Cargando…
The effects of synthetic orally administrated insulin nanoparticles in comparison to injectable insulin on the renal function markers of type 1- diabetic rats
OBJECTIVE(S): Injectable insulin is the most widely used therapy in patients with type 1 diabetes which has several disadvantages. The present study was aimed to evaluate the efficacy of injectable insulin on diabetes mellitus-related complications in comparison to orally encapsulated insulin nanopa...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351444/ https://www.ncbi.nlm.nih.gov/pubmed/32695298 http://dx.doi.org/10.22038/ijbms.2020.42292.9985 |
_version_ | 1783557445450203136 |
---|---|
author | Kheiripour, Nejat Alipoor, Behnam Ranjbar, Akram Pourfarjam, Yasin Kazemi Najafabadi, Farzaneh Dehkhodaei, Narges Farhadiannezhad, Masoumeh Ghasemi, Hassan |
author_facet | Kheiripour, Nejat Alipoor, Behnam Ranjbar, Akram Pourfarjam, Yasin Kazemi Najafabadi, Farzaneh Dehkhodaei, Narges Farhadiannezhad, Masoumeh Ghasemi, Hassan |
author_sort | Kheiripour, Nejat |
collection | PubMed |
description | OBJECTIVE(S): Injectable insulin is the most widely used therapy in patients with type 1 diabetes which has several disadvantages. The present study was aimed to evaluate the efficacy of injectable insulin on diabetes mellitus-related complications in comparison to orally encapsulated insulin nanoparticles. MATERIALS AND METHODS: This study involved 42 Wistar rats separated into 5 groups, including control (C), diabetic control (D), diabetic receiving regular insulin (INS), diabetic receiving encapsulated insulin nanoparticle (INP), and diabetic receiving chitosan for two months. Biochemical parameters in serum and urine were measured using spectrophotometric or ELISA methods. mRNA levels of kidney injury molecule 1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) were evaluated using quantitative PCR. RESULTS: There were no significant differences between the two forms of insulin in controlling the glycemic condition (P-value>0.05), but oral INP was more effective in correcting diabetic dyslipidemia in comparison to injectable insulin (P-value<0.05). Urine volume and creatinine excretion were significantly modulated by insulin and oral INP in diabetic groups (P-value<0.05), although the effects of INP on the modulation of execration of urea, acid uric, and albumin was more dramatic. Oral INP caused a significant decrease in urine concentration of KIM-1 and NGAL as well as expression of KIM-1 in renal tissue (P-value<0.05). CONCLUSION: Our results suggested that oral INP is more effective than injectable insulin in modulation of urine and serum diabetic-related parameters. |
format | Online Article Text |
id | pubmed-7351444 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-73514442020-07-20 The effects of synthetic orally administrated insulin nanoparticles in comparison to injectable insulin on the renal function markers of type 1- diabetic rats Kheiripour, Nejat Alipoor, Behnam Ranjbar, Akram Pourfarjam, Yasin Kazemi Najafabadi, Farzaneh Dehkhodaei, Narges Farhadiannezhad, Masoumeh Ghasemi, Hassan Iran J Basic Med Sci Original Article OBJECTIVE(S): Injectable insulin is the most widely used therapy in patients with type 1 diabetes which has several disadvantages. The present study was aimed to evaluate the efficacy of injectable insulin on diabetes mellitus-related complications in comparison to orally encapsulated insulin nanoparticles. MATERIALS AND METHODS: This study involved 42 Wistar rats separated into 5 groups, including control (C), diabetic control (D), diabetic receiving regular insulin (INS), diabetic receiving encapsulated insulin nanoparticle (INP), and diabetic receiving chitosan for two months. Biochemical parameters in serum and urine were measured using spectrophotometric or ELISA methods. mRNA levels of kidney injury molecule 1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) were evaluated using quantitative PCR. RESULTS: There were no significant differences between the two forms of insulin in controlling the glycemic condition (P-value>0.05), but oral INP was more effective in correcting diabetic dyslipidemia in comparison to injectable insulin (P-value<0.05). Urine volume and creatinine excretion were significantly modulated by insulin and oral INP in diabetic groups (P-value<0.05), although the effects of INP on the modulation of execration of urea, acid uric, and albumin was more dramatic. Oral INP caused