Cargando…

In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress

Yeasts can adapt to a wide range of pH fluctuations (2 to 10), while Helicobacter pylori, a facultative intracellular bacterium, can adapt to a range from pH 6 to 8. This work analyzed if H. pylori J99 can protect itself from acidic pH by entering into Candida albicans ATCC 90028. Growth curves were...

Descripción completa

Detalles Bibliográficos
Autores principales: Sánchez-Alonzo, Kimberly, Parra-Sepúlveda, Cristian, Vega, Samuel, Bernasconi, Humberto, Campos, Víctor L., Smith, Carlos T., Sáez, Katia, García-Cancino, Apolinaria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350375/
https://www.ncbi.nlm.nih.gov/pubmed/32575493
http://dx.doi.org/10.3390/pathogens9060489
_version_ 1783557255237468160
author Sánchez-Alonzo, Kimberly
Parra-Sepúlveda, Cristian
Vega, Samuel
Bernasconi, Humberto
Campos, Víctor L.
Smith, Carlos T.
Sáez, Katia
García-Cancino, Apolinaria
author_facet Sánchez-Alonzo, Kimberly
Parra-Sepúlveda, Cristian
Vega, Samuel
Bernasconi, Humberto
Campos, Víctor L.
Smith, Carlos T.
Sáez, Katia
García-Cancino, Apolinaria
author_sort Sánchez-Alonzo, Kimberly
collection PubMed
description Yeasts can adapt to a wide range of pH fluctuations (2 to 10), while Helicobacter pylori, a facultative intracellular bacterium, can adapt to a range from pH 6 to 8. This work analyzed if H. pylori J99 can protect itself from acidic pH by entering into Candida albicans ATCC 90028. Growth curves were determined for H. pylori and C. albicans at pH 3, 4, and 7. Both microorganisms were co-incubated at the same pH values, and the presence of intra-yeast bacteria was evaluated. Intra-yeast bacteria-like bodies were detected using wet mounting, and intra-yeast binding of anti-H. pylori antibodies was detected using immunofluorescence. The presence of the H. pylori rDNA 16S gene in total DNA from yeasts was demonstrated after PCR amplification. H. pylori showed larger death percentages at pH 3 and 4 than at pH 7. On the contrary, the viability of the yeast was not affected by any of the pHs evaluated. H. pylori entered into C. albicans at all the pH values assayed but to a greater extent at unfavorable pH values (pH 3 or 4, p = 0.014 and p = 0.001, respectively). In conclusion, it is possible to suggest that H. pylori can shelter itself within C. albicans under unfavorable pH conditions.
format Online
Article
Text
id pubmed-7350375
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73503752020-07-15 In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress Sánchez-Alonzo, Kimberly Parra-Sepúlveda, Cristian Vega, Samuel Bernasconi, Humberto Campos, Víctor L. Smith, Carlos T. Sáez, Katia García-Cancino, Apolinaria Pathogens Article Yeasts can adapt to a wide range of pH fluctuations (2 to 10), while Helicobacter pylori, a facultative intracellular bacterium, can adapt to a range from pH 6 to 8. This work analyzed if H. pylori J99 can protect itself from acidic pH by entering into Candida albicans ATCC 90028. Growth curves were determined for H. pylori and C. albicans at pH 3, 4, and 7. Both microorganisms were co-incubated at the same pH values, and the presence of intra-yeast bacteria was evaluated. Intra-yeast bacteria-like bodies were detected using wet mounting, and intra-yeast binding of anti-H. pylori antibodies was detected using immunofluorescence. The presence of the H. pylori rDNA 16S gene in total DNA from yeasts was demonstrated after PCR amplification. H. pylori showed larger death percentages at pH 3 and 4 than at pH 7. On the contrary, the viability of the yeast was not affected by any of the pHs evaluated. H. pylori entered into C. albicans at all the pH values assayed but to a greater extent at unfavorable pH values (pH 3 or 4, p = 0.014 and p = 0.001, respectively). In conclusion, it is possible to suggest that H. pylori can shelter itself within C. albicans under unfavorable pH conditions. MDPI 2020-06-19 /pmc/articles/PMC7350375/ /pubmed/32575493 http://dx.doi.org/10.3390/pathogens9060489 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sánchez-Alonzo, Kimberly
Parra-Sepúlveda, Cristian
Vega, Samuel
Bernasconi, Humberto
Campos, Víctor L.
Smith, Carlos T.
Sáez, Katia
García-Cancino, Apolinaria
In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress
title In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress
title_full In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress
title_fullStr In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress
title_full_unstemmed In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress
title_short In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress
title_sort in vitro incorporation of helicobacter pylori into candida albicans caused by acidic ph stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350375/
https://www.ncbi.nlm.nih.gov/pubmed/32575493
http://dx.doi.org/10.3390/pathogens9060489
work_keys_str_mv AT sanchezalonzokimberly invitroincorporationofhelicobacterpyloriintocandidaalbicanscausedbyacidicphstress
AT parrasepulvedacristian invitroincorporationofhelicobacterpyloriintocandidaalbicanscausedbyacidicphstress
AT vegasamuel invitroincorporationofhelicobacterpyloriintocandidaalbicanscausedbyacidicphstress
AT bernasconihumberto invitroincorporationofhelicobacterpyloriintocandidaalbicanscausedbyacidicphstress
AT camposvictorl invitroincorporationofhelicobacterpyloriintocandidaalbicanscausedbyacidicphstress
AT smithcarlost invitroincorporationofhelicobacterpyloriintocandidaalbicanscausedbyacidicphstress
AT saezkatia invitroincorporationofhelicobacterpyloriintocandidaalbicanscausedbyacidicphstress
AT garciacancinoapolinaria invitroincorporationofhelicobacterpyloriintocandidaalbicanscausedbyacidicphstress