A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken

Salmonella enteritidis and Salmonella pullorum belonging to Group O(9) Salmonella are major causative agents of infectious diseases in chicken. O(9) antigen as a part of lipopolysaccharide (LPS) is a predominant detected target for Salmonella infection. To identify the infection, an anti-O(9) monocl...

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Autores principales: Xia, Zemiao, Geng, Haopeng, Cai, Yuan, Wang, Yaonan, Sun, Daquan, Zhang, Jian, Pan, Zhiming, Jiao, Xin'an, Geng, Shizhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Veterinary Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350390/
https://www.ncbi.nlm.nih.gov/pubmed/32719811
http://dx.doi.org/10.3389/fvets.2020.00324
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author Xia, Zemiao
Geng, Haopeng
Cai, Yuan
Wang, Yaonan
Sun, Daquan
Zhang, Jian
Pan, Zhiming
Jiao, Xin'an
Geng, Shizhong
author_facet Xia, Zemiao
Geng, Haopeng
Cai, Yuan
Wang, Yaonan
Sun, Daquan
Zhang, Jian
Pan, Zhiming
Jiao, Xin'an
Geng, Shizhong
author_sort Xia, Zemiao
collection PubMed
description Salmonella enteritidis and Salmonella pullorum belonging to Group O(9) Salmonella are major causative agents of infectious diseases in chicken. O(9) antigen as a part of lipopolysaccharide (LPS) is a predominant detected target for Salmonella infection. To identify the infection, an anti-O(9) monoclonal antibody (McAb)-based direct competitive enzyme-linked assay (O(9) Dc-ELISA) was developed after constraints were optimized; the establishment and application of O(9) Dc-ELISA, compared to two commercial kits and plate agglutination test (PAT), showed that O(9) Dc-ELISA could screen out more positive samples than the PAT method could and produce the same agreement rates with commercial kits in terms of sensitivity in addition to strong specificity to clinical serum samples.
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spelling pubmed-73503902020-07-26 A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken Xia, Zemiao Geng, Haopeng Cai, Yuan Wang, Yaonan Sun, Daquan Zhang, Jian Pan, Zhiming Jiao, Xin'an Geng, Shizhong Front Vet Sci Veterinary Science Salmonella enteritidis and Salmonella pullorum belonging to Group O(9) Salmonella are major causative agents of infectious diseases in chicken. O(9) antigen as a part of lipopolysaccharide (LPS) is a predominant detected target for Salmonella infection. To identify the infection, an anti-O(9) monoclonal antibody (McAb)-based direct competitive enzyme-linked assay (O(9) Dc-ELISA) was developed after constraints were optimized; the establishment and application of O(9) Dc-ELISA, compared to two commercial kits and plate agglutination test (PAT), showed that O(9) Dc-ELISA could screen out more positive samples than the PAT method could and produce the same agreement rates with commercial kits in terms of sensitivity in addition to strong specificity to clinical serum samples. Frontiers Media S.A. 2020-07-03 /pmc/articles/PMC7350390/ /pubmed/32719811 http://dx.doi.org/10.3389/fvets.2020.00324 Text en Copyright © 2020 Xia, Geng, Cai, Wang, Sun, Zhang, Pan, Jiao and Geng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Xia, Zemiao
Geng, Haopeng
Cai, Yuan
Wang, Yaonan
Sun, Daquan
Zhang, Jian
Pan, Zhiming
Jiao, Xin'an
Geng, Shizhong
A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken
title A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken
title_full A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken
title_fullStr A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken
title_full_unstemmed A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken
title_short A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken
title_sort mcab-based direct competitive elisa to detect o:9 salmonella infection in chicken
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350390/
https://www.ncbi.nlm.nih.gov/pubmed/32719811
http://dx.doi.org/10.3389/fvets.2020.00324
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