Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast

OBJECTIVE: The objective of the research presented here was to determine whether autonomous replication sequences (ARS) discovered in the eccDNA replicon of glyphosate resistant Amaranthus palmeri enable self-replication in a yeast system. RESULTS: Sequence analysis of the eccDNA replicon revealed a...

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Autores principales: Molin, William T., Yaguchi, Allison, Blenner, Mark, Saski, Christopher A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350638/
https://www.ncbi.nlm.nih.gov/pubmed/32650810
http://dx.doi.org/10.1186/s13104-020-05169-0
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author Molin, William T.
Yaguchi, Allison
Blenner, Mark
Saski, Christopher A.
author_facet Molin, William T.
Yaguchi, Allison
Blenner, Mark
Saski, Christopher A.
author_sort Molin, William T.
collection PubMed
description OBJECTIVE: The objective of the research presented here was to determine whether autonomous replication sequences (ARS) discovered in the eccDNA replicon of glyphosate resistant Amaranthus palmeri enable self-replication in a yeast system. RESULTS: Sequence analysis of the eccDNA replicon revealed a region of sharp changes in A + T/G + C content with characteristic bending indicative of an autonomous replication sequence. Further sequence analysis revealed an extended autonomous replication sequence (EACS) in close proximity to multiple DNA unwinding element (DUE) sequences. This region of the eccDNA replicon enabled autonomous replication of an ARS-less yeast plasmid.
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spelling pubmed-73506382020-07-14 Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast Molin, William T. Yaguchi, Allison Blenner, Mark Saski, Christopher A. BMC Res Notes Research Note OBJECTIVE: The objective of the research presented here was to determine whether autonomous replication sequences (ARS) discovered in the eccDNA replicon of glyphosate resistant Amaranthus palmeri enable self-replication in a yeast system. RESULTS: Sequence analysis of the eccDNA replicon revealed a region of sharp changes in A + T/G + C content with characteristic bending indicative of an autonomous replication sequence. Further sequence analysis revealed an extended autonomous replication sequence (EACS) in close proximity to multiple DNA unwinding element (DUE) sequences. This region of the eccDNA replicon enabled autonomous replication of an ARS-less yeast plasmid. BioMed Central 2020-07-10 /pmc/articles/PMC7350638/ /pubmed/32650810 http://dx.doi.org/10.1186/s13104-020-05169-0 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Molin, William T.
Yaguchi, Allison
Blenner, Mark
Saski, Christopher A.
Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast
title Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast
title_full Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast
title_fullStr Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast
title_full_unstemmed Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast
title_short Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast
title_sort autonomous replication sequences from the amaranthus palmeri eccdna replicon enable replication in yeast
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350638/
https://www.ncbi.nlm.nih.gov/pubmed/32650810
http://dx.doi.org/10.1186/s13104-020-05169-0
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