Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast

OBJECTIVE: The objective of the research presented here was to determine whether autonomous replication sequences (ARS) discovered in the eccDNA replicon of glyphosate resistant Amaranthus palmeri enable self-replication in a yeast system. RESULTS: Sequence analysis of the eccDNA replicon revealed a...

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Detalles Bibliográficos
Autores principales: Molin, William T., Yaguchi, Allison, Blenner, Mark, Saski, Christopher A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350638/
https://www.ncbi.nlm.nih.gov/pubmed/32650810
http://dx.doi.org/10.1186/s13104-020-05169-0
Descripción
Sumario:OBJECTIVE: The objective of the research presented here was to determine whether autonomous replication sequences (ARS) discovered in the eccDNA replicon of glyphosate resistant Amaranthus palmeri enable self-replication in a yeast system. RESULTS: Sequence analysis of the eccDNA replicon revealed a region of sharp changes in A + T/G + C content with characteristic bending indicative of an autonomous replication sequence. Further sequence analysis revealed an extended autonomous replication sequence (EACS) in close proximity to multiple DNA unwinding element (DUE) sequences. This region of the eccDNA replicon enabled autonomous replication of an ARS-less yeast plasmid.

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