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Incorporation of the first and second heart fields and prospective fate of the straight heart tube via in vivo labeling of chicken embryos
Recent discoveries of at least two heart fields and dynamic nature of cardiac development as well as controversies regarding the participation of heart fields in development of different heart structures led us to investigate the dynamics of incorporation of the first and second heart fields and pro...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351196/ https://www.ncbi.nlm.nih.gov/pubmed/32649674 http://dx.doi.org/10.1371/journal.pone.0234069 |
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author | Laura, Villavicencio Guzmán Marcela, Salazar García Ricardo, Jaime Cruz Roberto, Lazzarini Filiberto, Toledano-Toledano Sánchez Gómez, Concepción |
author_facet | Laura, Villavicencio Guzmán Marcela, Salazar García Ricardo, Jaime Cruz Roberto, Lazzarini Filiberto, Toledano-Toledano Sánchez Gómez, Concepción |
author_sort | Laura, Villavicencio Guzmán |
collection | PubMed |
description | Recent discoveries of at least two heart fields and dynamic nature of cardiac development as well as controversies regarding the participation of heart fields in development of different heart structures led us to investigate the dynamics of incorporation of the first and second heart fields and prospective fate of the straight heart tube by labeling chicken embryos in vivo with the fluorescent lipophilic dye DiI. The cephalic and caudal limits of the anterior and posterior segments of the straight heart tube were labeled in two groups of embryos. Labels were tracked along the “C,” “S,” and “U” loops up to the tetracavitary or mature heart (n = 30 embryos/group; torsion and looping stage). To determine whether the atria and atrioventricular canal are derived from the first heart field the straight heart tube was cultured in vitro and immunodetection of Sox-9 and troponin I was performed to identify the mesenchymal and myocardial lineages respectively. Proliferating cell nuclear antigen (PCNA) immunodetection was used to determine the involvement of cell proliferation in heart tube development during torsion and looping. Embryological constitution of the straight heart tube and heart looping (C, S, and U) were not consistent with current descriptions. In fact, right ventricle precursors were absent in the straight heart tube derived from the first heart field. During torsion and looping, the cephalic segment of the straight heart tube gradually shifted into the heart tube until it was located at the myocardial interventricular septum in the tetracavitary heart. In contrast, the caudal segment of the straight heart tube was elongated and remodeled to become the first heart field derived left ventricle and the proximal part of the ventricular inlets. The ventricular outflows, right ventricle, distal part of the ventricular inlets, and atria developed from the second heart field. |
format | Online Article Text |
id | pubmed-7351196 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-73511962020-07-22 Incorporation of the first and second heart fields and prospective fate of the straight heart tube via in vivo labeling of chicken embryos Laura, Villavicencio Guzmán Marcela, Salazar García Ricardo, Jaime Cruz Roberto, Lazzarini Filiberto, Toledano-Toledano Sánchez Gómez, Concepción PLoS One Research Article Recent discoveries of at least two heart fields and dynamic nature of cardiac development as well as controversies regarding the participation of heart fields in development of different heart structures led us to investigate the dynamics of incorporation of the first and second heart fields and prospective fate of the straight heart tube by labeling chicken embryos in vivo with the fluorescent lipophilic dye DiI. The cephalic and caudal limits of the anterior and posterior segments of the straight heart tube were labeled in two groups of embryos. Labels were tracked along the “C,” “S,” and “U” loops up to the tetracavitary or mature heart (n = 30 embryos/group; torsion and looping stage). To determine whether the atria and atrioventricular canal are derived from the first heart field the straight heart tube was cultured in vitro and immunodetection of Sox-9 and troponin I was performed to identify the mesenchymal and myocardial lineages respectively. Proliferating cell nuclear antigen (PCNA) immunodetection was used to determine the involvement of cell proliferation in heart tube development during torsion and looping. Embryological constitution of the straight heart tube and heart looping (C, S, and U) were not consistent with current descriptions. In fact, right ventricle precursors were absent in the straight heart tube derived from the first heart field. During torsion and looping, the cephalic segment of the straight heart tube gradually shifted into the heart tube until it was located at the myocardial interventricular septum in the tetracavitary heart. In contrast, the caudal segment of the straight heart tube was elongated and remodeled to become the first heart field derived left ventricle and the proximal part of the ventricular inlets. The ventricular outflows, right ventricle, distal part of the ventricular inlets, and atria developed from the second heart field. Public Library of Science 2020-07-10 /pmc/articles/PMC7351196/ /pubmed/32649674 http://dx.doi.org/10.1371/journal.pone.0234069 Text en © 2020 Laura et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Laura, Villavicencio Guzmán Marcela, Salazar García Ricardo, Jaime Cruz Roberto, Lazzarini Filiberto, Toledano-Toledano Sánchez Gómez, Concepción Incorporation of the first and second heart fields and prospective fate of the straight heart tube via in vivo labeling of chicken embryos |
title | Incorporation of the first and second heart fields and prospective fate of the straight heart tube via in vivo labeling of chicken embryos |
title_full | Incorporation of the first and second heart fields and prospective fate of the straight heart tube via in vivo labeling of chicken embryos |
title_fullStr | Incorporation of the first and second heart fields and prospective fate of the straight heart tube via in vivo labeling of chicken embryos |
title_full_unstemmed | Incorporation of the first and second heart fields and prospective fate of the straight heart tube via in vivo labeling of chicken embryos |
title_short | Incorporation of the first and second heart fields and prospective fate of the straight heart tube via in vivo labeling of chicken embryos |
title_sort | incorporation of the first and second heart fields and prospective fate of the straight heart tube via in vivo labeling of chicken embryos |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351196/ https://www.ncbi.nlm.nih.gov/pubmed/32649674 http://dx.doi.org/10.1371/journal.pone.0234069 |
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