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Molecular and evolutionary analysis of dengue virus serotype 2 isolates from Korean travelers in 2015

In Korea, dengue infection has been frequently reported in travelers to tropical and subtropical countries. Global warming increases the probability of autochthonous dengue outbreaks in Korea. In this report, the molecular and evolutionary properties of four dengue virus (DENV) type 2 isolates from...

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Autores principales: Hwang, Eun-Ha, Kim, Green, Oh, Hanseul, An, You Jung, Kim, Jiyeon, Kim, Jung Heon, Hwang, Eung-Soo, Park, Jong-Hwan, Hong, JungJoo, Koo, Bon-Sang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351809/
https://www.ncbi.nlm.nih.gov/pubmed/32409874
http://dx.doi.org/10.1007/s00705-020-04653-z
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author Hwang, Eun-Ha
Kim, Green
Oh, Hanseul
An, You Jung
Kim, Jiyeon
Kim, Jung Heon
Hwang, Eung-Soo
Park, Jong-Hwan
Hong, JungJoo
Koo, Bon-Sang
author_facet Hwang, Eun-Ha
Kim, Green
Oh, Hanseul
An, You Jung
Kim, Jiyeon
Kim, Jung Heon
Hwang, Eung-Soo
Park, Jong-Hwan
Hong, JungJoo
Koo, Bon-Sang
author_sort Hwang, Eun-Ha
collection PubMed
description In Korea, dengue infection has been frequently reported in travelers to tropical and subtropical countries. Global warming increases the probability of autochthonous dengue outbreaks in Korea. In this report, the molecular and evolutionary properties of four dengue virus (DENV) type 2 isolates from Korean overseas travelers were examined. Three of these isolates were classified as Cosmopolitan genotypes and further divided into sublineages 1 (43,253, 43,254) and 2 (43,248), while the other isolate (KBPV-VR29) was related to American genotypes. The variable amino acid motifs related to virulence and replication were identified in the structural and non-structural proteins. A negative selection mechanism was clearly verified in all of the DENV proteins. Potential recombination events were identified in the NS5 protein of the XSBN10 strain. The substitution rate (5.32 × 10(−4) substitutions per site) and the time of the most recent common ancestor (TMRCA) for each evolutionary group were determined by the Bayesian skyline coalescent method. This study shows that DENV type 2 strains with distinct phylogenetic, evolutionary, and virulence characteristics have been introduced into Korea by overseas travelers and have the potential to trigger autochthonous dengue outbreaks. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00705-020-04653-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-73518092020-07-16 Molecular and evolutionary analysis of dengue virus serotype 2 isolates from Korean travelers in 2015 Hwang, Eun-Ha Kim, Green Oh, Hanseul An, You Jung Kim, Jiyeon Kim, Jung Heon Hwang, Eung-Soo Park, Jong-Hwan Hong, JungJoo Koo, Bon-Sang Arch Virol Original Article In Korea, dengue infection has been frequently reported in travelers to tropical and subtropical countries. Global warming increases the probability of autochthonous dengue outbreaks in Korea. In this report, the molecular and evolutionary properties of four dengue virus (DENV) type 2 isolates from Korean overseas travelers were examined. Three of these isolates were classified as Cosmopolitan genotypes and further divided into sublineages 1 (43,253, 43,254) and 2 (43,248), while the other isolate (KBPV-VR29) was related to American genotypes. The variable amino acid motifs related to virulence and replication were identified in the structural and non-structural proteins. A negative selection mechanism was clearly verified in all of the DENV proteins. Potential recombination events were identified in the NS5 protein of the XSBN10 strain. The substitution rate (5.32 × 10(−4) substitutions per site) and the time of the most recent common ancestor (TMRCA) for each evolutionary group were determined by the Bayesian skyline coalescent method. This study shows that DENV type 2 strains with distinct phylogenetic, evolutionary, and virulence characteristics have been introduced into Korea by overseas travelers and have the potential to trigger autochthonous dengue outbreaks. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00705-020-04653-z) contains supplementary material, which is available to authorized users. Springer Vienna 2020-05-14 2020 /pmc/articles/PMC7351809/ /pubmed/32409874 http://dx.doi.org/10.1007/s00705-020-04653-z Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Hwang, Eun-Ha
Kim, Green
Oh, Hanseul
An, You Jung
Kim, Jiyeon
Kim, Jung Heon
Hwang, Eung-Soo
Park, Jong-Hwan
Hong, JungJoo
Koo, Bon-Sang
Molecular and evolutionary analysis of dengue virus serotype 2 isolates from Korean travelers in 2015
title Molecular and evolutionary analysis of dengue virus serotype 2 isolates from Korean travelers in 2015
title_full Molecular and evolutionary analysis of dengue virus serotype 2 isolates from Korean travelers in 2015
title_fullStr Molecular and evolutionary analysis of dengue virus serotype 2 isolates from Korean travelers in 2015
title_full_unstemmed Molecular and evolutionary analysis of dengue virus serotype 2 isolates from Korean travelers in 2015
title_short Molecular and evolutionary analysis of dengue virus serotype 2 isolates from Korean travelers in 2015
title_sort molecular and evolutionary analysis of dengue virus serotype 2 isolates from korean travelers in 2015
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351809/
https://www.ncbi.nlm.nih.gov/pubmed/32409874
http://dx.doi.org/10.1007/s00705-020-04653-z
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