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Detection of Foot-and-Mouth Disease Virus in Swine Meat Juice

Foot-and-mouth disease virus (FMDV) is a highly contagious agent that impacts livestock industries worldwide, leading to significant financial loss. Its impact can be avoided or minimized if the virus is detected early. FMDV detection relies on vesicular fluid, epithelial tags, swabs, serum, and oth...

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Detalles Bibliográficos
Autores principales: Yeo, Sean, Yang, Ming, Nyachoti, Martin, Rauh, Rolf, Callahan, Johnny D., Nfon, Charles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350341/
https://www.ncbi.nlm.nih.gov/pubmed/32485851
http://dx.doi.org/10.3390/pathogens9060424
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author Yeo, Sean
Yang, Ming
Nyachoti, Martin
Rauh, Rolf
Callahan, Johnny D.
Nfon, Charles
author_facet Yeo, Sean
Yang, Ming
Nyachoti, Martin
Rauh, Rolf
Callahan, Johnny D.
Nfon, Charles
author_sort Yeo, Sean
collection PubMed
description Foot-and-mouth disease virus (FMDV) is a highly contagious agent that impacts livestock industries worldwide, leading to significant financial loss. Its impact can be avoided or minimized if the virus is detected early. FMDV detection relies on vesicular fluid, epithelial tags, swabs, serum, and other sample types from live animals. These samples might not always be available, necessitating the use of alternative sample types. Meat juice (MJ), collected after freeze-thaw cycles of skeletal muscle, is a potential sample type for FMDV detection, especially when meat is illegally imported. We have performed experiments to evaluate the suitability of MJ for FMDV detection. MJ was collected from pigs that were experimentally infected with FMDV. Ribonucleic acid (RNA) was extracted from MJ, sera, oral swabs, and lymph nodes from the same animals and tested for FMDV by real-time reverse transcription polymerase chain reaction (rRT-PCR). MJ was also tested for FMDV antigen by Lateral Flow Immunoassay (LFI). FMDV RNA was detected in MJ by rRT-PCR starting at one day post infection (DPI) and as late as 21 DPI. In contrast, FMDV RNA was detected in sera at 1–7 DPI. Antigen was also detected in MJ at 1–9 DPI by LFI. Live virus was not isolated directly from MJ, but was recovered from the viral genome by transfection into susceptible cells. The data show that MJ is a good sample type for FMDV detection.
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spelling pubmed-73503412020-07-15 Detection of Foot-and-Mouth Disease Virus in Swine Meat Juice Yeo, Sean Yang, Ming Nyachoti, Martin Rauh, Rolf Callahan, Johnny D. Nfon, Charles Pathogens Article Foot-and-mouth disease virus (FMDV) is a highly contagious agent that impacts livestock industries worldwide, leading to significant financial loss. Its impact can be avoided or minimized if the virus is detected early. FMDV detection relies on vesicular fluid, epithelial tags, swabs, serum, and other sample types from live animals. These samples might not always be available, necessitating the use of alternative sample types. Meat juice (MJ), collected after freeze-thaw cycles of skeletal muscle, is a potential sample type for FMDV detection, especially when meat is illegally imported. We have performed experiments to evaluate the suitability of MJ for FMDV detection. MJ was collected from pigs that were experimentally infected with FMDV. Ribonucleic acid (RNA) was extracted from MJ, sera, oral swabs, and lymph nodes from the same animals and tested for FMDV by real-time reverse transcription polymerase chain reaction (rRT-PCR). MJ was also tested for FMDV antigen by Lateral Flow Immunoassay (LFI). FMDV RNA was detected in MJ by rRT-PCR starting at one day post infection (DPI) and as late as 21 DPI. In contrast, FMDV RNA was detected in sera at 1–7 DPI. Antigen was also detected in MJ at 1–9 DPI by LFI. Live virus was not isolated directly from MJ, but was recovered from the viral genome by transfection into susceptible cells. The data show that MJ is a good sample type for FMDV detection. MDPI 2020-05-29 /pmc/articles/PMC7350341/ /pubmed/32485851 http://dx.doi.org/10.3390/pathogens9060424 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yeo, Sean
Yang, Ming
Nyachoti, Martin
Rauh, Rolf
Callahan, Johnny D.
Nfon, Charles
Detection of Foot-and-Mouth Disease Virus in Swine Meat Juice
title Detection of Foot-and-Mouth Disease Virus in Swine Meat Juice
title_full Detection of Foot-and-Mouth Disease Virus in Swine Meat Juice
title_fullStr Detection of Foot-and-Mouth Disease Virus in Swine Meat Juice
title_full_unstemmed Detection of Foot-and-Mouth Disease Virus in Swine Meat Juice
title_short Detection of Foot-and-Mouth Disease Virus in Swine Meat Juice
title_sort detection of foot-and-mouth disease virus in swine meat juice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350341/
https://www.ncbi.nlm.nih.gov/pubmed/32485851
http://dx.doi.org/10.3390/pathogens9060424
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