a significant decrease in urine concentration of KIM-1 and NGAL as well as expression of KIM-1 in renal tissue (P-value<0.05). CONCLUSION: Our results suggested that oral INP is more effective than injectable insulin in modulation of urine and serum diabetic-related parameters. Mashhad University of Medical Sciences 2020-06 /pmc/articles/PMC7351444/ /pubmed/32695298 http://dx.doi.org/10.22038/ijbms.2020.42292.9985 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kheiripour, Nejat Alipoor, Behnam Ranjbar, Akram Pourfarjam, Yasin Kazemi Najafabadi, Farzaneh Dehkhodaei, Narges Farhadiannezhad, Masoumeh Ghasemi, Hassan The effects of synthetic orally administrated insulin nanoparticles in comparison to injectable insulin on the renal function markers of type 1- diabetic rats |
title | The effects of synthetic orally administrated insulin nanoparticles in comparison to injectable insulin on the renal function markers of type 1- diabetic rats |
title_full | The effects of synthetic orally administrated insulin nanoparticles in comparison to injectable insulin on the renal function markers of type 1- diabetic rats |
title_fullStr | The effects of synthetic orally administrated insulin nanoparticles in comparison to injectable insulin on the renal function markers of type 1- diabetic rats |
title_full_unstemmed | The effects of synthetic orally administrated insulin nanoparticles in comparison to injectable insulin on the renal function markers of type 1- diabetic rats |
title_short | The effects of synthetic orally administrated insulin nanoparticles in comparison to injectable insulin on the renal function markers of type 1- diabetic rats |
title_sort | effects of synthetic orally administrated insulin nanoparticles in comparison to injectable insulin on the renal function markers of type 1- diabetic rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351444/ https://www.ncbi.nlm.nih.gov/pubmed/32695298 http://dx.doi.org/10.22038/ijbms.2020.42292.9985 |
work_keys_str_mv | AT kheiripournejat theeffectsofsyntheticorallyadministratedinsulinnanoparticlesincomparisontoinjectableinsulinontherenalfunctionmarkersoftype1diabeticrats AT alipoorbehnam theeffectsofsyntheticorallyadministratedinsulinnanoparticlesincomparisontoinjectableinsulinontherenalfunctionmarkersoftype1diabeticrats AT ranjbarakram theeffectsofsyntheticorallyadministratedinsulinnanoparticlesincomparisontoinjectableinsulinontherenalfunctionmarkersoftype1diabeticrats AT pourfarjamyasin theeffectsofsyntheticorallyadministratedinsulinnanoparticlesincomparisontoinjectableinsulinontherenalfunctionmarkersoftype1diabeticrats AT kazeminajafabadifarzaneh theeffectsofsyntheticorallyadministratedinsulinnanoparticlesincomparisontoinjectableinsulinontherenalfunctionmarkersoftype1diabeticrats AT dehkhodaeinarges theeffectsofsyntheticorallyadministratedinsulinnanoparticlesincomparisontoinjectableinsulinontherenalfunctionmarkersoftype1diabeticrats AT farhadiannezhadmasoumeh theeffectsofsyntheticorallyadministratedinsulinnanoparticlesincomparisontoinjectableinsulinontherenalfunctionmarkersoftype1diabeticrats AT ghasemihassan theeffectsofsyntheticorallyadministratedinsulinnanoparticlesincomparisontoinjectableinsulinontherenalfunctionmarkersoftype1diabeticrats AT kheiripournejat effectsofsyntheticorallyadministratedinsulinnanoparticlesincomparisontoinjectableinsulinontherenalfunctionmarkersoftype1diabeticrats AT alipoorbehnam effectsofsyntheticorallyadministratedinsulinnanoparticlesincomparisontoinjectableinsulinontherenalfunctionmarkersoftype1diabeticrats AT ranjbarakram effectsofsyntheticorallyadministratedinsulinnanoparticlesincomparisontoinjectableinsulinontherenalfunctionmarkersoftype1diabeticrats AT pourfarjamyasin effectsofsyntheticorallyadministratedinsulinnanoparticlesincomparisontoinjectableinsulinontherenalfunctionmarkersoftype1diabeticrats AT kazeminajafabadifarzaneh effectsofsyntheticorallyadministratedinsulinnanoparticlesincomparisontoinjectableinsulinontherenalfunctionmarkersoftype1diabeticrats AT dehkhodaeinarges effectsofsyntheticorallyadministratedinsulinnanoparticlesincomparisontoinjectableinsulinontherenalfunctionmarkersoftype1diabeticrats AT farhadiannezhadmasoumeh effectsofsyntheticorallyadministratedinsulinnanoparticlesincomparisontoinjectableinsulinontherenalfunctionmarkersoftype1diabeticrats AT ghasemihassan effectsofsyntheticorallyadministratedinsulinnanoparticlesincomparisontoinjectableinsulinontherenalfunctionmarkersoftype1diabeticrats |