Cargando…

Modular microfluidics enables kinetic insight from time-resolved cryo-EM

Mechanistic understanding of biochemical reactions requires structural and kinetic characterization of the underlying chemical processes. However, no single experimental technique can provide this information in a broadly applicable manner and thus structural studies of static macromolecules are oft...

Descripción completa

Detalles Bibliográficos
Autores principales:	Mäeots, Märt-Erik, Lee, Byungjin, Nans, Andrea, Jeong, Seung-Geun, Esfahani, Mohammad M. N., Ding, Shan, Smith, Daniel J., Lee, Chang-Soo, Lee, Sung Sik, Peter, Matthias, Enchev, Radoslav I.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351747/ https://www.ncbi.nlm.nih.gov/pubmed/32651368 http://dx.doi.org/10.1038/s41467-020-17230-4

Ejemplares similares

Structural dynamics: review of time-resolved cryo-EM
por: Mäeots, Märt-Erik, et al.
Publicado: (2022)

Time-resolved cryoEM using Spotiton
por: Dandey, Venkata P., et al.
Publicado: (2020)

On-grid and in-flow mixing for time-resolved cryo-EM
por: Klebl, David P., et al.
Publicado: (2021)

Measurement of Atom Resolvability in CryoEM Maps with Q-scores
por: Pintilie, Grigore, et al.
Publicado: (2020)

Topological crossing in the misfolded Tetrahymena ribozyme resolved by cryo-EM
por: Li, Shanshan, et al.
Publicado: (2022)

Cryo-EM structure of a licensed DNA replication origin
por: Abid Ali, Ferdos, et al.
Publicado: (2017)

Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses
por: Wang, Ling, et al.
Publicado: (2020)

Microfluidic protein isolation and sample preparation for high-resolution cryo-EM
por: Schmidli, Claudio, et al.
Publicado: (2019)

Fast viral dynamics revealed by microsecond time-resolved cryo-EM
por: Harder, Oliver F., et al.
Publicado: (2023)

Cryo-EM tomography and automatic segmentation delineate modular structures in the postsynaptic density
por: Jung, Jae Hoon, et al.
Publicado: (2023)

Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM
por: Antanasijevic, Aleksandar, et al.
Publicado: (2021)

A cryo-EM grid preparation device for time-resolved structural studies
por: Kontziampasis, Dimitrios, et al.
Publicado: (2019)

Time-resolved cryo-EM visualizes ribosomal translocation with EF-G and GTP
por: Carbone, Christine E., et al.
Publicado: (2021)

USP14-regulated allostery of the human proteasome by time-resolved cryo-EM
por: Zhang, Shuwen, et al.
Publicado: (2022)

Late steps in bacterial translation initiation visualized using time-resolved cryo-EM
por: Kaledhonkar, Sandip, et al.
Publicado: (2019)

Cryo-EM reveals an entangled kinetic trap in the folding of a catalytic RNA
por: Bonilla, Steve L., et al.
Publicado: (2022)

Improving Resolution and Resolvability of Single Particle CryoEM Structures using Gaussian Mixture Models
por: Chen, Muyuan, et al.
Publicado: (2023)

Cryo-EM structure of the deltaretroviral intasome in complex with the PP2A regulatory subunit B56γ
por: Barski, Michał S., et al.
Publicado: (2020)

Cryo-EM structure of the mitochondrial calcium uniporter
por: Nguyen, Nam X., et al.
Publicado: (2018)

A snapshot of cryo‐EM
por: Skiniotis, Georgios
Publicado: (2016)

Interpreting the cryo-EM map
por: Rosenthal, Peter B.
Publicado: (2019)

COVID-19 and cryo-EM
por: Subramaniam, Sriram
Publicado: (2020)

Cryo-EM at ACA 2022
por: Subramaniam, Sriram, et al.
Publicado: (2022)

Tools for the cryo-EM gold rush: going from the cryo-EM map to the atomistic model
por: Kim, Doo Nam, et al.
Publicado: (2017)

Connexin-46/50 in a dynamic lipid environment resolved by CryoEM at 1.9 Å
por: Flores, Jonathan A., et al.
Publicado: (2020)

Cryo‐EM Resolves Molecular Recognition Of An Optojasp Photoswitch Bound To Actin Filaments In Both Switch States
por: Pospich, Sabrina, et al.
Publicado: (2021)

Environmentally Ultrasensitive Fluorine Probe to Resolve Protein Conformational Ensembles by (19)F NMR and Cryo-EM
por: Huang, Yun, et al.
Publicado: (2023)

Microfluidic preparation of monodisperse polymeric microspheres coated with silica nanoparticles
por: Kim, Dong-Yeong, et al.
Publicado: (2018)

Time-resolved cryo-EM (TR-EM) analysis of substrate polyubiquitination by the RING E3 anaphase-promoting complex/cyclosome (APC/C)
por: Bodrug, Tatyana, et al.
Publicado: (2023)

Modular and Self-Contained Microfluidic Analytical Platforms Enabled by Magnetorheological Elastomer Microactuators
por: Zhang, Yuxin, et al.
Publicado: (2021)

RosettaES: a sampling strategy enabling automated interpretation of difficult cryo-EM maps
por: Frenz, Brandon, et al.
Publicado: (2017)

Ribosome preparation from turquoise killifish skeletal muscle for cryo-EM
por: Lee, Seongsin, et al.
Publicado: (2022)

Exploring applications of crowdsourcing to cryo-EM
por: Bruggemann, Jacob, et al.
Publicado: (2018)

Cryo-EM Structure of the Exocyst Complex
por: Mei, Kunrong, et al.
Publicado: (2018)

Density modification of cryo-EM maps
por: Terwilliger, Thomas C., et al.
Publicado: (2020)

Fast multiscale reconstruction for Cryo-EM()
por: Donati, Laurène, et al.
Publicado: (2018)

Cryo‐EM targets in CASP14
por: Cragnolini, Tristan, et al.
Publicado: (2021)

Cryo-EM structure of the SEA complex
por: Tafur, Lucas, et al.
Publicado: (2022)

Cryo‐EM structures of coagulation factors
por: Di Cera, Enrico, et al.
Publicado: (2022)

AlphaFold2 and CryoEM: Revisiting CryoEM modeling in near-atomic resolution density maps
por: Hryc, Corey F., et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_hg60enm7hcfacemqu7uo4vuob8