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In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress
Yeasts can adapt to a wide range of pH fluctuations (2 to 10), while Helicobacter pylori, a facultative intracellular bacterium, can adapt to a range from pH 6 to 8. This work analyzed if H. pylori J99 can protect itself from acidic pH by entering into Candida albicans ATCC 90028. Growth curves were...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350375/ https://www.ncbi.nlm.nih.gov/pubmed/32575493 http://dx.doi.org/10.3390/pathogens9060489 |
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author | Sánchez-Alonzo, Kimberly Parra-Sepúlveda, Cristian Vega, Samuel Bernasconi, Humberto Campos, Víctor L. Smith, Carlos T. Sáez, Katia García-Cancino, Apolinaria |
author_facet | Sánchez-Alonzo, Kimberly Parra-Sepúlveda, Cristian Vega, Samuel Bernasconi, Humberto Campos, Víctor L. Smith, Carlos T. Sáez, Katia García-Cancino, Apolinaria |
author_sort | Sánchez-Alonzo, Kimberly |
collection | PubMed |
description | Yeasts can adapt to a wide range of pH fluctuations (2 to 10), while Helicobacter pylori, a facultative intracellular bacterium, can adapt to a range from pH 6 to 8. This work analyzed if H. pylori J99 can protect itself from acidic pH by entering into Candida albicans ATCC 90028. Growth curves were determined for H. pylori and C. albicans at pH 3, 4, and 7. Both microorganisms were co-incubated at the same pH values, and the presence of intra-yeast bacteria was evaluated. Intra-yeast bacteria-like bodies were detected using wet mounting, and intra-yeast binding of anti-H. pylori antibodies was detected using immunofluorescence. The presence of the H. pylori rDNA 16S gene in total DNA from yeasts was demonstrated after PCR amplification. H. pylori showed larger death percentages at pH 3 and 4 than at pH 7. On the contrary, the viability of the yeast was not affected by any of the pHs evaluated. H. pylori entered into C. albicans at all the pH values assayed but to a greater extent at unfavorable pH values (pH 3 or 4, p = 0.014 and p = 0.001, respectively). In conclusion, it is possible to suggest that H. pylori can shelter itself within C. albicans under unfavorable pH conditions. |
format | Online Article Text |
id | pubmed-7350375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73503752020-07-15 In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress Sánchez-Alonzo, Kimberly Parra-Sepúlveda, Cristian Vega, Samuel Bernasconi, Humberto Campos, Víctor L. Smith, Carlos T. Sáez, Katia García-Cancino, Apolinaria Pathogens Article Yeasts can adapt to a wide range of pH fluctuations (2 to 10), while Helicobacter pylori, a facultative intracellular bacterium, can adapt to a range from pH 6 to 8. This work analyzed if H. pylori J99 can protect itself from acidic pH by entering into Candida albicans ATCC 90028. Growth curves were determined for H. pylori and C. albicans at pH 3, 4, and 7. Both microorganisms were co-incubated at the same pH values, and the presence of intra-yeast bacteria was evaluated. Intra-yeast bacteria-like bodies were detected using wet mounting, and intra-yeast binding of anti-H. pylori antibodies was detected using immunofluorescence. The presence of the H. pylori rDNA 16S gene in total DNA from yeasts was demonstrated after PCR amplification. H. pylori showed larger death percentages at pH 3 and 4 than at pH 7. On the contrary, the viability of the yeast was not affected by any of the pHs evaluated. H. pylori entered into C. albicans at all the pH values assayed but to a greater extent at unfavorable pH values (pH 3 or 4, p = 0.014 and p = 0.001, respectively). In conclusion, it is possible to suggest that H. pylori can shelter itself within C. albicans under unfavorable pH conditions. MDPI 2020-06-19 /pmc/articles/PMC7350375/ /pubmed/32575493 http://dx.doi.org/10.3390/pathogens9060489 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sánchez-Alonzo, Kimberly Parra-Sepúlveda, Cristian Vega, Samuel Bernasconi, Humberto Campos, Víctor L. Smith, Carlos T. Sáez, Katia García-Cancino, Apolinaria In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress |
title | In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress |
title_full | In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress |
title_fullStr | In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress |
title_full_unstemmed | In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress |
title_short | In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress |
title_sort | in vitro incorporation of helicobacter pylori into candida albicans caused by acidic ph stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350375/ https://www.ncbi.nlm.nih.gov/pubmed/32575493 http://dx.doi.org/10.3390/pathogens9060489 |
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