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CMTM5表达对多发性骨髓瘤细胞增殖的影响及其机制研究
OBJECTIVE: To investigate the mechanism of chemokine-like factor superfamily member (CMTM) 5 on the proliferation of multiple myeloma cells. METHODS: RT-qPCR method was used to detect the expression and correlation of CMTM5, caspase3 and caspase9 in U266 after decitabine demethylation treatment; U26...
Formato: | Online Artículo Texto |
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Lenguaje: | English |
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Editorial office of Chinese Journal of Hematology
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351701/ https://www.ncbi.nlm.nih.gov/pubmed/30704230 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2019.01.011 |
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collection | PubMed |
description | OBJECTIVE: To investigate the mechanism of chemokine-like factor superfamily member (CMTM) 5 on the proliferation of multiple myeloma cells. METHODS: RT-qPCR method was used to detect the expression and correlation of CMTM5, caspase3 and caspase9 in U266 after decitabine demethylation treatment; U266 transfected with pcDNA3.1 plasmid overexpressed CMTM5, then cell proliferation activity was detected by CCK-8 assay. RESULTS: Compared with the control group, the low-dose demethylation treatment increased mRNA expression of CMTM5, caspase3, and caspase9 in U266, and showed a time-dependent (P<0.01). The up-trend of CMTM5, caspase3, and caspase9 in the high-demethylation drug treatment group was more significant and also showed time-dependent (P<0.001); There was a significant positive correlation between CMTM5 and caspase3 (r=0.937) and caspase9 (r=0.945) in each group (P<0.001). After transfection of U266 with the pcDNA3.1-CMTM5 plasmid, overexpression of CMTM5 inhibited the cell proliferation activity compared with the control and pcDNA3.1-vector group. CONCLUSION: Decitabine has a reductive effect on the low level of CMTM5 in U266 cells, and its recovery level is significantly positively correlated with caspase 3 and caspase9. Re-expression of CMTM5 inhibits the proliferative activity of U266. |
format | Online Article Text |
id | pubmed-7351701 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Editorial office of Chinese Journal of Hematology |
record_format | MEDLINE/PubMed |
spelling | pubmed-73517012020-07-16 CMTM5表达对多发性骨髓瘤细胞增殖的影响及其机制研究 Zhonghua Xue Ye Xue Za Zhi 论著 OBJECTIVE: To investigate the mechanism of chemokine-like factor superfamily member (CMTM) 5 on the proliferation of multiple myeloma cells. METHODS: RT-qPCR method was used to detect the expression and correlation of CMTM5, caspase3 and caspase9 in U266 after decitabine demethylation treatment; U266 transfected with pcDNA3.1 plasmid overexpressed CMTM5, then cell proliferation activity was detected by CCK-8 assay. RESULTS: Compared with the control group, the low-dose demethylation treatment increased mRNA expression of CMTM5, caspase3, and caspase9 in U266, and showed a time-dependent (P<0.01). The up-trend of CMTM5, caspase3, and caspase9 in the high-demethylation drug treatment group was more significant and also showed time-dependent (P<0.001); There was a significant positive correlation between CMTM5 and caspase3 (r=0.937) and caspase9 (r=0.945) in each group (P<0.001). After transfection of U266 with the pcDNA3.1-CMTM5 plasmid, overexpression of CMTM5 inhibited the cell proliferation activity compared with the control and pcDNA3.1-vector group. CONCLUSION: Decitabine has a reductive effect on the low level of CMTM5 in U266 cells, and its recovery level is significantly positively correlated with caspase 3 and caspase9. Re-expression of CMTM5 inhibits the proliferative activity of U266. Editorial office of Chinese Journal of Hematology 2019-01 /pmc/articles/PMC7351701/ /pubmed/30704230 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2019.01.011 Text en 2019年版权归中华医学会所有 http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution 3.0 License (CC-BY-NC). The Copyright own by Publisher. Without authorization, shall not reprint, except this publication article, shall not use this publication format design. Unless otherwise stated, all articles published in this journal do not represent the views of the Chinese Medical Association or the editorial board of this journal. |
spellingShingle | 论著 CMTM5表达对多发性骨髓瘤细胞增殖的影响及其机制研究 |
title | CMTM5表达对多发性骨髓瘤细胞增殖的影响及其机制研究 |
title_full | CMTM5表达对多发性骨髓瘤细胞增殖的影响及其机制研究 |
title_fullStr | CMTM5表达对多发性骨髓瘤细胞增殖的影响及其机制研究 |
title_full_unstemmed | CMTM5表达对多发性骨髓瘤细胞增殖的影响及其机制研究 |
title_short | CMTM5表达对多发性骨髓瘤细胞增殖的影响及其机制研究 |
title_sort | cmtm5表达对多发性骨髓瘤细胞增殖的影响及其机制研究 |
topic | 论著 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351701/ https://www.ncbi.nlm.nih.gov/pubmed/30704230 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2019.01.011 |
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