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Laboratory Diagnosis of Bovine Abortions Caused by Non-Maintenance Pathogenic Leptospira spp.: Necropsy, Serology and Molecular Study Out of a Belgian Experience

Bovine leptospirosis is a bacterial zoonotic disease caused by pathogenic Leptospira spp. The pathology and epidemiology of this infection are influenced by the numerous existing serovars and their adaptation to specific hosts. Infections by host-maintained serovars such as Hardjo are well documente...

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Autores principales: Grégoire, Fabien, Bakinahe, Raïssa, Petitjean, Thierry, Boarbi, Samira, Delooz, Laurent, Fretin, David, Saulmont, Marc, Mori, Marcella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350382/
https://www.ncbi.nlm.nih.gov/pubmed/32466444
http://dx.doi.org/10.3390/pathogens9060413
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author Grégoire, Fabien
Bakinahe, Raïssa
Petitjean, Thierry
Boarbi, Samira
Delooz, Laurent
Fretin, David
Saulmont, Marc
Mori, Marcella
author_facet Grégoire, Fabien
Bakinahe, Raïssa
Petitjean, Thierry
Boarbi, Samira
Delooz, Laurent
Fretin, David
Saulmont, Marc
Mori, Marcella
author_sort Grégoire, Fabien
collection PubMed
description Bovine leptospirosis is a bacterial zoonotic disease caused by pathogenic Leptospira spp. The pathology and epidemiology of this infection are influenced by the numerous existing serovars and their adaptation to specific hosts. Infections by host-maintained serovars such as Hardjo are well documented, unlike those from the incidental ones. In July 2014, an emerging phenomenon of an increased incidence of icteric abortions associated with leptospiral infection occurred in southern Belgium. First-line serological analyses targeting cattle-adapted serovars failed at initial diagnosis. This study provides a comprehensive description of laboratory findings—at the level of necropsy, serology and molecular diagnosis—regarding icteric and non-icteric abortions (n = 116) recorded during this time (years 2014–2015) and associated with incidental infection by serovars such as Grippotyphosa, Australis and Icterohaemorrhagiae. Based on these tests, a diagnostic pathway is proposed for these types of infection in cattle to establish an affordable but accurate diagnosis in the future. These investigations add insights into the understanding of the pathogenesis of bovine leptospirosis associated with serovars classically described as non-maintenance.
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spelling pubmed-73503822020-07-15 Laboratory Diagnosis of Bovine Abortions Caused by Non-Maintenance Pathogenic Leptospira spp.: Necropsy, Serology and Molecular Study Out of a Belgian Experience Grégoire, Fabien Bakinahe, Raïssa Petitjean, Thierry Boarbi, Samira Delooz, Laurent Fretin, David Saulmont, Marc Mori, Marcella Pathogens Article Bovine leptospirosis is a bacterial zoonotic disease caused by pathogenic Leptospira spp. The pathology and epidemiology of this infection are influenced by the numerous existing serovars and their adaptation to specific hosts. Infections by host-maintained serovars such as Hardjo are well documented, unlike those from the incidental ones. In July 2014, an emerging phenomenon of an increased incidence of icteric abortions associated with leptospiral infection occurred in southern Belgium. First-line serological analyses targeting cattle-adapted serovars failed at initial diagnosis. This study provides a comprehensive description of laboratory findings—at the level of necropsy, serology and molecular diagnosis—regarding icteric and non-icteric abortions (n = 116) recorded during this time (years 2014–2015) and associated with incidental infection by serovars such as Grippotyphosa, Australis and Icterohaemorrhagiae. Based on these tests, a diagnostic pathway is proposed for these types of infection in cattle to establish an affordable but accurate diagnosis in the future. These investigations add insights into the understanding of the pathogenesis of bovine leptospirosis associated with serovars classically described as non-maintenance. MDPI 2020-05-26 /pmc/articles/PMC7350382/ /pubmed/32466444 http://dx.doi.org/10.3390/pathogens9060413 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grégoire, Fabien
Bakinahe, Raïssa
Petitjean, Thierry
Boarbi, Samira
Delooz, Laurent
Fretin, David
Saulmont, Marc
Mori, Marcella
Laboratory Diagnosis of Bovine Abortions Caused by Non-Maintenance Pathogenic Leptospira spp.: Necropsy, Serology and Molecular Study Out of a Belgian Experience
title Laboratory Diagnosis of Bovine Abortions Caused by Non-Maintenance Pathogenic Leptospira spp.: Necropsy, Serology and Molecular Study Out of a Belgian Experience
title_full Laboratory Diagnosis of Bovine Abortions Caused by Non-Maintenance Pathogenic Leptospira spp.: Necropsy, Serology and Molecular Study Out of a Belgian Experience
title_fullStr Laboratory Diagnosis of Bovine Abortions Caused by Non-Maintenance Pathogenic Leptospira spp.: Necropsy, Serology and Molecular Study Out of a Belgian Experience
title_full_unstemmed Laboratory Diagnosis of Bovine Abortions Caused by Non-Maintenance Pathogenic Leptospira spp.: Necropsy, Serology and Molecular Study Out of a Belgian Experience
title_short Laboratory Diagnosis of Bovine Abortions Caused by Non-Maintenance Pathogenic Leptospira spp.: Necropsy, Serology and Molecular Study Out of a Belgian Experience
title_sort laboratory diagnosis of bovine abortions caused by non-maintenance pathogenic leptospira spp.: necropsy, serology and molecular study out of a belgian experience
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350382/
https://www.ncbi.nlm.nih.gov/pubmed/32466444
http://dx.doi.org/10.3390/pathogens9060413
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