelaye, Esayas
Trabelsi, Khaled
Bernier, Alice
Subramani, Kumar
Kallel, Héla
Yami, Martha
Kamen, Amine A.
author_facet Farnós, Omar
Gelaye, Esayas
Trabelsi, Khaled
Bernier, Alice
Subramani, Kumar
Kallel, Héla
Yami, Martha
Kamen, Amine A.
author_sort Farnós, Omar
collection PubMed
description Developing vaccine technology platforms to respond to pandemic threats or zoonotic diseases is a worldwide high priority. The risk of infectious diseases transmitted from wildlife and domestic animals to humans makes veterinary vaccination and animal health monitoring highly relevant for the deployment of public health global policies in the context of “one world, one health” principles. Sub-Saharan Africa is frequently impacted by outbreaks of poultry diseases such as avian influenza and Newcastle Disease (ND). Here, an adenovirus-vectored vaccine technology platform is proposed for rapid adaptation to ND or other avian viral threats in the region. Ethiopian isolates of the Newcastle Disease virus (NDV) were subjected to sequence and phylogenetic analyses, enabling the construction of antigenically matched vaccine candidates expressing the fusion (F) and hemagglutinin-neuraminidase (HN) proteins. A cost-effective vaccine production process was developed using HEK293 cells in suspension and serum-free medium. Productive infection in bioreactors (1–3 L) at 2 × 10(6) cells/mL resulted in consistent infectious adenoviral vector titers of approximately 5–6 × 10(8) TCID(50)/mL (approximately 10(11)VP/mL) in the harvest lysates. Groups of chickens were twice immunized with 1 × 10(10) TCID(50) of the vectors, and full protection against a lethal NDV challenge was provided by the vector expressing the F antigen. These results consolidate the basis for a streamlined and scalable-vectored vaccine manufacturing process for deployment in low- and medium-income countries.
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spelling pubmed-73502252020-07-22 Establishing a Robust Manufacturing Platform for Recombinant Veterinary Vaccines: An Adenovirus-Vector Vaccine to Control Newcastle Disease Virus Infections of Poultry in Sub-Saharan Africa Farnós, Omar Gelaye, Esayas Trabelsi, Khaled Bernier, Alice Subramani, Kumar Kallel, Héla Yami, Martha Kamen, Amine A. Vaccines (Basel) Article Developing vaccine technology platforms to respond to pandemic threats or zoonotic diseases is a worldwide high priority. The risk of infectious diseases transmitted from wildlife and domestic animals to humans makes veterinary vaccination and animal health monitoring highly relevant for the deployment of public health global policies in the context of “one world, one health” principles. Sub-Saharan Africa is frequently impacted by outbreaks of poultry diseases such as avian influenza and Newcastle Disease (ND). Here, an adenovirus-vectored vaccine technology platform is proposed for rapid adaptation to ND or other avian viral threats in the region. Ethiopian isolates of the Newcastle Disease virus (NDV) were subjected to sequence and phylogenetic analyses, enabling the construction of antigenically matched vaccine candidates expressing the fusion (F) and hemagglutinin-neuraminidase (HN) proteins. A cost-effective vaccine production process was developed using HEK293 cells in suspension and serum-free medium. Productive infection in bioreactors (1–3 L) at 2 × 10(6) cells/mL resulted in consistent infectious adenoviral vector titers of approximately 5–6 × 10(8) TCID(50)/mL (approximately 10(11)VP/mL) in the harvest lysates. Groups of chickens were twice immunized with 1 × 10(10) TCID(50) of the vectors, and full protection against a lethal NDV challenge was provided by the vector expressing the F antigen. These results consolidate the basis for a streamlined and scalable-vectored vaccine manufacturing process for deployment in low- and medium-income countries. MDPI 2020-06-26 /pmc/articles/PMC7350225/ /pubmed/32604755 http://dx.doi.org/10.3390/vaccines8020338 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Farnós, Omar
Gelaye, Esayas
Trabelsi, Khaled
Bernier, Alice
Subramani, Kumar
Kallel, Héla
Yami, Martha
Kamen, Amine A.
Establishing a Robust Manufacturing Platform for Recombinant Veterinary Vaccines: An Adenovirus-Vector Vaccine to Control Newcastle Disease Virus Infections of Poultry in Sub-Saharan Africa
title Establishing a Robust Manufacturing Platform for Recombinant Veterinary Vaccines: An Adenovirus-Vector Vaccine to Control Newcastle Disease Virus Infections of Poultry in Sub-Saharan Africa
title_full Establishing a Robust Manufacturing Platform for Recombinant Veterinary Vaccines: An Adenovirus-Vector Vaccine to Control Newcastle Disease Virus Infections of Poultry in Sub-Saharan Africa
title_fullStr Establishing a Robust Manufacturing Platform for Recombinant Veterinary Vaccines: An Adenovirus-Vector Vaccine to Control Newcastle Disease Virus Infections of Poultry in Sub-Saharan Africa
title_full_unstemmed Establishing a Robust Manufacturing Platform for Recombinant Veterinary Vaccines: An Adenovirus-Vector Vaccine to Control Newcastle Disease Virus Infections of Poultry in Sub-Saharan Africa
title_short Establishing a Robust Manufacturing Platform for Recombinant Veterinary Vaccines: An Adenovirus-Vector Vaccine to Control Newcastle Disease Virus Infections of Poultry in Sub-Saharan Africa
title_sort establishing a robust manufacturing platform for recombinant veterinary vaccines: an adenovirus-vector vaccine to control newcastle disease virus infections of poultry in sub-saharan africa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350225/
https://www.ncbi.nlm.nih.gov/pubmed/32604755
http://dx.doi.org/10.3390/vaccines8020338
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