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Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental Aspergillus Infection in Poultry

Naturally occurring human antibodies (Abs) of the isotypes IgM and IgG and reactive to the galactose-α-1,3-galactose (α-Gal) epitope are associated with protection against infectious diseases, caused by pathogens expressing the glycan. Gut microbiota bacteria expressing α-Gal regulate the immune res...

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Autores principales: Mateos-Hernández, Lourdes, Risco-Castillo, Veronica, Torres-Maravilla, Edgar, Bermúdez-Humarán, Luis G., Alberdi, Pilar, Hodžić, Adnan, Hernández-Jarguin, Angelica, Rakotobe, Sabine, Galon, Clemence, Devillers, Elodie, de la Fuente, Jose, Guillot, Jacques, Cabezas-Cruz, Alejandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350254/
https://www.ncbi.nlm.nih.gov/pubmed/32517302
http://dx.doi.org/10.3390/vaccines8020285
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author Mateos-Hernández, Lourdes
Risco-Castillo, Veronica
Torres-Maravilla, Edgar
Bermúdez-Humarán, Luis G.
Alberdi, Pilar
Hodžić, Adnan
Hernández-Jarguin, Angelica
Rakotobe, Sabine
Galon, Clemence
Devillers, Elodie
de la Fuente, Jose
Guillot, Jacques
Cabezas-Cruz, Alejandro
author_facet Mateos-Hernández, Lourdes
Risco-Castillo, Veronica
Torres-Maravilla, Edgar
Bermúdez-Humarán, Luis G.
Alberdi, Pilar
Hodžić, Adnan
Hernández-Jarguin, Angelica
Rakotobe, Sabine
Galon, Clemence
Devillers, Elodie
de la Fuente, Jose
Guillot, Jacques
Cabezas-Cruz, Alejandro
author_sort Mateos-Hernández, Lourdes
collection PubMed
description Naturally occurring human antibodies (Abs) of the isotypes IgM and IgG and reactive to the galactose-α-1,3-galactose (α-Gal) epitope are associated with protection against infectious diseases, caused by pathogens expressing the glycan. Gut microbiota bacteria expressing α-Gal regulate the immune response to this glycan in animals lacking endogenous α-Gal. Here, we asked whether the production of anti-α-Gal Abs in response to microbiota stimulation in birds, confers protection against infection by Aspergillus fumigatus, a major fungal pathogen that expresses α-Gal in its surface. We demonstrated that the oral administration of Escherichia coli O86:B7 strain, a bacterium with high α-Gal content, reduces the occurrence of granulomas in lungs and protects turkeys from developing acute aspergillosis. Surprisingly, the protective effect of E. coli O86:B7 was not associated with an increase in circulating anti-α-Gal IgY levels, but with a striking reduction of anti-α-Gal IgA in the lungs of infected turkeys. Subcutaneous immunization against α-Gal did not induce a significant reduction of lung anti-α-Gal IgA and failed to protect against an infectious challenge with A. fumigatus. Oral administration of E. coli O86:B7 was not associated with the upregulation of lung cytokines upon A. fumigatus infection. We concluded that the oral administration of bacteria expressing high levels of α-Gal decreases the levels of lung anti-α-Gal IgA, which are mediators of inflammation and lung damage during acute aspergillosis.
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spelling pubmed-73502542020-07-22 Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental Aspergillus Infection in Poultry Mateos-Hernández, Lourdes Risco-Castillo, Veronica Torres-Maravilla, Edgar Bermúdez-Humarán, Luis G. Alberdi, Pilar Hodžić, Adnan Hernández-Jarguin, Angelica Rakotobe, Sabine Galon, Clemence Devillers, Elodie de la Fuente, Jose Guillot, Jacques Cabezas-Cruz, Alejandro Vaccines (Basel) Article Naturally occurring human antibodies (Abs) of the isotypes IgM and IgG and reactive to the galactose-α-1,3-galactose (α-Gal) epitope are associated with protection against infectious diseases, caused by pathogens expressing the glycan. Gut microbiota bacteria expressing α-Gal regulate the immune response to this glycan in animals lacking endogenous α-Gal. Here, we asked whether the production of anti-α-Gal Abs in response to microbiota stimulation in birds, confers protection against infection by Aspergillus fumigatus, a major fungal pathogen that expresses α-Gal in its surface. We demonstrated that the oral administration of Escherichia coli O86:B7 strain, a bacterium with high α-Gal content, reduces the occurrence of granulomas in lungs and protects turkeys from developing acute aspergillosis. Surprisingly, the protective effect of E. coli O86:B7 was not associated with an increase in circulating anti-α-Gal IgY levels, but with a striking reduction of anti-α-Gal IgA in the lungs of infected turkeys. Subcutaneous immunization against α-Gal did not induce a significant reduction of lung anti-α-Gal IgA and failed to protect against an infectious challenge with A. fumigatus. Oral administration of E. coli O86:B7 was not associated with the upregulation of lung cytokines upon A. fumigatus infection. We concluded that the oral administration of bacteria expressing high levels of α-Gal decreases the levels of lung anti-α-Gal IgA, which are mediators of inflammation and lung damage during acute aspergillosis. MDPI 2020-06-07 /pmc/articles/PMC7350254/ /pubmed/32517302 http://dx.doi.org/10.3390/vaccines8020285 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mateos-Hernández, Lourdes
Risco-Castillo, Veronica
Torres-Maravilla, Edgar
Bermúdez-Humarán, Luis G.
Alberdi, Pilar
Hodžić, Adnan
Hernández-Jarguin, Angelica
Rakotobe, Sabine
Galon, Clemence
Devillers, Elodie
de la Fuente, Jose
Guillot, Jacques
Cabezas-Cruz, Alejandro
Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental Aspergillus Infection in Poultry
title Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental Aspergillus Infection in Poultry
title_full Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental Aspergillus Infection in Poultry
title_fullStr Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental Aspergillus Infection in Poultry
title_full_unstemmed Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental Aspergillus Infection in Poultry
title_short Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental Aspergillus Infection in Poultry
title_sort gut microbiota abrogates anti-α-gal iga response in lungs and protects against experimental aspergillus infection in poultry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350254/
https://www.ncbi.nlm.nih.gov/pubmed/32517302
http://dx.doi.org/10.3390/vaccines8020285
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